FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002150406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229381
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTATACTCGGCCATGGGCGCAAGTGCGAAGCTCCCATGAGTAGGAGGGCGTGGGTGTCGTGGCGCAGCCTGCG23251.0135974644804933No Hit
AGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCT19820.8640645912259515No Hit
CCCCTAGGCCCAACCCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCATTTTGCCGACTTCCCTTATCTACA10610.4625492085220659No Hit
ATACAGGATTGCCCCATCTCTTAGGATCGACTAACCCATGTCCAATTGCTGTTCACATGGAACCTTTCTCCACTT9450.4119783242727166No Hit
ATCCTGTATCAAGGGCGCGATTCGCGCCGCCCATCGAAAGGGAAAAGGGTTAAGATTCCCTTACCTGGACAAGGC9080.39584795602076894No Hit
GCGCTGAAGCGCGCGACCTATACTCGGCCATGGGCGCAAGTGCGAAGCTCCCATGAGTAGGAGGGCGTGGGTGTC8740.38102545546492517No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCTGGCAAGATCGGAAATGCTCCCCCGGA7780.33917368918960156No Hit
GATATAGACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCA7730.33699390969609516No Hit
CTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCTTAGCGGATTTCGACT7570.33001861531687454No Hit
GTCTATATCAACCAACACCCTTTGTGGGATCTAGGTTAGCGCGCAGTTTGGCACCTTAACTCGGCTTTCGGATCA7120.3104005998753166No Hit
ATCCTAAGAGATGGGGCAATCCTGTATCAAGGGCGCGATTCGCGCCGCCCATCGAAAGGGAAAAGGGTTAAGATT6460.28162751056103164No Hit
TATCTACATTGTTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCTGGCAAGA6150.2681128777012917No Hit
GTCCAGGTAAGGGAATCTTAACCCTTTTCCCTTTCGATGGGCGGCGCGAATCGCGCCCTTGATACAGGATTGCCC5960.25982971562596724No Hit
GCTCAGGCATAGTTCACCATCTTTCGGGTCCCAACAGGTATGCTCGTACTCAAACCTTTCACAGAAGATCATGGT5960.25982971562596724No Hit
GAGTAGGAGGGCGTGGGTGTCGTGGCGCAGCCTGCGGCGTGAGCCGGGGTGAAACGGCACCTAGTGCAGATCTTG5910.25764993613246084No Hit
TACCTGGACAAGGCGGCTGACGGCAACGTAAGCGAGCCCGGAGACGCCGGCGGTGGCCCTGGGAAGAGTTCTCTT5790.2524184653480454No Hit
GTGCCAAACTGCGCGCTAACCTAGATCCCACAAAGGGTGTTGGTTGATATAGACAGCAGGACGGTGGTCATGGAA5700.2484948622597338No Hit
GTTCTGAGTTGGTTGTTCGTCGCCTAGGGAGCGGCGTGAGCCTTTCTCCGCCCGTCCGCCGGCGGCACGCGGCCG5640.2458791268675261No Hit
GTGCTTTACCAGCTGCTGAGCCCTATCTCCGGATGATCCGATTCCAGGGCCTTCGAGCTGTTAAAAAGAAAAGAG5550.24195552377921448No Hit
GTGTCAACATCCTTTGGGACCATCACAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTT5440.2371600088935003No Hit
ATCTCCGGATGATCCGATTCCAGGGCCTTCGAGCTGTTAAAAAGAAAAGAGAACTCTTCCCAGGGCCACCGCCGG5360.23367236170389002No Hit
CTCCTACTCATGGGAGCTTCGCACTTGCGCCCATGGCCGAGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCG5240.2284408909194746No Hit
AACCTAGATCCCACAAAGGGTGTTGGTTGATATAGACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGG5200.2266970673246694No Hit
GTATGTGCTGGGTGCTGGTCAGCATCGGTTGGCGGGCCGGGAGAAAAGTGGTCTTTGATCGGCCGCCCATGCCGC5120.22320942013505915No Hit
TGCCTGAGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCTCGTAGATGTGCTGACGTGCAAATCGCTTTTCGG5070.22102964064155273No Hit
