FastQCFastQC Report
Wed 8 May 2024
EGAF00002150455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422279
Total Bases31.6 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCATGGACCTTGAAAATCTATGAAAGGGATACGCAAATTCTCAATAGC31460.7450050795800881No Hit
CCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGATGGCCTTTCATA26000.6157066773389157No Hit
CTCATAGAGAAGCCGTTGGGAGGCATCGGAAGAGTTTTCTTTTCTGTTTT23030.5453740299659704No Hit
AGCCTAGAGACATTAAAATGAATCCTGGGAAGAGTTATCTTTTCTATTTA22160.5247715373011681No Hit
ACCCAATACTTATCCAATCCTTAGAGCCAATTCTTTTTCCGAAGTTACGA20930.4956438752578272No Hit
TCCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGATGGCCTTTCAT20270.48001439806383933No Hit
TTCATAGACGACACGTGAATGCCATCCATTACAGACTTAATATACATCGC20130.47669905441662974No Hit
CTTAATATACATCGCACCGCGAAGCACAATGCATATTGTAAAGCCCGAGC16030.379606847605493No Hit
ATGTAGGTAAGGGAAGTCGGCAAATTAGATCCGTAACTTCGGGATAAGGA15600.36942400640334944No Hit
GTGTCAACATTATCACTAACCTTCACAATGCTATGTTTTAATTAGACAGT15270.3616092678063555No Hit
GTATAACAGATTGGTAGAGCATGACATTAAATTGTTGTGTCGGTGCCCTC15230.36066202676429565No Hit
AGCCACCACAGTCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCTAA15210.3601884062432657No Hit
ACTAGAGGCTGTTCACCTTGAGGACCTGCTGCGGTTATGGGTACGACTTA15110.35782030363811607No Hit
AGCCTGTACCGAATCCGCAGCAGGTCTCCAAGATTAAGAGTCTCTAGTCG15030.3559258215539963No Hit
GTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTTAATTAGACAG14690.3478742726964874No Hit
TACCTACATTGTTCTATCGACTAGAGACTCTTAATCTTGGAGACCTGCTG14230.33698100071279885No Hit
CTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTC12560.2974336872067993No Hit
GTTCTAAGTCGATTGTTAAACATAAGTTGAATATAAATTAACAAGAAGAC12020.284645933138991No Hit
GCCTCTAGTTGATAGAATAATGTAGATAAGGGAAGTCGGCAAAATAGATT11710.27730481506302707No Hit
TGGTATAACAGATTGGTAGAGCATGACATTAAATTGTTGTGTCGGTGCCC11170.2645170609952188No Hit
GTACCTGACCTTTGAAACAGTTTAACTGGAGATAAGGTTGGGGAGGTGGC11160.2642802507347038No Hit
CGTACGCACCATGGAAGTCTTTCATAGAGAGATATGGTATAACAGATTGG10710.25362378901153027No Hit
GTTCTGAATTGACTGTTAATTGATGGCCTTTCATAGACGACACGTGAATG10310.24415137859093156No Hit
ACATAGTACCTGACCTTTGAAACAGTTTAACTGGAGATAAGGTTGGGGAG10140.24012560416217713No Hit
GTATTGGGTATTTATGTACTTTTTGATGTGATGATGTTGATGGTTTAGTT10000.2368102605149676No Hit
TAATTAGACAGTCGGATTCCCTAAACCGTGCCAGTTCTGAATTGACTGTT9560.22639060905230904No Hit
GTCCATGGAGAGGGCACCGACACAACAATTTAATGTCATGCTCTACCAAT9480.2244961269681893No Hit
AATATACATCGCACCGCGAAGCACAATGCATATTGTAAAGCCCGAGCCAC9430.22331207566561442No Hit
GCCTAGAGACATTAAAATGAATCCTGGGAAGAGTTATCTTTTCTATTTAA9430.22331207566561442No Hit
GTCGTACCCATAACCGCAGCAGGTCCTCAAGGTGAACAGCCTCTAGTTGA9190.2176286294132552No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACATTATCA8490.20105191117720747No Hit
GTGGTGGCTCGGGCTTTACAATATGCATTGTGCTTCGCGGTGCGATGTAT8260.19560527518536325No Hit
GTACAGGCTATTGAGAATTTGCGTATCCCTTTCATAGATTTTCAAGGTCC8120.19228993153815369No Hit
CACCATGGAAGTCTTTCATAGAGAGATATGGTATAACAGATTGGTAGAGC8000.18944820841197407No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACATCCACC7710.18258071085704No Hit
CCCCTTGTCCGTACCAGTTCTAAGTCGATTGTTAAACATAAGTTGAATAT7600.1799757979913754No Hit
ATTTAACATAGTACCTGACCTTTGAAACAGTTTAACTGGAGATAAGGTTG7510.17784450564674065No Hit
GTCTAGCAAAGAGCGAAAGGGAATATGGTTCCAATTCCATAACCTATTGA7450.17642364408365085No Hit
