FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002150388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187813
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG25891.3784988259598643Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG21591.1495476883921774Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19551.0409290091740189No Hit
GTACAGAACCGCGCCAGTCGCGTGCTAGACGAAACCGACGCGGCCTTACGGCTAGGAGGATCCGCGGAACGCCGG12790.680996523137376No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT11940.63573874012981Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGCCGGGCCTGGTCGAAGCTCGTTCGCGCGAGCGGACTCTGGGCCCGCGCTCCGGCGTTCCGCGGATCCTCCTA11820.6293494060581536No Hit
GTTCTGAGCTGACCGTTGAACGGCGGTCGTACAGAACCGCGCCAGTCGCGTGCTAGACGAAACCGACGCGGCCTT11190.5958054021819575No Hit
CCCCTTGTCCGTGCCAGTTCTGAGCTGACCGTTGAACGGCGGTCGTACAGAACCGCGCCAGTCGCGTGCTAGACG10990.5851565120625303No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10250.5457556186206493No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC9920.5281849499235942Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8470.45098049655774625No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG7360.3918791563949247Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCCGCGAGGGCCATCGCAATGCTTTGTTTTA7340.390814267382982No Hit
CTCTGGGCCCGCGCTCCGGCGTTCCGCGGATCCTCCTAGCCGTAAGGCCGCGTCGGTTTCGTCTAGCACGCGACT7310.3892169338650679No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG7180.3822951552874402Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC6900.3673867091202419Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT6100.3247911486425327Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCCGCGAGGGCCATCGCAATGCTTTGTTTTAATTAGA6070.32319381512461864No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA5790.3082853689574204Clontech Universal Primer Mix Long (96% over 26bp)
GCCTTACGGCTAGGAGGATCCGCGGAACGCCGGAGCGCGGGCCCAGAGTCCGCTCGCGCGAACGAGCTTCGACCA5460.29071470026036533No Hit
TGCCAGTTCTGAGCTGACCGTTGAACGGCGGTCGTACAGAACCGCGCCAGTCGCGTGCTAGACGAAACCGACGCG5140.2736764760692817No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG5010.2667546974916539Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGCCCAGAGTCCGCTCGCGCGAACGAGCTTCGACCAGGCCCGGCACCGGCCGCATCCGCTTCCCCTCCAAACCCG4940.26302758594985437No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC4870.2593004744080548Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4790.2550409183602839No Hit
GCTCTGAGGACCGGGGCGCGTCGGGTTTGGAGGGGAAGCGGATGCGGCCGGTGCCGGGCCTGGTCGAAGCTCGTT4560.2427946947229425No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCCGCGAGGGCCATCGCAATGCTTTGTTTTAATTAGACAGTCGG4440.23640536065128612No Hit
GTCGTACAGAACCGCGCCAGTCGCGTGCTAGACGAAACCGACGCGGCCTTACGGCTAGGAGGATCCGCGGAACGC4380.23321069361545793No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG4300.228951137567687Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCTGTACGACCGCCGTTCAACGGTCAGCTCAGAACTGGCACGGACAAGGGGAATCCGACTGTCTAATTAAAAC4140.22043202547214513No Hit
AAATCACATTGCGTCAACACCCGCGAGGGCCATCGCAATGCTTTGTTTTAATTAGACAGTCGGATTCCCCTTGTC4070.21670491393034563No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC3860.20552357930494694Clontech Universal Primer Mix Long (96% over 26bp)
GGCCATCGCAATGCTTTGTTTTAATTAGACAGTCGGATTCCCCTTGTCCGTGCCAGTTCTGAGCTGACCGTTGAA3780.20126402325717602No Hit
GGCTAGGAGGATCCGCGGAACGCCGGAGCGCGGGCCCAGAGTCCGCTCGCGCGAACGAGCTTCGACCAGGCCCGG3700.19700446720940512No Hit
GAATAATGTAGGTAAGGGAAGTCGGCAAATTGGATCCGTAACTTCGGAATAAGGATTGGCTCTGAGGACCGGGGC3680.19593957819746236No Hit
TCGCAATGCTTTGTTTTAATTAGACAGTCGGATTCCCCTTGTCCGTGCCAGTTCTGAGCTGACCGTTGAACGGCG3590.1911475776437201No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC3550.18901779961983464Clontech Universal Primer Mix Long (96% over 26bp)
