FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002150454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422279
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCATGGACCTTGAAAATCTATGAAAGGGATACGCAAATTCTCAATAGCCTGTACCGAATCCGCAGCAGGTCTC30060.7118516431079926No Hit
GTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAAACCGTGCCAGTT28360.6715938988204482No Hit
CCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGATGGCCTTTCATAGACGACACGTGAATGCCATCCATTA27750.6571484729290351No Hit
CTCATAGAGAAGCCGTTGGGAGGCATCGGAAGAGTTTTCTTTTCTGTTTTACAATCGTACGCACCATGGAAGTCT24880.5891839281612393No Hit
ACCCAATACTTATCCAATCCTTAGAGCCAATTCTTTTTCCGAAGTTACGAATCTATTTTGCCGACTTCCCTTATC23530.5572145429917188No Hit
AGCCTAGAGACATTAAAATGAATCCTGGGAAGAGTTATCTTTTCTATTTAACATAGTACCTGACCTTTGAAACAG23330.5524783377814194No Hit
TCCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGATGGCCTTTCATAGACGACACGTGAATGCCATCCATT22080.5228770552170484No Hit
TTCATAGACGACACGTGAATGCCATCCATTACAGACTTAATATACATCGCACCGCGAAGCACAATGCATATTGTA19780.46841069529860596No Hit
CTTAATATACATCGCACCGCGAAGCACAATGCATATTGTAAAGCCCGAGCCACCACAGTCTTCAGAGCCAATCCT18300.4333627767423907No Hit
GTATAACAGATTGGTAGAGCATGACATTAAATTGTTGTGTCGGTGCCCTCTCCATGGACCTTGAAAATCTATGAA16070.3805540886475529No Hit
AGCCACCACAGTCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATT15750.37297616031107395No Hit
ATGTAGGTAAGGGAAGTCGGCAAATTAGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGACTGTGGTGGCTC15590.36918719614283446No Hit
GCCTCTAGTTGATAGAATAATGTAGATAAGGGAAGTCGGCAAAATAGATTCGTAACTTCGGAAAAAGAATTGGCT14700.3481110829570024No Hit
GTGTCAACATTATCACTAACCTTCACAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTT14250.3374546212338288No Hit
AGCCTGTACCGAATCCGCAGCAGGTCTCCAAGATTAAGAGTCTCTAGTCGATAGAACAATGTAGGTAAGGGAAGT14140.3348497083681642No Hit
TACCTACATTGTTCTATCGACTAGAGACTCTTAATCTTGGAGACCTGCTGCGGATTCGGTACAGGCTATTGAGAA13930.3298766928973499No Hit
ACTAGAGGCTGTTCACCTTGAGGACCTGCTGCGGTTATGGGTACGACTTAGTGTAAAATTTTATAAAAGCTCCTC13690.32419324664499066No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTT13450.3185098003926314No Hit
CTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTGTTCTATCGACT12740.30169627189606874No Hit
TGGTATAACAGATTGGTAGAGCATGACATTAAATTGTTGTGTCGGTGCCCTCTCCATGGACCTTGAAAATCTATG11880.2813305894917815No Hit
CGTACGCACCATGGAAGTCTTTCATAGAGAGATATGGTATAACAGATTGGTAGAGCATGACATTAAATTGTTGTG11730.27777843558405696No Hit
GTTCTAAGTCGATTGTTAAACATAAGTTGAATATAAATTAACAAGAAGACACGACAACATCTCTAGTTTAGTAAA11630.2754103329789073No Hit
GTACCTGACCTTTGAAACAGTTTAACTGGAGATAAGGTTGGGGAGGTGGCAGAGCAGCACTTTTTAAGTGAGGTC11200.2652274917767637No Hit
AATATACATCGCACCGCGAAGCACAATGCATATTGTAAAGCCCGAGCCACCACAGTCTTCAGAGCCAATCCTTAT10860.25717594291925483No Hit
GTTCTGAATTGACTGTTAATTGATGGCCTTTCATAGACGACACGTGAATGCCATCCATTACAGACTTAATATACA10750.25457103005359016No Hit
GTATTGGGTATTTATGTACTTTTTGATGTGATGATGTTGATGGTTTAGTTTACTAAACTAGAGATGTTGTCGTGT10590.2507820658853507No Hit
GTCGTACCCATAACCGCAGCAGGTCCTCAAGGTGAACAGCCTCTAGTTGATAGAATAATGTAGATAAGGGAAGTC10300.2439145683304166No Hit
ACATAGTACCTGACCTTTGAAACAGTTTAACTGGAGATAAGGTTGGGGAGGTGGCAGAGCAGCACTTTTTAAGTG10250.2427305170278418No Hit
TAATTAGACAGTCGGATTCCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGATGGCCTTTCATAGACGACA10200.24154646572526695No Hit
GCCTAGAGACATTAAAATGAATCCTGGGAAGAGTTATCTTTTCTATTTAACATAGTACCTGACCTTTGAAACAGT10190.24130965546475197No Hit
ATCACATTGTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAAACCG9860.23349491686775803No Hit
GTACATGGGCAGAAATCACATTGTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTTAATTAGACAGTCG9270.21952311149737494No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTTAATTAG9260.21928630123686No Hit
GTCTAGCAAAGAGCGAAAGGGAATATGGTTCCAATTCCATAACCTATTGAGTATACGTTTGTCATTGGTCTCCAC8770.20768259847162657No Hit
GTCCATGGAGAGGGCACCGACACAACAATTTAATGTCATGCTCTACCAATCTGTTATACCATATCTCTCTATGAA8610.2038936343033871No Hit
AAATCACATTGTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAAAC8470.20057829065617755No Hit
ATTTAACATAGTACCTGACCTTTGAAACAGTTTAACTGGAGATAAGGTTGGGGAGGTGGCAGAGCAGCACTTTTT8400.19892061883257278No Hit
CACCATGGAAGTCTTTCATAGAGAGATATGGTATAACAGATTGGTAGAGCATGACATTAAATTGTTGTGTCGGTG8230.19489484440381832No Hit
ATCCATTACAGACTTAATATACATCGCACCGCGAAGCACAATGCATATTGTAAAGCCCGAGCCACCACAGTCTTC7990.18921139815145913No Hit
TTCATAGAGAGATATGGTATAACAGATTGGTAGAGCATGACATTAAATTGTTGTGTCGGTGCCCTCTCCATGGAC7850.18589605450424956No Hit
TTGTAAAGCCCGAGCCACCACAGTCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCC7820.18518562372270467No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACATTATCACTAACCTTCACAATGCTATGTTTTA7720.18281752111755498No Hit
GTATAACAGCTGTAATTTCTTAGATCATATTCCCAGTACTTGGATAACTGTGGAAAATCTAGAGCTAATACATGC7550.17879174668880052No Hit
GTGGTGGCTCGGGCTTTACAATATGCATTGTGCTTCGCGGTGCGATGTATATTAAGTCTGTAATGGATGGCATTC7550.17879174668880052No Hit
ACCGAGAACCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAAACCGTGCCAGTTCTGAATTGACTG7450.17642364408365085No Hit
GTTAATTGATGGCCTTTCATAGACGACACGTGAATGCCATCCATTACAGACTTAATATACATCGCACCGCGAAGC7210.17074019783129163No Hit
GTACAGGCTATTGAGAATTTGCGTATCCCTTTCATAGATTTTCAAGGTCCATGGAGAGGGCACCGACACAACAAT7120.1686089054866569No Hit
GCATATTGTAAAGCCCGAGCCACCACAGTCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGA7110.16837209522614197No Hit
TCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGATGG7030.16647761314202222No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTTA7000.16576718236047733No Hit
GAGTATACGTTTGTCATTGGTCTCCACGCAAGTGGATTAAAGGCTCTTGACATCTCGGTAACGAGGAGCTCTCAT6830.16174140793172287No Hit
ACATTATTCTATCAACTAGAGGCTGTTCACCTTGAGGACCTGCTGCGGTTATGGGTACGACTTAGTGTAAAATTT6810.16126778741069292No Hit
GTATACGTTTGTCATTGGTCTCCACGCAAGTGGATTAAAGGCTCTTGACATCTCGGTAACGAGGAGCTCTCATAG6770.16032054636863305No Hit
GCGAAGCACAATGCATATTGTAAAGCCCGAGCCACCACAGTCTTCAGAGCCAATCCTTATCCCGAAGTTACGGAT6710.15889968480554326No Hit
ATACTTATCCAATCCTTAGAGCCAATTCTTTTTCCGAAGTTACGAATCTATTTTGCCGACTTCCCTTATCTACAT6690.1584260642845133No Hit
TGCCGGGAGTGGGTAATTTACGCGCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA6450.1527426180321541No Hit
CGTGAATGCCATCCATTACAGACTTAATATACATCGCACCGCGAAGCACAATGCATATTGTAAAGCCCGAGCCAC6400.15155856672957926No Hit
