FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002150407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229381
Sequences flagged as poor quality0
Sequence length75
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTATACTCGGCCATGGGCGCAAGTGCGAAGCTCCCATGAGTAGGAGGGCGTGGGTGTCGTGGCGCAGCCTGCG22220.9686940069142607No Hit
AGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCT17150.7476643662727078No Hit
CCCCTAGGCCCAACCCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCATTTTGCCGACTTCCCTTATCTACA8050.35094449845453635No Hit
GCGCTGAAGCGCGCGACCTATACTCGGCCATGGGCGCAAGTGCGAAGCTCCCATGAGTAGGAGGGCGTGGGTGTC7710.33612199789869257No Hit
ATCCTGTATCAAGGGCGCGATTCGCGCCGCCCATCGAAAGGGAAAAGGGTTAAGATTCCCTTACCTGGACAAGGC7490.32653096812726423No Hit
GATATAGACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCA6750.294270231623369No Hit
ATACAGGATTGCCCCATCTCTTAGGATCGACTAACCCATGTCCAATTGCTGTTCACATGGAACCTTTCTCCACTT6620.28860280494025226No Hit
CTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCTTAGCGGATTTCGACT6090.265497142309084No Hit
ATCCTAAGAGATGGGGCAATCCTGTATCAAGGGCGCGATTCGCGCCGCCCATCGAAAGGGAAAAGGGTTAAGATT5850.2550342007402531No Hit
GCTCAGGCATAGTTCACCATCTTTCGGGTCCCAACAGGTATGCTCGTACTCAAACCTTTCACAGAAGATCATGGT5370.23410831760259132No Hit
GTCTATATCAACCAACACCCTTTGTGGGATCTAGGTTAGCGCGCAGTTTGGCACCTTAACTCGGCTTTCGGATCA5200.2266970673246694No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCTGGCAAGATCGGAAATGCTCCCCCGGA5110.22277346423635785No Hit
GTGCCAAACTGCGCGCTAACCTAGATCCCACAAAGGGTGTTGGTTGATATAGACAGCAGGACGGTGGTCATGGAA5100.2223375083376566No Hit
GCCTCTAGTCGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGATTGGCT4690.2044633164909038No Hit
GAGTAGGAGGGCGTGGGTGTCGTGGCGCAGCCTGCGGCGTGAGCCGGGGTGAAACGGCACCTAGTGCAGATCTTG4630.20184758109869605No Hit
TACCTGGACAAGGCGGCTGACGGCAACGTAAGCGAGCCCGGAGACGCCGGCGGTGGCCCTGGGAAGAGTTCTCTT4540.19792397801038447No Hit
GTTCTGAGTTGGTTGTTCGTCGCCTAGGGAGCGGCGTGAGCCTTTCTCCGCCCGTCCGCCGGCGGCACGCGGCCG4510.1966161103142806No Hit
AACCTAGATCCCACAAAGGGTGTTGGTTGATATAGACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGG4500.19618015441557934No Hit
CTCCTACTCATGGGAGCTTCGCACTTGCGCCCATGGCCGAGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCG4280.186589124644151No Hit
ATGTAGATAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGTTGGGCCTAGGG4270.1861531687454497No Hit
TATCTACATTGTTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCTGGCAAGA4230.18440934515064455No Hit
GTATGTGCTGGGTGCTGGTCAGCATCGGTTGGCGGGCCGGGAGAAAAGTGGTCTTTGATCGGCCGCCCATGCCGC4150.18092169796103427No Hit
GTCCAGGTAAGGGAATCTTAACCCTTTTCCCTTTCGATGGGCGGCGCGAATCGCGCCCTTGATACAGGATTGCCC4120.17961383026493039No Hit
