FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002150389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187813
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG18220.9701138898798274Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG16570.882260546394552Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11950.6362711846357814No Hit
GTGCCGGGCCTGGTCGAAGCTCGTTCGCGCGAGCGGACTCTGGGCCCGCGCTCCGGCGTTCCGCGGATCCTCCTA11890.6330765175999531No Hit
GTACAGAACCGCGCCAGTCGCGTGCTAGACGAAACCGACGCGGCCTTACGGCTAGGAGGATCCGCGGAACGCCGG11750.625622294516354No Hit
CCCCTTGTCCGTGCCAGTTCTGAGCTGACCGTTGAACGGCGGTCGTACAGAACCGCGCCAGTCGCGTGCTAGACG9970.530847172453451No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT9080.48345961142199956Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCTGAGCTGACCGTTGAACGGCGGTCGTACAGAACCGCGCCAGTCGCGTGCTAGACGAAACCGACGCGGCCTT8550.45524005260551714No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC8020.42702049378903484Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCCGCGAGGGCCATCGCAATGCTTTGTTTTA7020.37377604319189833No Hit
CTCTGGGCCCGCGCTCCGGCGTTCCGCGGATCCTCCTAGCCGTAAGGCCGCGTCGGTTTCGTCTAGCACGCGACT6860.3652569310963565No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6120.32585603765447546No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG6090.3242587041365613Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCCGCGAGGGCCATCGCAATGCTTTGTTTTAATTAGA5260.28006581014093807No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5040.26835203100956806No Hit
TGCCAGTTCTGAGCTGACCGTTGAACGGCGGTCGTACAGAACCGCGCCAGTCGCGTGCTAGACGAAACCGACGCG4990.2656898084797112No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT4880.2598329189140262Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA4710.25078136231251297Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG4670.2486515842886275Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC4550.24226225021697112Clontech Universal Primer Mix Long (96% over 26bp)
GGCCCAGAGTCCGCTCGCGCGAACGAGCTTCGACCAGGCCCGGCACCGGCCGCATCCGCTTCCCCTCCAAACCCG4520.24066491669905704No Hit
GCCTTACGGCTAGGAGGATCCGCGGAACGCCGGAGCGCGGGCCCAGAGTCCGCTCGCGCGAACGAGCTTCGACCA4520.24066491669905704No Hit
GCTCTGAGGACCGGGGCGCGTCGGGTTTGGAGGGGAAGCGGATGCGGCCGGTGCCGGGCCTGGTCGAAGCTCGTT4490.23906758318114293No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG4060.21617246942437426Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGCAGAAATCACATTGCGTCAACACCCGCGAGGGCCATCGCAATGCTTTGTTTTAATTAGACAGTCGG4040.2151075804124315No Hit
AAATCACATTGCGTCAACACCCGCGAGGGCCATCGCAATGCTTTGTTTTAATTAGACAGTCGGATTCCCCTTGTC3860.20552357930494694No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC3680.19593957819746236Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCTGTACGACCGCCGTTCAACGGTCAGCTCAGAACTGGCACGGACAAGGGGAATCCGACTGTCTAATTAAAAC3660.19487468918551965No Hit
TAATTAGACAGTCGGATTCCCCTTGTCCGTGCCAGTTCTGAGCTGACCGTTGAACGGCGGTCGTACAGAACCGCG3640.1938098001735769No Hit
GTCGTACAGAACCGCGCCAGTCGCGTGCTAGACGAAACCGACGCGGCCTTACGGCTAGGAGGATCCGCGGAACGC3610.1922124666556628No Hit
GGCTAGGAGGATCCGCGGAACGCCGGAGCGCGGGCCCAGAGTCCGCTCGCGCGAACGAGCTTCGACCAGGCCCGG3530.18795291060789188No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3500.1863555770899778Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAATAATGTAGGTAAGGGAAGTCGGCAAATTGGATCCGTAACTTCGGAATAAGGATTGGCTCTGAGGACCGGGGC3490.18582313258400643No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCCGCGAGGGCCATCGCAATGCTTTGTTTTAA3340.17783646499443595No Hit