GATTGGTAGAGCATGACATTAAATTGTTGTGTCGGTGCCCTCTCCATGGACCTTGAAAATCTATGAAAGGGATAC6270.1484800333428847No Hit
ACATCGCACCGCGAAGCACAATGCATATTGTAAAGCCCGAGCCACCACAGTCTTCAGAGCCAATCCTTATCCCGA6260.1482432230823697No Hit
GAATAATGTAGATAAGGGAAGTCGGCAAAATAGATTCGTAACTTCGGAAAAAGAATTGGCTCTAAGGATTGGATA6140.1454014999561901No Hit
TCCTCAAGGTGAACAGCCTCTAGTTGATAGAATAATGTAGATAAGGGAAGTCGGCAAAATAGATTCGTAACTTCG6090.14421744865361524No Hit
CCATGGAAGTCTTTCATAGAGAGATATGGTATAACAGATTGGTAGAGCATGACATTAAATTGTTGTGTCGGTGCC6080.1439806383931003No Hit
CTCAATAGCCTGTACCGAATCCGCAGCAGGTCTCCAAGATTAAGAGTCTCTAGTCGATAGAACAATGTAGGTAAG6060.14350701787207037No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACATTATCACTAACCTTCACAATGCTATGTTTTAATTAGA6010.1423229665694955No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACCCGTTGCAACCA5990.14184934604846558No Hit
GTCCGTACCAGTTCTAAGTCGATTGTTAAACATAAGTTGAATATAAATTAACAAGAAGACACGACAACATCTCTA5980.14161253578795063No Hit
GCACAATGCATATTGTAAAGCCCGAGCCACCACAGTCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCTAAT5980.14161253578795063No Hit
GCTCTGAAGACTGTGGTGGCTCGGGCTTTACAATATGCATTGTGCTTCGCGGTGCGATGTATATTAAGTCTGTAA5900.1397180537038309No Hit
TTCCATAACCTATTGAGTATACGTTTGTCATTGGTCTCCACGCAAGTGGATTAAAGGCTCTTGACATCTCGGTAA5890.13948124344331592No Hit
CCCCTTGTCCGTACCAGTTCTAAGTCGATTGTTAAACATAAGTTGAATATAAATTAACAAGAAGACACGACAACA5890.13948124344331592No Hit
GATTAAGAGTCTCTAGTCGATAGAACAATGTAGGTAAGGGAAGTCGGCAAATTAGATCCGTAACTTCGGGATAAG5860.13877081266177102No Hit
ATCACATTGTGTCAACATTATCACTAACCTTCACAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCG5850.13853400240125605No Hit
GTTTTAATTAGACAGTCGGATTCCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGATGGCCTTTCATAGAC5770.1366395203171363No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATTCGTAACTTCGGAAAAAGAATTGGCTCTAAGGATTGGATAAGTAT5760.13640271005662133No Hit
ACCCATAACCGCAGCAGGTCCTCAAGGTGAACAGCCTCTAGTTGATAGAATAATGTAGATAAGGGAAGTCGGCAA5680.1345082279725016No Hit
GTACATAAATACCCAATACTTATCCAATCCTTAGAGCCAATTCTTTTTCCGAAGTTACGAATCTATTTTGCCGAC5620.13308736640941177No Hit
CCATTACAGACTTAATATACATCGCACCGCGAAGCACAATGCATATTGTAAAGCCCGAGCCACCACAGTCTTCAG5580.1321401253673519No Hit
GTCTCTAGTCGATAGAACAATGTAGGTAAGGGAAGTCGGCAAATTAGATCCGTAACTTCGGGATAAGGATTGGCT5510.13048245354374716No Hit
CCTTAGAGCCAATTCTTTTTCCGAAGTTACGAATCTATTTTGCCGACTTCCCTTATCTACATTATTCTATCAACT5460.1292984022411723No Hit
GTATATTAAGTCTGTAATGGATGGCATTCACGTGTCGTCTATGAAAGGCCATCAATTAACAGTCAATTCAGAACT5390.12764073041756754No Hit
CCTTGAGGACCTGCTGCGGTTATGGGTACGACTTAGTGTAAAATTTTATAAAAGCTCCTCTAGATTTTCAAGGAT5390.12764073041756754No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5350.12669348937550767No Hit
TCACTAACCTTCACAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTCGATTG5330.12621986885447772No Hit
ATCGTACGCACCATGGAAGTCTTTCATAGAGAGATATGGTATAACAGATTGGTAGAGCATGACATTAAATTGTTG5300.12550943807293283No Hit
ACATTAAATTGTTGTGTCGGTGCCCTCTCCATGGACCTTGAAAATCTATGAAAGGGATACGCAAATTCTCAATAG5190.12290452520726818No Hit
CTCTCATAGAGAAGCCGTTGGGAGGCATCGGAAGAGTTTTCTTTTCTGTTTTACAATCGTACGCACCATGGAAGT5180.12266771494675321No Hit
