FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002150429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252530
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6010.2379915257593157No Hit
ATGTAGATAAGGGAAGTCGGCAAATTAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCATCG3890.1540411040272443No Hit
GTCTACCACCCTGAAATCGGATTATCCGGAGCTAGGGTTCAATGGCTGGTAGAGCTCGGCACTTCCGTCGAGTCC3760.1488932008078248No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3680.14572526036510514No Hit
GTGGTAGACTGTTAAGGAGAAAAGATAACTCTTCCCGGGGCCCTTGCCAGCGTCTCCAAGTTCATTTACGTTGCC3410.13503346137092623No Hit
GTCAAGAACGCACCGGACTCGACGGAAGTGCCGAGCTCTACCAGCCATTGAACCCTAGCTCCGGATAATCCGATT3360.1330534985942264No Hit
GTTCTAAGTTGGTTGTTAAGCGTACACCAGACGTCCGAAGACAGCCAAGGCCTTCCACAAGCCAGTCCGGACAGA3230.12790559537480695No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGCGCAA3180.12592563259810716No Hit
CAGTAGTCCCATCCAGTCCGGCCGCTTCCAACCAAGGCCCGATGCACCCAACCCTTAGAGCCAATCCTTTTCCCG3030.11998574426800776No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAATTAGATCCGTAACTTCGGGAAAAGGATTGGCT2740.10850196016314895No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGCGCAAAAATTATTCATTCCCTTGG2720.10770997505246901No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)