CCCCTTGTCCGTACCAGTTCTGAGTTGGTTGTTCGTCGCCTAGGGAGCGGCGTGAGCCTTTCTCCGCCCGTCCGC5010.21841390524934495No Hit
CTCTTAGGATCGACTAACCCATGTCCAATTGCTGTTCACATGGAACCTTTCTCCACTTCAGTCTTCAAAGTTCTC4970.21667008165453983No Hit
GCCTCTAGTCGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGATTGGCT4830.2105666990727218No Hit
ATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCCCAGGATAGCTGGAGCTCGGTCAGTTTTATCGGGTAAAGCGAAT4500.19618015441557934No Hit
CTCAATGAGAGCTCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGATCCGAAAGCCGAGTTAA4360.1900767718337613No Hit
TCCGGAGATAGGGCTCAGCAGCTGGTAAAGCACCGCACGTCTCGCGGTGTCCGGCGCGCCACCGACGGTCCTTGA4350.18964081593506No Hit
TCCCAGGGCCACCGCCGGCGTCTCCGGGCTCGCTTACGTTGCCGTCAGCCGCCTTGTCCAGGTAAGGGAATCTTA4190.18266552155583943No Hit
ATGTAGATAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGTTGGGCCTAGGG4120.17961383026493039No Hit
CTCATTGAGTGCCTGGGTTCAATTAAGCAACCCAGACGTCTTACCTATTTAAAGTTTGAGAATAGGTCGAGAATG4030.1756902271766188No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTGAAGCGCGCGACCTATACTCGGCCA4020.17525427127791754No Hit
TCACAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTTGGTTGTTCGTCGCCT3940.17176662408830723No Hit
CCGCGAGACGTGCGGTGCTTTACCAGCTGCTGAGCCCTATCTCCGGATGATCCGATTCCAGGGCCTTCGAGCTGT3800.1656632415064892No Hit
CCTTTGGGACCATCACAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTTGGT3790.1652272856077879No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTGAAGCGCGCGACCTA3770.16435537381038534No Hit
ATACTCGGCCATGGGCGCAAGTGCGAAGCTCCCATGAGTAGGAGGGCGTGGGTGTCGTGGCGCAGCCTGCGGCGT3760.16391941791168405No Hit
GCCTAGGGGTCTGCGCCCGGAAACCGGCGGCTGTCGGACGGCTGCCCGAGCTGCTCACGCGGCGAGGGCGGACGG3750.16348346201298275No Hit
GTATCAAGGGCGCGATTCGCGCCGCCCATCGAAAGGGAAAAGGGTTAAGATTCCCTTACCTGGACAAGGCGGCTG3700.16130368251947633No Hit
TCTTAGGATCGACTAACCCATGTCCAATTGCTGTTCACATGGAACCTTTCTCCACTTCAGTCTTCAAAGTTCTCA3680.16043177072207376No Hit
ACCCAGACGTCTTACCTATTTAAAGTTTGAGAATAGGTCGAGAATGTTTCATCCCCGGTACCTCTAATCATTCGC3630.1582519912285673No Hit
CTATAGAAGCGACCTCTGTGTCGGACCGGGCCCAAGTTCCCTGGAAAGGGACGTCGCAGAGGGTGAGAACCCCGT3590.15650816763376216No Hit
GAGTACGACCTGGCAAGATCGGAAATGCTCCCCCGGATTTTCAAGGACCGTCGGTGGCGCGCCGGACACCGCGAG3590.15650816763376216No Hit
CCCTTGATACAGGATTGCCCCATCTCTTAGGATCGACTAACCCATGTCCAATTGCTGTTCACATGGAACCTTTCT3570.1556362558363596No Hit
GATTAGAGGTACCGGGGATGAAACATTCTCGACCTATTCTCAAACTTTAAATAGGTAAGACGTCTGGGTTGCTTA3540.15432838814025573No Hit
GTCCGTACCAGTTCTGAGTTGGTTGTTCGTCGCCTAGGGAGCGGCGTGAGCCTTTCTCCGCCCGTCCGCCGGCGG3480.15171265274804802No Hit
ATGCCTGAGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCTCGTAGATGTGCTGACGTGCAAATCGCTTTTCG3480.15171265274804802No Hit
CTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCCGAAAAGCGATTTGCACGTCAGCACATCTACG3480.15171265274804802No Hit