ATCCATTACAGACTTAATATACATCGCACCGCGAAGCACAATGCATATTG7420.17571321330210596No Hit
GTATAACAGCTGTAATTTCTTAGATCATATTCCCAGTACTTGGATAACTG7360.17429235173901614No Hit
TTCATAGAGAGATATGGTATAACAGATTGGTAGAGCATGACATTAAATTG7320.17334511069695627No Hit
ACATTATTCTATCAACTAGAGGCTGTTCACCTTGAGGACCTGCTGCGGTT7280.1723978696548964No Hit
TTGTAAAGCCCGAGCCACCACAGTCTTCAGAGCCAATCCTTATCCCGAAG7030.16647761314202222No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACATTATCACTAACC6980.16529356183944738No Hit
CCATGGAAGTCTTTCATAGAGAGATATGGTATAACAGATTGGTAGAGCAT6830.16174140793172287No Hit
GTCCGTACCAGTTCTAAGTCGATTGTTAAACATAAGTTGAATATAAATTA6670.15795244376348339No Hit
GTTAATTGATGGCCTTTCATAGACGACACGTGAATGCCATCCATTACAGA6560.15534753089781875No Hit
ATCACATTGTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTTAA6540.1548739103767888No Hit
TCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAAACCGTGCCAG6540.1548739103767888No Hit
TCCTCAAGGTGAACAGCCTCTAGTTGATAGAATAATGTAGATAAGGGAAG6470.15321623855318403No Hit
CCTTGAGGACCTGCTGCGGTTATGGGTACGACTTAGTGTAAAATTTTATA6350.15037451542700442No Hit
GCGAAGCACAATGCATATTGTAAAGCCCGAGCCACCACAGTCTTCAGAGC6280.14871684360339965No Hit
GATTGGTAGAGCATGACATTAAATTGTTGTGTCGGTGCCCTCTCCATGGA6240.14776960256133978No Hit
GTATACGTTTGTCATTGGTCTCCACGCAAGTGGATTAAAGGCTCTTGACA6240.14776960256133978No Hit
GAGTATACGTTTGTCATTGGTCTCCACGCAAGTGGATTAAAGGCTCTTGA6130.14516468969567514No Hit
ATCACATTGTGTCAACATTATCACTAACCTTCACAATGCTATGTTTTAAT6100.14445425891413025No Hit
ATACTTATCCAATCCTTAGAGCCAATTCTTTTTCCGAAGTTACGAATCTA6010.1423229665694955No Hit
GCTCTGAAGACTGTGGTGGCTCGGGCTTTACAATATGCATTGTGCTTCGC5960.14113891526692068No Hit
GATTAAGAGTCTCTAGTCGATAGAACAATGTAGGTAAGGGAAGTCGGCAA5950.1409021050064057No Hit
CCATTACAGACTTAATATACATCGCACCGCGAAGCACAATGCATATTGTA5900.1397180537038309No Hit
CTCAATAGCCTGTACCGAATCCGCAGCAGGTCTCCAAGATTAAGAGTCTC5860.13877081266177102No Hit
GCATATTGTAAAGCCCGAGCCACCACAGTCTTCAGAGCCAATCCTTATCC5850.13853400240125605No Hit
GTACATGGGCAGAAATCACATTGTGTCAACATTATCACTAACCTTCACAA5810.13758676135919617No Hit
TGCCGGGAGTGGGTAATTTACGCGCCTGCTGCCTTCCTTAGATGTGGTAG5810.13758676135919617No Hit
ACCGAGAACCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTA5790.13711314083816623No Hit
ACATCGCACCGCGAAGCACAATGCATATTGTAAAGCCCGAGCCACCACAG5760.13640271005662133No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACATCCACCGAGAAC5600.13261374588838185No Hit
CGTGAATGCCATCCATTACAGACTTAATATACATCGCACCGCGAAGCACA5570.13190331510683695No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT5510.13048245354374716No Hit
ACCCATAACCGCAGCAGGTCCTCAAGGTGAACAGCCTCTAGTTGATAGAA5490.1300088330227172No Hit
GTACATGGGGATTTGACACTGAAAGGGAATACAGTTAATATTCTGTAAAT5480.12977202276220223No Hit
GCACAATGCATATTGTAAAGCCCGAGCCACCACAGTCTTCAGAGCCAATC5480.12977202276220223No Hit
TTCCATAACCTATTGAGTATACGTTTGTCATTGGTCTCCACGCAAGTGGA5480.12977202276220223No Hit
GTTTTAATTAGACAGTCGGATTCCCTAAACCGTGCCAGTTCTGAATTGAC5450.12906159198065734No Hit
GTACATAAATACCCAATACTTATCCAATCCTTAGAGCCAATTCTTTTTCC5410.12811435093859747No Hit
ACATTAAATTGTTGTGTCGGTGCCCTCTCCATGGACCTTGAAAATCTATG5400.1278775406780825No Hit
TCACTAACCTTCACAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTT5380.12740392015705257No Hit
GTACATGGGCAGAAATCACATTGTGTCAACATCCACCGAGAACCATCACA5380.12740392015705257No Hit