GTGTCAACATCCTTTGGGACCATCACAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTT4080.17787000667012523No Hit
TCCGGAGATAGGGCTCAGCAGCTGGTAAAGCACCGCACGTCTCGCGGTGTCCGGCGCGCCACCGACGGTCCTTGA3850.16784302099999565No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTGAAGCGCGCGACCTATACTCGGCCA3660.1595598589246712No Hit
TCACAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTTGGTTGTTCGTCGCCT3640.1586879471272686No Hit
GTGCTTTACCAGCTGCTGAGCCCTATCTCCGGATGATCCGATTCCAGGGCCTTCGAGCTGTTAAAAAGAAAAGAG3560.1552002999376583No Hit
CCTTTGGGACCATCACAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTTGGT3490.15214860864674928No Hit
CCCCTTGTCCGTACCAGTTCTGAGTTGGTTGTTCGTCGCCTAGGGAGCGGCGTGAGCCTTTCTCCGCCCGTCCGC3480.15171265274804802No Hit
CTCTTAGGATCGACTAACCCATGTCCAATTGCTGTTCACATGGAACCTTTCTCCACTTCAGTCTTCAAAGTTCTC3480.15171265274804802No Hit
ATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCCCAGGATAGCTGGAGCTCGGTCAGTTTTATCGGGTAAAGCGAAT3480.15171265274804802No Hit
TGCCTGAGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCTCGTAGATGTGCTGACGTGCAAATCGCTTTTCGG3450.15040478505194416No Hit
GCCTAGGGGTCTGCGCCCGGAAACCGGCGGCTGTCGGACGGCTGCCCGAGCTGCTCACGCGGCGAGGGCGGACGG3400.1482250055584377No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTGAAGCGCGCGACCTA3390.14778904965973644No Hit
CTCAATGAGAGCTCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGATCCGAAAGCCGAGTTAA3370.14691713786233385No Hit
GATTAGAGGTACCGGGGATGAAACATTCTCGACCTATTCTCAAACTTTAAATAGGTAAGACGTCTGGGTTGCTTA3340.14560927016623No Hit
ATACTCGGCCATGGGCGCAAGTGCGAAGCTCCCATGAGTAGGAGGGCGTGGGTGTCGTGGCGCAGCCTGCGGCGT3270.14255757887532097No Hit
GTTTGGCACCTTAACTCGGCTTTCGGATCATCCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTTGGAGC3240.1412497111792171No Hit
GTATCAAGGGCGCGATTCGCGCCGCCCATCGAAAGGGAAAAGGGTTAAGATTCCCTTACCTGGACAAGGCGGCTG3160.13776206398960683No Hit
CTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCCGAAAAGCGATTTGCACGTCAGCACATCTACG3160.13776206398960683No Hit
ATCTCCGGATGATCCGATTCCAGGGCCTTCGAGCTGTTAAAAAGAAAAGAGAACTCTTCCCAGGGCCACCGCCGG3150.13732610809090554No Hit
GTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTGAAGCGCGCGACCTATACTCGGC3110.13558228449610038No Hit
GTACTTGTTCGCTATCGGTCTCCCGCCAGTATTTAGCCTTAGATGGGATTTACCACCTGCTTAGGGCTGCATTCC3070.13383846090129523No Hit
CTCATTGAGTGCCTGGGTTCAATTAAGCAACCCAGACGTCTTACCTATTTAAAGTTTGAGAATAGGTCGAGAATG3060.13340250500259393No Hit
GCTTAGGGCTGCATTCCCAAACAACCCGACTCTGCGAAAGCGTCTCGTGGAGTGTCCGCAACCGGAGCCGACGGG3050.13296654910389266No Hit
GTCCGTACCAGTTCTGAGTTGGTTGTTCGTCGCCTAGGGAGCGGCGTGAGCCTTTCTCCGCCCGTCCGCCGGCGG2950.12860699011687976No Hit
ACCCAGACGTCTTACCTATTTAAAGTTTGAGAATAGGTCGAGAATGTTTCATCCCCGGTACCTCTAATCATTCGC2930.1277350783194772No Hit