GACTCTGGGCCCGCGCTCCGGCGTTCCGCGGATCCTCCTAGCCGTAAGGCCGCGTCGGTTTCGTCTAGCACGCGA3260.17357690894666503No Hit
CTCCTAGCCGTAAGGCCGCGTCGGTTTCGTCTAGCACGCGACTGGCGCGGTTCTGTACGACCGCCGTTCAACGGT3040.161863129815295No Hit
GGCCCGGCACCGGCCGCATCCGCTTCCCCTCCAAACCCGACGCGCCCCGGTCCTCAGAGCCAATCCTTATTCCGA3020.16079824080335228No Hit
GGCCATCGCAATGCTTTGTTTTAATTAGACAGTCGGATTCCCCTTGTCCGTGCCAGTTCTGAGCTGACCGTTGAA2950.1570711292615527No Hit
GCGTCAACACCCGCGAGGGCCATCGCAATGCTTTGTTTTAATTAGACAGTCGGATTCCCCTTGTCCGTGCCAGTT2930.15600624024961No Hit
TCGCAATGCTTTGTTTTAATTAGACAGTCGGATTCCCCTTGTCCGTGCCAGTTCTGAGCTGACCGTTGAACGGCG2930.15600624024961No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2910.15494135123766725No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCCGCGAGGGCCATCGCAATGCTTTGTTTT2840.1512142396958677No Hit
GTCGAAGCTCGTTCGCGCGAGCGGACTCTGGGCCCGCGCTCCGGCGTTCCGCGGATCCTCCTAGCCGTAAGGCCG2820.15014935068392496No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC2760.14695468364809677Clontech Universal Primer Mix Long (96% over 26bp)
TCCTAGCCGTAAGGCCGCGTCGGTTTCGTCTAGCACGCGACTGGCGCGGTTCTGTACGACCGCCGTTCAACGGTC2730.14535735013018267No Hit
CGGCTAGGAGGATCCGCGGAACGCCGGAGCGCGGGCCCAGAGTCCGCTCGCGCGAACGAGCTTCGACCAGGCCCG2660.14163023858838314No Hit
GCCTCTAGTCGATAGAATAATGTAGGTAAGGGAAGTCGGCAAATTGGATCCGTAACTTCGGAATAAGGATTGGCT2650.14109779408241174No Hit
ATGTAGGTAAGGGAAGTCGGCAAATTGGATCCGTAACTTCGGAATAAGGATTGGCTCTGAGGACCGGGGCGCGTC2570.13683823803464085No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC2490.13257868198686992Clontech Universal Primer Mix Long (96% over 26bp)
GGTTTGGAGGGGAAGCGGATGCGGCCGGTGCCGGGCCTGGTCGAAGCTCGTTCGCGCGAGCGGACTCTGGGCCCG2390.12725423692715626No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG2370.12618934791521352Clontech Universal Primer Mix Long (96% over 25bp)
CCGTAAGGCCGCGTCGGTTTCGTCTAGCACGCGACTGGCGCGGTTCTGTACGACCGCCGTTCAACGGTCAGCTCA2260.12033245834952852No Hit
CCCAGAGTCCGCTCGCGCGAACGAGCTTCGACCAGGCCCGGCACCGGCCGCATCCGCTTCCCCTCCAAACCCGAC2240.11926756933758578No Hit
GTCGGATTCCCCTTGTCCGTGCCAGTTCTGAGCTGACCGTTGAACGGCGGTCGTACAGAACCGCGCCAGTCGCGT2170.11554045779578624No Hit
GTTTTAATTAGACAGTCGGATTCCCCTTGTCCGTGCCAGTTCTGAGCTGACCGTTGAACGGCGGTCGTACAGAAC2120.11287823526592941No Hit
GTTCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAAGAGCCTCTAGTCGATAGAATAATGTAGGTAAGGGAAGT2100.11181334625398667No Hit
GTCCGTGCCAGTTCTGAGCTGACCGTTGAACGGCGGTCGTACAGAACCGCGCCAGTCGCGTGCTAGACGAAACCG2070.11021601273607257No Hit
AGCGGACTCTGGGCCCGCGCTCCGGCGTTCCGCGGATCCTCCTAGCCGTAAGGCCGCGTCGGTTTCGTCTAGCAC2040.10861867921815849No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCT2030.10808623471218713Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTCAGAGCCAATCCTTATTCCGAAGTTACGGATCCAATTTGCCGACTTCCCTTACCTACATTATTCTATCGACT1970.10489156767635893No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC1920.1022293451465021Clontech Universal Primer Mix Long (96% over 26bp)
ATCACATTGCGTCAACACCCGCGAGGGCCATCGCAATGCTTTGTTTTAATTAGACAGTCGGATTCCCCTTGTCCG1900.10116445613455938No Hit
GCTTTGTTTTAATTAGACAGTCGGATTCCCCTTGTCCGTGCCAGTTCTGAGCTGACCGTTGAACGGCGGTCGTAC1900.10116445613455938No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)