FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002150434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322703
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18110.5611971379255848No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12490.38704319451631997No Hit
ATGTAGGCAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCACGGGG12280.3805356628230912No Hit
TTCCCGGTCCGTCCCCCGGCCGGCACGCGGCGACCCGCTCTCGCCGCGAGAGCAGCTCGAGCAGTCCGCCGACAG9830.30461445973542234No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6700.20762124926015563No Hit
CCCCTTGTCCGTACCAGTTCTGAGTCGGCTGTTCGACGCCCGGGGAAGGCCCCCGAGGGGGCCGTTCCCGGTCCG6550.20297301233642082No Hit
GTCGGCGGACTGCTCGAGCTGCTCTCGCGGCGAGAGCGGGTCGCCGCGTGCCGGCCGGGGGACGGACCGGGAACG6210.19243700864262187No Hit
GTTCTGAGTCGGCTGTTCGACGCCCGGGGAAGGCCCCCGAGGGGGCCGTTCCCGGTCCGTCCCCCGGCCGGCACG6020.18654924187255775No Hit
ATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGAATGGGCACGGGG5220.161758644945972No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTGAGCATCCGCGGGGACCATCGCAATGCTTTGTTTTA5170.1602092326380604No Hit
GTCCGCCGACAGCCGACGGGTTCGGGGCCGGGACCCCCGTGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAAGTT5140.1592795852533134No Hit
AAATCACATTGCGTGAGCATCCGCGGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTC5050.1564906430990725No Hit
GTCCGTACCAGTTCTGAGTCGGCTGTTCGACGCCCGGGGAAGGCCCCCGAGGGGGCCGTTCCCGGTCCGTCCCCC4960.15370170094483163No Hit
CCCTGGAAACGGCTCAGCCGGAGGTAGGGTCCAGCGGTCGGAAGAGCGCCGCACGTCGCGCGGCGTCCGGTGCGC4930.15277205356008466No Hit
TGCCTACATTGTTCCATGGGCCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGCGG4860.1506028763290084No Hit
GACCGGGAACGGCCCCCTCGGGGGCCTTCCCCGGGCGTCGAACAGCCGACTCAGAACTGGTACGGACAAGGGGAA4780.14812381663634983No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4590.1422360498662857No Hit
TACCAGTTCTGAGTCGGCTGTTCGACGCCCGGGGAAGGCCCCCGAGGGGGCCGTTCCCGGTCCGTCCCCCGGCCG4530.1403767550967918No Hit
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCCCATGGAACAATGTAGGCAAGGGAAGTC4510.13975699017362714No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTGAGCATCCGCGGGGACCATCGCAATGCTTTGTTTTAATTAAA4420.13696804801938625No Hit
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGGCTGTTCGACGCCC4330.13417910586514534No Hit
GTACATGGGCAGAAATCACATTGCGTGAGCATCCGCGGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGG4310.13355934094198071No Hit
GCCCCGAACCCGTCGGCTGTCGGCGGACTGCTCGAGCTGCTCTCGCGGCGAGAGCGGGTCGCCGCGTGCCGGCCG4130.1279814566334989No Hit
GCTCTGAGGGCTGGGCACGGGGGTCCCGGCCCCGAACCCGTCGGCTGTCGGCGGACTGCTCGAGCTGCTCTCGCG4000.12395298463292873No Hit
GAGTACGACCGGGCGCGGGCGGCACTCGGTCCTCCGGATTTTCAAGGGCCGCCGGGGGCGCACCGGACGCCGCGC3850.11930474770919391No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTGAGCATCCGCGGGGACCATCGCAATGCTTTGTTTTAA3720.11527627570862371No Hit
CCTCTGGCCCATGGAACAATGTAGGCAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAAAAGGATTGGCTC3610.11186756863121818No Hit
GAACAATGTAGGCAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCA3590.11124780370805354No Hit
ACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCCCATGGAACAATGTAGGCAAGGGAAGTCGGCAA3340.10350074216849549No Hit
ATCACATTGCGTGAGCATCCGCGGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCG3320.10288097724533085No Hit
GGCCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGCGGGCGGCACTCGGTCCTCCG3320.10288097724533085No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)