ATCTAGGTTAGCGCGCAGTTTGGCACCTTAACTCGGCTTTCGGATCATCCCGCATCGCCAGTTCTGCTTACCAAA2920.1272991224207759No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCCCAGGATAGCTGGAG2920.1272991224207759No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTATATCAACCAACACCCTTTGTGGGATCTAGG2880.12555529882597077No Hit
TCCCAGGGCCACCGCCGGCGTCTCCGGGCTCGCTTACGTTGCCGTCAGCCGCCTTGTCCAGGTAAGGGAATCTTA2880.12555529882597077No Hit
ATGCCTGAGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCTCGTAGATGTGCTGACGTGCAAATCGCTTTTCG2840.12381147523116562No Hit
CGTCTGGGTTGCTTAATTGAACCCAGGCACTCAATGAGAGCTCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGC2830.12337551933246432No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACATCCTTTGGGACCATCACAATGCTTTGTTTTA2770.1207597839402566No Hit
GTCTAACATGTATGCGAGTTGGTGGGTGGAAAACCCATGAAGCGCAAGTAACCTGACTGGTGGGAGGGCTTGTGC2750.11988787214285404No Hit
CTATAGAAGCGACCTCTGTGTCGGACCGGGCCCAAGTTCCCTGGAAAGGGACGTCGCAGAGGGTGAGAACCCCGT2750.11988787214285404No Hit
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTAGTCGATAGAACAATGTAGATAAGGGAAGTC2740.11945191624415274No Hit
TCTTAGGATCGACTAACCCATGTCCAATTGCTGTTCACATGGAACCTTTCTCCACTTCAGTCTTCAAAGTTCTCA2740.11945191624415274No Hit
GTTATGAGTACGACCTGGCAAGATCGGAAATGCTCCCCCGGATTTTCAAGGACCGTCGGTGGCGCGCCGGACACC2700.11770809264934759No Hit
GAGTACGACCTGGCAAGATCGGAAATGCTCCCCCGGATTTTCAAGGACCGTCGGTGGCGCGCCGGACACCGCGAG2640.11509235725713986No Hit
CCGCGAGACGTGCGGTGCTTTACCAGCTGCTGAGCCCTATCTCCGGATGATCCGATTCCAGGGCCTTCGAGCTGT2610.113784489561036No Hit
GTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTATATCAACCAACACCCTTTGTGG2590.11291257776363343No Hit
GCCCTGGGAAGAGTTCTCTTTTCTTTTTAACAGCTCGAAGGCCCTGGAATCGGATCATCCGGAGATAGGGCTCAG2570.11204066596623086No Hit
GTGTCGGACCGGGCCCAAGTTCCCTGGAAAGGGACGTCGCAGAGGGTGAGAACCCCGTCGGCTCCGGTTGCGGAC2510.10942493057402314No Hit
GCCCTAAGCAGGTGGTAAATCCCATCTAAGGCTAAATACTGGCGGGAGACCGATAGCGAACAAGTACCGTGAGGG2470.10768110697921798No Hit
GCCGAGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACAC2430.10593728338441283No Hit
GGGCAATCCTGTATCAAGGGCGCGATTCGCGCCGCCCATCGAAAGGGAAAAGGGTTAAGATTCCCTTACCTGGAC2380.10375750389090639No Hit
GTCCGGCGCGCCACCGACGGTCCTTGAAAATCCGGGGGAGCATTTCCGATCTTGCCAGGTCGTACTCATAACCGC2370.10332154799220512No Hit
ATCACATTGTGTCAACATCCTTTGGGACCATCACAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCG2350.10244963619480253No Hit
GGGTTAGTCGATCCTAAGAGATGGGGCAATCCTGTATCAAGGGCGCGATTCGCGCCGCCCATCGAAAGGGAAAAG2350.10244963619480253No Hit
CTCGTGGAGTGTCCGCAACCGGAGCCGACGGGGTTCTCACCCTCTGCGACGTCCCTTTCCAGGGAACTTGGGCCC2320.10114176849869867No Hit
GTACATGGGTTCGACCTGAGATCAGACAAGACTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAA2310.10070581259999738No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)