FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002150402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246049
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG28431.1554609041288524Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG24310.988014582461217Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT13280.5397298912005333Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCTATACTCGACCGTCGGAGCAATTGCCTGGCTCCGATGAGTAGGAGGACGCGGGGGTCGCTGAGAAACCTCGG12950.5263179285426887No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10130.41170661128474406Clontech Universal Primer Mix Long (96% over 26bp)
AGTATAGGTTGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCT9770.39707537929436815No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG8890.361310145540116Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC8180.332454104670208Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG8160.3316412584485204Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA8050.3271706042292389Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7370.29953383269186223No Hit
CCCCTAGGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGACTTCCCTTACCTACA7050.2865282931448614No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATT7040.2861218700340176No Hit
GTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCCAACGCACCCAA6090.24751167450385897No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG6020.24466671272795254Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC5900.23978963539782724Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT5760.2340997118460144Clontech Universal Primer Mix Long (96% over 26bp)
GTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCTCCACCAGGTGCTGGAAGG5690.23125475007010798No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC5570.2263776727399827Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG5500.22353271096407626Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG5200.21134001763876303Clontech Universal Primer Mix Long (96% over 25bp)
CCCTTGATACAAGGCTTCCCCATCTCTTAGGATCGACTAACCCATGTCCAAGTGCTGTTCACATGGAACCTTTCC5030.20443082475441882No Hit
TTCTACGCCCGTAGCCCAATCGGCACGCGACTGCCCGCCCTCGCCGCGAGAGCAGCTCGGGCAGACTGCCGACAG4980.20239870920019995No Hit
GTACTCGGTCCTCCAGATTTTCAAGGGCCGCCGAGGGCGCACCGGACACCGCAAGACGTGCGGTGCTCTTCCAGC4970.20199228608935615No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT4640.1885803234315116Clontech SMART CDS Primer II A (96% over 26bp)
AGCTTGGGTGGATCGGCCCCCGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGACTGA4610.1873610540989803No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4540.18451609232307387No Hit
ACCCAAGCTCGCGCCCGAGGTTTCTCAGCGACCCCCGCGTCCTCCTACTCATCGGAGCCAGGCAATTGCTCCGAC4520.1837032461013863No Hit
TACCTACATTGTTCTATTGACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCATGA4470.18167113054716744No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT4300.17476193766282327No Hit
CTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCTTAGCGGATTTCGACT4270.17354266833029194No Hit
GTGCACCCTTGATACAAGGCTTCCCCATCTCTTAGGATCGACTAACCCATGTCCAAGTGCTGTTCACATGGAACC4230.17191697588691682No Hit
CCTTTGTGCTGTCCGGTGCGCTCTCGACGACCCTTGAAAATCCGCCGGAAGGAATGATTTTCACGCGAGGTCGTA4150.16866559100016662No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC4130.16785274477847908Clontech Universal Primer Mix Long (96% over 26bp)
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTTG4100.16663347544594775No Hit
GCGCTGAAGCGCGCAACCTATACTCGACCGTCGGAGCAATTGCCTGGCTCCGATGAGTAGGAGGACGCGGGGGTC4060.16500778300257266No Hit
CTGCACGAGCATCGAAAGGGAATCGGGTTAATATTCCCGAACCGGGACATGGCGGCGGGCGGCAACGTTAGGAAA4040.1641949367808851No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC3950.16053712878329113No Hit
CTCGTGCAGAGTGCACCCTTGATACAAGGCTTCCCCATCTCTTAGGATCGACTAACCCATGTCCAAGTGCTGTTC3870.15728574389654093No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTT3790.15403435900979073No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC3750.15240866656641563Clontech Universal Primer Mix Long (96% over 26bp)
ATCCTAAGCCATAGGGAAACTCCGTTTTAAAGTGCGCTCTCGGGCGCCGCCTGGCGAAAGGGAAACCGGTTAATA3690.149970127901353No Hit
AGGGTGCACTCTGCACGAGCATCGAAAGGGAATCGGGTTAATATTCCCGAACCGGGACATGGCGGCGGGCGGCAA3640.1479380123471341No Hit
GCTCAGGCATAGTTCACCATCTTTCGGGTCCCAACAGGTGTGCTCGCACTCGAACCCTTCGCATAAGCTCAAGGT3620.14712516612544654No Hit
AACCTAGATCCCACAAAGGGTGTTGGTTGATTAAGACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGG3560.1446866274603839No Hit