GCTCGGGCAGCCGTCCGACAGCCGCCGGTTTCCGGGCGCAGACCCCTAGGCCCAACCCTCAGAGCCAATCCTTTT3390.14778904965973644No Hit
GTTTGGCACCTTAACTCGGCTTTCGGATCATCCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTTGGAGC3370.14691713786233385No Hit
ATCTAGGTTAGCGCGCAGTTTGGCACCTTAACTCGGCTTTCGGATCATCCCGCATCGCCAGTTCTGCTTACCAAA3360.14648118196363255No Hit
GCTTAGGGCTGCATTCCCAAACAACCCGACTCTGCGAAAGCGTCTCGTGGAGTGTCCGCAACCGGAGCCGACGGG3360.14648118196363255No Hit
GTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTGAAGCGCGCGACCTATACTCGGC3350.14604522606493128No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTATATCAACCAACACCCTTTGTGGGATCTAGG3280.14299353477402227No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCCCAGGATAGCTGGAG3250.14168566707791838No Hit
GTCTAACATGTATGCGAGTTGGTGGGTGGAAAACCCATGAAGCGCAAGTAACCTGACTGGTGGGAGGGCTTGTGC3210.13994184348311323No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACATCCTTTGGGACCATCACAATGCTTTGTTTTA3130.13645419629350294No Hit
GTGTCGGACCGGGCCCAAGTTCCCTGGAAAGGGACGTCGCAGAGGGTGAGAACCCCGTCGGCTCCGGTTGCGGAC3130.13645419629350294No Hit
GTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTATATCAACCAACACCCTTTGTGG3100.13514632859739908No Hit
GGGTTAGTCGATCCTAAGAGATGGGGCAATCCTGTATCAAGGGCGCGATTCGCGCCGCCCATCGAAAGGGAAAAG3090.13471037269869782No Hit
CTCGTGGAGTGTCCGCAACCGGAGCCGACGGGGTTCTCACCCTCTGCGACGTCCCTTTCCAGGGAACTTGGGCCC3060.13340250500259393No Hit
TTCAAGGACCGTCGGTGGCGCGCCGGACACCGCGAGACGTGCGGTGCTTTACCAGCTGCTGAGCCCTATCTCCGG3040.13253059320519137No Hit
GGGCAATCCTGTATCAAGGGCGCGATTCGCGCCGCCCATCGAAAGGGAAAAGGGTTAAGATTCCCTTACCTGGAC3020.13165868140778877No Hit
GGATTGCCCCATCTCTTAGGATCGACTAACCCATGTCCAATTGCTGTTCACATGGAACCTTTCTCCACTTCAGTC3000.1307867696103862No Hit
GCGCCGGACACCGCGAGACGTGCGGTGCTTTACCAGCTGCTGAGCCCTATCTCCGGATGATCCGATTCCAGGGCC3000.1307867696103862No Hit
GTTATGAGTACGACCTGGCAAGATCGGAAATGCTCCCCCGGATTTTCAAGGACCGTCGGTGGCGCGCCGGACACC2930.1277350783194772No Hit
TTCTCCGCCCGTCCGCCGGCGGCACGCGGCCGTCCGCCCTCGCCGCGTGAGCAGCTCGGGCAGCCGTCCGACAGC2890.12599125472467207No Hit
CGTCTGGGTTGCTTAATTGAACCCAGGCACTCAATGAGAGCTCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGC2850.1242474311298669No Hit
CGCTTACGTTGCCGTCAGCCGCCTTGTCCAGGTAAGGGAATCTTAACCCTTTTCCCTTTCGATGGGCGGCGCGAA2790.12163169573765917No Hit
GCCCTGGGAAGAGTTCTCTTTTCTTTTTAACAGCTCGAAGGCCCTGGAATCGGATCATCCGGAGATAGGGCTCAG2740.11945191624415274No Hit
GTGCTGGTCAGCATCGGTTGGCGGGCCGGGAGAAAAGTGGTCTTTGATCGGCCGCCCATGCCGCCCCGTCGACCG2740.11945191624415274No Hit
ACGTAAGCGAGCCCGGAGACGCCGGCGGTGGCCCTGGGAAGAGTTCTCTTTTCTTTTTAACAGCTCGAAGGCCCT2720.11858000444675015No Hit
GTACTTGTTCGCTATCGGTCTCCCGCCAGTATTTAGCCTTAGATGGGATTTACCACCTGCTTAGGGCTGCATTCC2720.11858000444675015No Hit
CCTCTAATCATTCGCTTTACCCGATAAAACTGACCGAGCTCCAGCTATCCTGGGGGAAACTTCGGAGGGAACCAG2710.11814404854804889No Hit