GTACATGGGGATTTGACACTGAAAGGGAATACAGTTAATATTCTGTAAATTAGATGTAAAATAAGGCGGTAACGC5180.12266771494675321No Hit
GGGTACGACTTAGTGTAAAATTTTATAAAAGCTCCTCTAGATTTTCAAGGATTAAAATGAATGCGCCGGACCTCA5120.12124685338366342No Hit
GCCTTTCATAGACGACACGTGAATGCCATCCATTACAGACTTAATATACATCGCACCGCGAAGCACAATGCATAT5050.11958918156005863No Hit
GACTTAATATACATCGCACCGCGAAGCACAATGCATATTGTAAAGCCCGAGCCACCACAGTCTTCAGAGCCAATC5030.1191155610390287No Hit
GGTTGGGGAGGTGGCAGAGCAGCACTTTTTAAGTGAGGTCCGGCGCATTCATTTTAATCCTTGAAAATCTAGAGG5020.11887875077851373No Hit
GTATCAACGCAGAGTACATGGGGATTTGACACTGAAAGGGAATACAGTTAATATTCTGTAAATTAGATGTAAAAT4970.11769469947593889No Hit
TATCTACATTATTCTATCAACTAGAGGCTGTTCACCTTGAGGACCTGCTGCGGTTATGGGTACGACTTAGTGTAA4890.11580021739181914No Hit
CCCTTATCTACATTATTCTATCAACTAGAGGCTGTTCACCTTGAGGACCTGCTGCGGTTATGGGTACGACTTAGT4860.11508978661027426No Hit
ACACTAAGTCGTACCCATAACCGCAGCAGGTCCTCAAGGTGAACAGCCTCTAGTTGATAGAATAATGTAGATAAG4850.11485297634975927No Hit
CCTATTGAGTATACGTTTGTCATTGGTCTCCACGCAAGTGGATTAAAGGCTCTTGACATCTCGGTAACGAGGAGC4840.11461616608924431No Hit
GAACAATGTAGGTAAGGGAAGTCGGCAAATTAGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGACTGTGGT4820.11414254556821439No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTT4820.11414254556821439No Hit
AAATCACATTGTGTCAACATTATCACTAACCTTCACAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTC4790.11343211478666948No Hit
TACTTATCCAATCCTTAGAGCCAATTCTTTTTCCGAAGTTACGAATCTATTTTGCCGACTTCCCTTATCTACATT4780.11319530452615452No Hit
CCTCTATACTAACATCAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGCACTAGGCCATACGATCAGC4780.11319530452615452No Hit
GTCGGTGCCCTCTCCATGGACCTTGAAAATCTATGAAAGGGATACGCAAATTCTCAATAGCCTGTACCGAATCCG4770.11295849426563953No Hit
GTACATGGGCAGAAATCACATTGTGTCAACATTATCACTAACCTTCACAATGCTATGTTTTAATTAGACAGTCAG4760.11272168400512457No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTCGATTGTTAAACATAAGTTGAATATAAA4730.11201125322357969No Hit
GTTAATATTCTGTAAATTAGATGTAAAATAAGGCGGTAACGCGGCGAGCCTAGAGACATTAAAATGAATCCTGGG4730.11201125322357969No Hit
CAGTTAATATTCTGTAAATTAGATGTAAAATAAGGCGGTAACGCGGCGAGCCTAGAGACATTAAAATGAATCCTG4570.10822228905534019No Hit
CCCATAACCGCAGCAGGTCCTCAAGGTGAACAGCCTCTAGTTGATAGAATAATGTAGATAAGGGAAGTCGGCAAA4570.10822228905534019No Hit
GTCGGATTCCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGATGGCCTTTCATAGACGACACGTGAATGCC4510.10680142749225037No Hit
TACCAGTAATGTACGCTCGTCTCAAAGGTTAAGCCATGCATGTCTAAGTACAAACTAAAATAAAGTGAAACCGCA4500.10656461723173541No Hit
AAGTCGTACCCATAACCGCAGCAGGTCCTCAAGGTGAACAGCCTCTAGTTGATAGAATAATGTAGATAAGGGAAG4460.10561737618967554No Hit
GTTTTACAATCGTACGCACCATGGAAGTCTTTCATAGAGAGATATGGTATAACAGATTGGTAGAGCATGACATTA4400.10419651462658575No Hit
CCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGA4390.10395970436607078No Hit
CTCTTAATCTTGGAGACCTGCTGCGGATTCGGTACAGGCTATTGAGAATTTGCGTATCCCTTTCATAGATTTTCA4390.10395970436607078No Hit
CTGTTATACCATATCTCTCTATGAAAGACTTCCATGGTGCGTACGATTGTAAAACAGAAAAGAAAACTCTTCCGA4370.10348608384504084No Hit
GCACTAGGCCATACGATCAGCAAAGTTATCTAGAGTTCCACCATATAAAACAACCCGAAGGTCGATTGGTTTGTC4340.10277565306349593No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)