ATCCTAAGAGATGGGGAAGCCTTGTATCAAGGGTGCACTCTGCACGAGCATCGAAAGGGAATCGGGTTAATATTC3490.14184166568447748No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGA3410.13859028079772728No Hit
CCGCAAGACGTGCGGTGCTCTTCCAGCTGCTGGACCCTATCTCCGGGTGAACCGATTCCAGGGTGGGCAAGCTGT3410.13859028079772728No Hit
ATTCCAGGGTGGGCAAGCTGTTAAAAAGAAAAGATAACTCTTCCCGGGGCCCCCGACGACGTCTCCGGATTTCCT3390.13777743457603972No Hit
TCCTCCTACTCATCGGAGCCAGGCAATTGCTCCGACGGTCGAGTATAGGTTGCGCGCTTCAGCGCCATCCATTTT3320.13493247280013332No Hit
GTCTAACATGTGTGCGAGTCGGCGGGTTTGAAAACCTGCGAGGCGCAAGGAAGCTGACGGGCGAGATCCCCTCTC3280.13330678035675822No Hit
ACCGAGTACCGTTCATGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCAATAGA3220.13086824169169556No Hit
GGTGCACACGGCCCGCCACGAGGGCGCACGCTTCGTTCCTCGACCACCGCAGCGGCAAACCGACAGGCAGTAATC3160.12842970302663292No Hit
GTTCTGAGTCGACTGTTCGACGCCTAGGGAAAAGCCCCCGGCAGGGGGCATTCTACGCCCGTAGCCCAATCGGCA3160.12842970302663292No Hit
GTATCAAGGGTGCACTCTGCACGAGCATCGAAAGGGAATCGGGTTAATATTCCCGAACCGGGACATGGCGGCGGG3110.12639758747241403No Hit
ATCTAGGTTAGCGCGCAATTCGGCACCGTAACTCGACTTTCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAA3090.12558474125072647No Hit
GAGTACGACCGGGCATGAACGGTACTCGGTCCTCCAGATTTTCAAGGGCCGCCGAGGGCGCACCGGACACCGCAA2990.12152051014228872No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCTCCACCAGG2970.12070766392060117No Hit
GTCTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTAATGGCAGGTAGAGCAGCACACCTTTGTGCTGTCC2970.12070766392060117No Hit
GCACAAAGGTGTGCTGCTCTACCTGCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGATAGACAGTTA2960.1203012408097574No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC2900.11786270214469476Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2890.11745627903385097No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTGAAGCGCGCAACCTA2860.11623700970131966No Hit
CCCTGGAATCGGTTCACCCGGAGATAGGGTCCAGCAGCTGGAAGAGCACCGCACGTCTTGCGGTGTCCGGTGCGC2860.11623700970131966No Hit
GCTCAGGCTGGTCCGCCGCTCGACTTGAGGTGCAAACCAACACGGGCTGCTGCATCGTCACAAGCGCGGGCGGAT2850.11583058659047588No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTGAAGCGCGCAACCTATACTCGACCG2840.11542416347963211No Hit
TGCCTGAGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCTCGTAGCGATACTGACGTGCAAATCGTTCGTCTG2840.11542416347963211No Hit
ACCTTGAGCTTATGCGAAGGGTTCGAGTGCGAGCACACCTGTTGGGACCCGAAAGATGGTGAACTATGCCTGAGC2830.11501774036878833No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCAACCAACACCCTTTGTGGGATCTAGG2800.11379847103625701No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCTCC2800.11379847103625701No Hit
CCCGTAGCCCAATCGGCACGCGACTGCCCGCCCTCGCCGCGAGAGCAGCTCGGGCAGACTGCCGACAGCCCACGA2780.11298562481456946No Hit
CTGTAGAAGCGTCCTCAGCGGCGGACCTGGGCCAAGTCCCTTGGAAGGGGGCGCCTGAGAGGGTGACAGCCCCGT2780.11298562481456946No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGAAAATCATTCCTTCCGGCGG2750.11176635548203813No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA2750.11176635548203813No Hit
GTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCTCCACCAGGTGCTGGAAGGCGCCGGCCGTG2750.11176635548203813No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG2690.10932781681697548Clontech Universal Primer Mix Long (96% over 26bp)
GTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTGAAGCGCGCAACCTATACTCGAC2640.10729570126275662No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG2640.10729570126275662Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCCAGCACCTGGTGGAGGACGCTCTTGGCAGGGTTCGCCCGTCCGGCGCACGCTTAACAACCAACTTAGAACTG2620.10648285504106907No Hit
CTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTACG2600.10567000881938152No Hit
CCCTCTTACCTATTTAAAGTTTGAGAATAGGTCGAGGGCGTTACGCCCCCGATGCCTCTAATCATTTGCTTTACC2560.10404431637600642No Hit
GTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCCAACGCACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGT2550.10363789326516264No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTA2550.10363789326516264No Hit
ATGTAGGTAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCCTAGGG2520.10241862393263129No Hit
ATCTAGTAGCTGGTTTCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCACGCGCGAGTTTTATCGGGTAAAGCA2500.10160577771094374No Hit
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCAATAGAACAATGTAGGTAAGGGAAGTC2490.10119935460009997No Hit
ATACAAGGCTTCCCCATCTCTTAGGATCGACTAACCCATGTCCAAGTGCTGTTCACATGGAACCTTTCCCCACTT2480.1007929314892562No Hit
GTGATAGACAGTTAAGAAGAAAAGAGAACTCTTCCCGGGGCCCCCGCCGACGTCTCCACCTTCAGTTGCGTTGCC2480.1007929314892562No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGACTGAAGTGGGGAAAGGTTCCATGTGA2470.10038650837841243No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)