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTAGTCGATAGAACAATGTAGATAAGGGAAGTC2700.11770809264934759No Hit
GTCCGGCGCGCCACCGACGGTCCTTGAAAATCCGGGGGAGCATTTCCGATCTTGCCAGGTCGTACTCATAACCGC2690.11727213675064631No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGACTGAAGTGGAGAAAGGTTCCATGTGA2680.11683618085194501No Hit
GATCTGCACTAGGTGCCGTTTCACCCCGGCTCACGCCGCAGGCTGCGCCACGACACCCACGCCCTCCTACTCATG2670.11640022495324374No Hit
ATCACATTGTGTCAACATCCTTTGGGACCATCACAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCG2670.11640022495324374No Hit
GTACATGGGTTCGACCTGAGATCAGACAAGACTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAA2630.11465640135843859No Hit
GCCCTAAGCAGGTGGTAAATCCCATCTAAGGCTAAATACTGGCGGGAGACCGATAGCGAACAAGTACCGTGAGGG2570.11204066596623086No Hit
CCTTGGAGACCTGATGCGGTTATGAGTACGACCTGGCAAGATCGGAAATGCTCCCCCGGATTTTCAAGGACCGTC2550.1111687541688283No Hit
CTCCACGAGACGCTTTCGCAGAGTCGGGTTGTTTGGGAATGCAGCCCTAAGCAGGTGGTAAATCCCATCTAAGGC2500.10898897467532184No Hit
GCCGAGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACAC2480.10811706287791928No Hit
CCTATACCCAAGTCCGAAAAGCGATTTGCACGTCAGCACATCTACGAGCCTCCACCAGAGTTTCCTCTGGCTTCG2470.10768110697921798No Hit
GTTCTCACCCTCTGCGACGTCCCTTTCCAGGGAACTTGGGCCCGGTCCGACACAGAGGTCGCTTCTATAGACTAC2470.10768110697921798No Hit
ACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTAGTCGATAGAACAATGTAGATAAGGGAAGTCGGCAA2470.10768110697921798No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2450.10680919518181539No Hit
GTCCTTGAAAATCCGGGGGAGCATTTCCGATCTTGCCAGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAA2440.10637323928311412No Hit
GTGCGGGCACAAGCCCTCCCACCAGTCAGGTTACTTGCGCTTCATGGGTTTTCCACCCACCAACTCGCATACATG2440.10637323928311412No Hit
CGCCTAGGGAGCGGCGTGAGCCTTTCTCCGCCCGTCCGCCGGCGGCACGCGGCCGTCCGCCCTCGCCGCGTGAGC2440.10637323928311412No Hit
AAATCACATTGTGTCAACATCCTTTGGGACCATCACAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTC2430.10593728338441283No Hit
GTTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCTGGCAAGATCGGAAATGC2420.10550132748571155No Hit
CCCATGAGTAGGAGGGCGTGGGTGTCGTGGCGCAGCCTGCGGCGTGAGCCGGGGTGAAACGGCACCTAGTGCAGA2400.10462941568830898No Hit
GCCCTATCTCCGGATGATCCGATTCCAGGGCCTTCGAGCTGTTAAAAAGAAAAGAGAACTCTTCCCAGGGCCACC2360.10288559209350383No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACATCCTTTGGGACCATCACAATGCTTTGTTTTAATTAAA2360.10288559209350383No Hit
CTTTGGGACCATCACAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTTGGTT2350.10244963619480253No Hit
GACTAACCCATGTCCAATTGCTGTTCACATGGAACCTTTCTCCACTTCAGTCTTCAAAGTTCTCATTTGAATATT2330.10157772439739997No Hit
GCGTGAGCCTTTCTCCGCCCGTCCGCCGGCGGCACGCGGCCGTCCGCCCTCGCCGCGTGAGCAGCTCGGGCAGCC2310.10070581259999738No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)