CTCCTAGCCGTAAGGCCGCGTCGGTTTCGTCTAGCACGCGACTGGCGCGGTTCTGTACGACCGCCGTTCAACGGT3520.18742046610192054No Hit
GGCCCGGCACCGGCCGCATCCGCTTCCCCTCCAAACCCGACGCGCCCCGGTCCTCAGAGCCAATCCTTATTCCGA3500.1863555770899778No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCCGCGAGGGCCATCGCAATGCTTTGTTTTAA3490.18582313258400643No Hit
TAATTAGACAGTCGGATTCCCCTTGTCCGTGCCAGTTCTGAGCTGACCGTTGAACGGCGGTCGTACAGAACCGCG3490.18582313258400643No Hit
GCGTCAACACCCGCGAGGGCCATCGCAATGCTTTGTTTTAATTAGACAGTCGGATTCCCCTTGTCCGTGCCAGTT3430.18262846554817824No Hit
CGGCTAGGAGGATCCGCGGAACGCCGGAGCGCGGGCCCAGAGTCCGCTCGCGCGAACGAGCTTCGACCAGGCCCG3120.16612268586306592No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCCGCGAGGGCCATCGCAATGCTTTGTTTT2940.15653868475558136No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2750.14642223914212543No Hit
GACTCTGGGCCCGCGCTCCGGCGTTCCGCGGATCCTCCTAGCCGTAAGGCCGCGTCGGTTTCGTCTAGCACGCGA2720.14482490562421133No Hit
GTCGAAGCTCGTTCGCGCGAGCGGACTCTGGGCCCGCGCTCCGGCGTTCCGCGGATCCTCCTAGCCGTAAGGCCG2690.14322757210629722No Hit
GGTTTGGAGGGGAAGCGGATGCGGCCGGTGCCGGGCCTGGTCGAAGCTCGTTCGCGCGAGCGGACTCTGGGCCCG2680.14269512760032585No Hit
GCCTCTAGTCGATAGAATAATGTAGGTAAGGGAAGTCGGCAAATTGGATCCGTAACTTCGGAATAAGGATTGGCT2670.14216268309435448No Hit
TCCTAGCCGTAAGGCCGCGTCGGTTTCGTCTAGCACGCGACTGGCGCGGTTCTGTACGACCGCCGTTCAACGGTC2640.1405653495764404No Hit
ATGTAGGTAAGGGAAGTCGGCAAATTGGATCCGTAACTTCGGAATAAGGATTGGCTCTGAGGACCGGGGCGCGTC2620.13950046056449766No Hit
CCGTAAGGCCGCGTCGGTTTCGTCTAGCACGCGACTGGCGCGGTTCTGTACGACCGCCGTTCAACGGTCAGCTCA2540.13524090451672674No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC2520.13417601550478403Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG2420.12885157044507037Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC2380.1267217924211849Clontech Universal Primer Mix Long (96% over 26bp)
GTCGGATTCCCCTTGTCCGTGCCAGTTCTGAGCTGACCGTTGAACGGCGGTCGTACAGAACCGCGCCAGTCGCGT2340.12459201439729946No Hit
GAACCGCGCCAGTCGCGTGCTAGACGAAACCGACGCGGCCTTACGGCTAGGAGGATCCGCGGAACGCCGGAGCGC2340.12459201439729946No Hit
CCTCAGAGCCAATCCTTATTCCGAAGTTACGGATCCAATTTGCCGACTTCCCTTACCTACATTATTCTATCGACT2320.1235271253853567No Hit
CCCAGAGTCCGCTCGCGCGAACGAGCTTCGACCAGGCCCGGCACCGGCCGCATCCGCTTCCCCTCCAAACCCGAC2270.12086490285549989No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT2230.11873512483161443Clontech Universal Primer Mix Long (96% over 26bp)
AGCGGACTCTGGGCCCGCGCTCCGGCGTTCCGCGGATCCTCCTAGCCGTAAGGCCGCGTCGGTTTCGTCTAGCAC2220.11820268032564306No Hit
GTTTTAATTAGACAGTCGGATTCCCCTTGTCCGTGCCAGTTCTGAGCTGACCGTTGAACGGCGGTCGTACAGAAC2200.11713779131370032No Hit
CCGTTGAACGGCGGTCGTACAGAACCGCGCCAGTCGCGTGCTAGACGAAACCGACGCGGCCTTACGGCTAGGAGG2190.11660534680772897No Hit
GTTCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAAGAGCCTCTAGTCGATAGAATAATGTAGGTAAGGGAAGT2140.11394312427787213No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT2060.10968356823010121Clontech SMART CDS Primer II A (96% over 26bp)
GTCCAGTACCGGCGACGCATCTTTCAAATGTCTGCCTTATCAACTTTCGATGGTAGTTTCTGCGACTACCATGGT2040.10861867921815849No Hit
GGTAAGGGAAGTCGGCAAATTGGATCCGTAACTTCGGAATAAGGATTGGCTCTGAGGACCGGGGCGCGTCGGGTT2000.10648890119427303No Hit
GGATTGGCTCTGAGGACCGGGGCGCGTCGGGTTTGGAGGGGAAGCGGATGCGGCCGGTGCCGGGCCTGGTCGAAG2000.10648890119427303No Hit
GTCCGTGCCAGTTCTGAGCTGACCGTTGAACGGCGGTCGTACAGAACCGCGCCAGTCGCGTGCTAGACGAAACCG1980.10542401218233029No Hit
ATCACATTGCGTCAACACCCGCGAGGGCCATCGCAATGCTTTGTTTTAATTAGACAGTCGGATTCCCCTTGTCCG1950.10382667866441621No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1940.10329423415844485No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCT1920.1022293451465021Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1910.10169690064053075Clontech Universal Primer Mix Long (96% over 26bp)
GCTTTGTTTTAATTAGACAGTCGGATTCCCCTTGTCCGTGCCAGTTCTGAGCTGACCGTTGAACGGCGGTCGTAC1890.10063201162858801No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)