GTATATTAAGTCTGTAATGGATGGCATTCACGTGTCGTCTATGAAAGGCC5330.12621986885447772No Hit
GGGTACGACTTAGTGTAAAATTTTATAAAAGCTCCTCTAGATTTTCAAGG5250.124325386770358No Hit
AAATCACATTGTGTCAACATTATCACTAACCTTCACAATGCTATGTTTTA5240.12408857650984301No Hit
GTCTCTAGTCGATAGAACAATGTAGGTAAGGGAAGTCGGCAAATTAGATC5240.12408857650984301No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTCGATTGTTAAACATAAG5090.12053642260211851No Hit
CCTTAGAGCCAATTCTTTTTCCGAAGTTACGAATCTATTTTGCCGACTTC5030.1191155610390287No Hit
TATCTACATTATTCTATCAACTAGAGGCTGTTCACCTTGAGGACCTGCTG5000.1184051302574838No Hit
ACACTAAGTCGTACCCATAACCGCAGCAGGTCCTCAAGGTGAACAGCCTC4980.11793150973645386No Hit
GGTTGGGGAGGTGGCAGAGCAGCACTTTTTAAGTGAGGTCCGGCGCATTC4980.11793150973645386No Hit
CTCTCATAGAGAAGCCGTTGGGAGGCATCGGAAGAGTTTTCTTTTCTGTT4980.11793150973645386No Hit
GCCTTTCATAGACGACACGTGAATGCCATCCATTACAGACTTAATATACA4890.11580021739181914No Hit
GAACAATGTAGGTAAGGGAAGTCGGCAAATTAGATCCGTAACTTCGGGAT4870.11532659687078922No Hit
AAATCACATTGTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTT4870.11532659687078922No Hit
GAATAATGTAGATAAGGGAAGTCGGCAAAATAGATTCGTAACTTCGGAAA4830.11437935582872935No Hit
ATCGTACGCACCATGGAAGTCTTTCATAGAGAGATATGGTATAACAGATT4810.1139057353076994No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTCGA4790.11343211478666948No Hit
CCTCTATACTAACATCAAAGTTGATAGGGCAGACATTTGAAAGATCTGTC4680.11082720192100483No Hit
GACTTAATATACATCGCACCGCGAAGCACAATGCATATTGTAAAGCCCGA4670.11059039166048987No Hit
TTTTAAGTGAGGTCCGGCGCATTCATTTTAATCCTTGAAAATCTAGAGGA4670.11059039166048987No Hit
TCACAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCA4660.1103535813999749No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACATTATCAC4560.10798547879482523No Hit
GTTAATATTCTGTAAATTAGATGTAAAATAAGGCGGTAACGCGGCGAGCC4560.10798547879482523No Hit
CCTATTGAGTATACGTTTGTCATTGGTCTCCACGCAAGTGGATTAAAGGC4560.10798547879482523No Hit
CATTATCACTAACCTTCACAATGCTATGTTTTAATTAGACAGTCAGATTC4550.10774866853431025No Hit
GTATCAACGCAGAGTACATGGGGATTTGACACTGAAAGGGAATACAGTTA4540.10751185827379528No Hit
GTCGGATTCCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGATGGC4530.10727504801328032No Hit
CCCTTATCTACATTATTCTATCAACTAGAGGCTGTTCACCTTGAGGACCT4510.10680142749225037No Hit
TACTTATCCAATCCTTAGAGCCAATTCTTTTTCCGAAGTTACGAATCTAT4500.10656461723173541No Hit
GTCGGTGCCCTCTCCATGGACCTTGAAAATCTATGAAAGGGATACGCAAA4480.10609099671070549No Hit
GCACTAGGCCATACGATCAGCAAAGTTATCTAGAGTTCCACCATATAAAA4460.10561737618967554No Hit
TACCAGTAATGTACGCTCGTCTCAAAGGTTAAGCCATGCATGTCTAAGTA4460.10561737618967554No Hit
CGATAGAACAATGTAGGTAAGGGAAGTCGGCAAATTAGATCCGTAACTTC4430.10490694540813066No Hit
GTACCGAATCCGCAGCAGGTCTCCAAGATTAAGAGTCTCTAGTCGATAGA4410.1044333248871007No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACATCCACCG4390.10395970436607078No Hit
CTGTTATACCATATCTCTCTATGAAAGACTTCCATGGTGCGTACGATTGT4340.10277565306349593No Hit
CTCTTAATCTTGGAGACCTGCTGCGGATTCGGTACAGGCTATTGAGAATT4320.102302032542466No Hit
GAGATATGGTATAACAGATTGGTAGAGCATGACATTAAATTGTTGTGTCG4280.10135479150040613No Hit
CCCATAACCGCAGCAGGTCCTCAAGGTGAACAGCCTCTAGTTGATAGAAT4270.10111798123989117No Hit
AAGTCGTACCCATAACCGCAGCAGGTCCTCAAGGTGAACAGCCTCTAGTT4240.10040755045834626No Hit
ACATTGTTCTATCGACTAGAGACTCTTAATCTTGGAGACCTGCTGCGGAT4240.10040755045834626No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)