FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002150428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252530
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11330.4486595652001742No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6280.24868332475349464No Hit
ATGTAGATAAGGGAAGTCGGCAAATTAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCATCG4090.1619609551340435No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4040.1599809923573437No Hit
GTCTACCACCCTGAAATCGGATTATCCGGAGCTAGGGTTCAATGGCTGGTAGAGCTCGGCACTTCCGTCGAGTCC3920.1552290816932642No Hit
GTGGTAGACTGTTAAGGAGAAAAGATAACTCTTCCCGGGGCCCTTGCCAGCGTCTCCAAGTTCATTTACGTTGCC3370.1334494911495664No Hit
GTTCTAAGTTGGTTGTTAAGCGTACACCAGACGTCCGAAGACAGCCAAGGCCTTCCACAAGCCAGTCCGGACAGA3370.1334494911495664No Hit
GTCAAGAACGCACCGGACTCGACGGAAGTGCCGAGCTCTACCAGCCATTGAACCCTAGCTCCGGATAATCCGATT3340.1322615134835465No Hit
CAGTAGTCCCATCCAGTCCGGCCGCTTCCAACCAAGGCCCGATGCACCCAACCCTTAGAGCCAATCCTTTTCCCG3230.12790559537480695No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGCGCAA3030.11998574426800776No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGCGCAAAAATTATTCATTCCCTTGG3000.11879776660198788No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2780.11008593038450878No Hit
GTTCTTGACAGTCCTTGAAAATCCAAGGGAATGAATAATTTTTGCGCCTGGTCGTACTCATAACCGCAGCAGGTC2570.10177008672236962No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)