FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002150403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246049
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG21590.8774674963117103Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG17070.693764250210324Clontech Universal Primer Mix Long (96% over 26bp)
ACCTATACTCGACCGTCGGAGCAATTGCCTGGCTCCGATGAGTAGGAGGACGCGGGGGTCGCTGAGAAACCTCGG10400.42268003527752607No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT9100.36984503086783527Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTATAGGTTGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCT8630.35074314465817785No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC7370.29953383269186223Clontech Universal Primer Mix Long (96% over 26bp)
CTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATT6910.28083836959304853No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG6790.27596129226292326Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA6520.2649878682701413Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC6340.2576722522749534Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG5510.22393913407492005Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCCAACGCACCCAA5450.2215005954098574No Hit
CCCCTAGGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGACTTCCCTTACCTACA5030.20443082475441882No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG4540.18451609232307387Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC4470.18167113054716744Clontech Universal Primer Mix Long (96% over 26bp)
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTTG4330.17598120699535458No Hit
GTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCTCCACCAGGTGCTGGAAGG4210.17110412966522928No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT4200.1706977065543855Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC4120.1674463216676353Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG4070.16541420611341642Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG4000.16256924433751Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3950.16053712878329113No Hit
GCGCTGAAGCGCGCAACCTATACTCGACCGTCGGAGCAATTGCCTGGCTCCGATGAGTAGGAGGACGCGGGGGTC3840.1560664745640096No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT3650.14834443545797787No Hit
AACCTAGATCCCACAAAGGGTGTTGGTTGATTAAGACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGG3610.14671874301460278No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT3600.146312319903759Clontech SMART CDS Primer II A (96% over 26bp)
TTCTACGCCCGTAGCCCAATCGGCACGCGACTGCCCGCCCTCGCCGCGAGAGCAGCTCGGGCAGACTGCCGACAG3550.14428020434954011No Hit
CTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCTTAGCGGATTTCGACT3510.14265451190616502No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC3490.14184166568447748No Hit
ACCCAAGCTCGCGCCCGAGGTTTCTCAGCGACCCCCGCGTCCTCCTACTCATCGGAGCCAGGCAATTGCTCCGAC3420.13899670390857105No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTT3300.13411962657844576No Hit
GTACTCGGTCCTCCAGATTTTCAAGGGCCGCCGAGGGCGCACCGGACACCGCAAGACGTGCGGTGCTCTTCCAGC3300.13411962657844576No Hit
CTGCACGAGCATCGAAAGGGAATCGGGTTAATATTCCCGAACCGGGACATGGCGGCGGGCGGCAACGTTAGGAAA3290.133713203467602No Hit
AGGGTGCACTCTGCACGAGCATCGAAAGGGAATCGGGTTAATATTCCCGAACCGGGACATGGCGGCGGGCGGCAA3160.12842970302663292No Hit
CCTTTGTGCTGTCCGGTGCGCTCTCGACGACCCTTGAAAATCCGCCGGAAGGAATGATTTTCACGCGAGGTCGTA3150.12802327991578913No Hit
TACCTACATTGTTCTATTGACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCATGA3020.12273977947482005No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC2970.12070766392060117Clontech Universal Primer Mix Long (96% over 26bp)
ATCCTAAGCCATAGGGAAACTCCGTTTTAAAGTGCGCTCTCGGGCGCCGCCTGGCGAAAGGGAAACCGGTTAATA2930.11908197147722607No Hit
GTCTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTAATGGCAGGTAGAGCAGCACACCTTTGTGCTGTCC2860.11623700970131966No Hit
ACCGAGTACCGTTCATGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCAATAGA2840.11542416347963211No Hit
GTCTAACATGTGTGCGAGTCGGCGGGTTTGAAAACCTGCGAGGCGCAAGGAAGCTGACGGGCGAGATCCCCTCTC2820.11461131725794456No Hit
GCTCAGGCATAGTTCACCATCTTTCGGGTCCCAACAGGTGTGCTCGCACTCGAACCCTTCGCATAAGCTCAAGGT2790.11339204792541323No Hit
CCCTTGATACAAGGCTTCCCCATCTCTTAGGATCGACTAACCCATGTCCAAGTGCTGTTCACATGGAACCTTTCC2770.11257920170372568No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGA2670.10851497059528793No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC2650.10770212437360038Clontech Universal Primer Mix Long (96% over 26bp)
ATCCTAAGAGATGGGGAAGCCTTGTATCAAGGGTGCACTCTGCACGAGCATCGAAAGGGAATCGGGTTAATATTC2600.10567000881938152No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAG2600.10567000881938152No Hit
GCTCAGGCTGGTCCGCCGCTCGACTTGAGGTGCAAACCAACACGGGCTGCTGCATCGTCACAAGCGCGGGCGGAT2560.10404431637600642No Hit
GTATCAAGGGTGCACTCTGCACGAGCATCGAAAGGGAATCGGGTTAATATTCCCGAACCGGGACATGGCGGCGGG2550.10363789326516264No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTGAAGCGCGCAACCTA2530.10282504704347507No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCTCC2520.10241862393263129No Hit
ATCTAGGTTAGCGCGCAATTCGGCACCGTAACTCGACTTTCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAA2490.10119935460009997No Hit
TTCCAGCACCTGGTGGAGGACGCTCTTGGCAGGGTTCGCCCGTCCGGCGCACGCTTAACAACCAACTTAGAACTG2470.10038650837841243No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)