FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002150476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235360
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG31551.3404996600951733Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG24771.052430319510537Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT14360.6101291638341264Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14000.5948334466349422No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC8910.37856900067980964Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG8850.3760197144799456Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8750.3717709041468389No Hit
AGCCATAACAGTGCTCTACCCCCGATGGTGATACTTGAGGCACTACCTAAATAGTTTTCGGAGAGAACCAGCTAT8200.34840244731475184No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA7900.3356560163154317Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG7750.32928280081577155Clontech Universal Primer Mix Long (96% over 26bp)
GTTATGGCTAGGGGGTCATTGCGACTTACCAAACCATTGCAAACTCCGAATACCGATGAGTGCGAGCTTGGGAGA7240.30761386811692726No Hit
GTGTAGACCCGAAACCAAGTGATCTACCCATGGCCAGGATGAAGGTGCGGTAACACGCCCTGGAGGTCCGAACCC6920.2940176750509857No Hit
GTGAATAAGTGCACATGGTGGATGCCTTGGCGATTACAGGCGATGAAGGACGTAGTAGCTTGCGATAAGCTGCGG6570.27914683888511216No Hit
GTGCAGGAATATTAACCTGCTTCCCATCAGCTACGCATCTCTGCCTCGCCTTAGGGGCCGACTCACCCTGCTCCG6490.2757477906186268No Hit
TCATACGACGGTGCAGGAATATTAACCTGCTTCCCATCAGCTACGCATCTCTGCCTCGCCTTAGGGGCCGACTCA6450.2740482664853841No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC6420.2727736233854521Clontech Universal Primer Mix Long (96% over 26bp)
TTCCAGCACCGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACAG6420.2727736233854521No Hit
GTACTGGTTCACTATCGGTCGATTACGAGTATTTAGCCTTGGAGGATGGTCCCCCCATGTTCAGACAGGATTTCT6360.270224337185588No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC6150.2613018354860639Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT5950.25280421481985044Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG5670.2409075458871516Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTGAAACCGTGTGCATACAAACAGTCGGAGCCTCTTCGTGGGGTGACGGCGTACCTTTTGTATAATGGGTCAG5620.23878314072059825No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACAGTCGCAGCCACCATTTTATTGCAAC5450.2315601631543168No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG5420.23028552005438474Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTACTAGGGCGTACCTTATCCCGAAGTTACGGTACCAATTTGCCGAGTTCCTTCTCCCGAGTTCTCTCAAGCGCC5230.22221278042148196No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5170.21966349422161796No Hit
GTATGTGATAGTCGATTGAATAGTGGAAGTCTCTGGAAAGTGACGCCATAGTGGGTGATAGCCCCGTACACGAAA4970.2111658735554045No Hit
GTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTAT4970.2111658735554045No Hit
ATCACATACAGGCTCATCCCATTTCGCTCGCCACTACTTTGGGAATCTCGGTTGATTTCTTTTCCTGCAGCTACT4720.20054384772263764No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTA4700.19969408565601632No Hit
GGGTGAGTCGGCCCCTAAGGCGAGGCAGAGATGCGTAGCTGATGGGAAGCAGGTTAATATTCCTGCACCGTCGTA4680.19884432358939497No Hit
GGTCTACACCCAGCGACTGTCGCCCTGTTCGGACTCGATTTCTCTACGGCTCCCCTATTCGGTTAACCTCGCCAC4480.1903467029231815No Hit
ATTCTAAGGCGCTTGAGAGAACTCGGGAGAAGGAACTCGGCAAATTGGTACCGTAACTTCGGGATAAGGTACGCC4300.1826988443235894No Hit
CCGTAAGCCCAAGGTTTCCTGTTCAACGTTCATCGGAGCAGGGTGAGTCGGCCCCTAAGGCGAGGCAGAGATGCG4030.17122705642420122No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC3980.16910265125764787Clontech Universal Primer Mix Long (96% over 26bp)
GTGCGAGCTTGGGAGACAGACATCGGGTGCTAACGTCCGGTGTCAAGAGGGAAACAACCCAGACCGCCAGCTAAG3920.16655336505778381No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC3880.16485384092454114Clontech Universal Primer Mix Long (96% over 26bp)
CCTTAGAATACTCATCTCGCCCACCTGTGTCGGTTTGCGGTACGGTCTCGTATGACTGAAGCTTAGAGGCTTTTC3880.16485384092454114No Hit
ATACGAGACCGTACCGCAAACCGACACAGGTGGGCGAGATGAGTATTCTAAGGCGCTTGAGAGAACTCGGGAGAA3850.1635791978246091No Hit
GTGGATGCCTTGGCGATTACAGGCGATGAAGGACGTAGTAGCTTGCGATAAGCTGCGGGGAGCTAGCAAACGAGC3800.16145479265805576No Hit
GTCTCGGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT3780.16060503059143438No Hit
TTCCTGCACCGTCGTATGATGCGATGGGGGGACGGATCGCGGAAGGTTGTCTAGCTGTTGGAATAGCTAGTTTTT3700.157205982324949No Hit
CTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACAGTCGCAGCCACCATTTTATTGCAACCC3510.14913324269204623No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3410.1448844323589395No Hit
ATACATAGGTATGCAAAGCGAACGCGGCGAACTGAAACATCTAAGTAGCTGCAGGAAAAGAAATCAACCGAGATT3400.14445955132562882No Hit
TGGTAGGAGAGCGTTCTGTAAGCCTGCGAAGGTGTCTTGTAAAGGATGCTGGAGGTATCAGAAGTGCGAATGCTG3380.1436097892590075No Hit
TAGTACCACCGTTTGAATTTCGTGTACGGGGCTATCACCCACTATGGCGTCACTTTCCAGAGACTTCCACTATTC3370.1431849082256968No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGGACTTCAATCAAGAGCTTGCACCCCATCATTTAATCTT3350.14233514615907544No Hit
GTCGTATGATGCGATGGGGGGACGGATCGCGGAAGGTTGTCTAGCTGTTGGAATAGCTAGTTTTTGACTCATAGA3310.14063562202583277No Hit
GACTCACCCTGCTCCGATGAACGTTGAACAGGAAACCTTGGGCTTACGGCGTGCGGGCTTTTCACCCGCATTATC3180.135112168592794No Hit
AGCTCGCACTCATCGGTATTCGGAGTTTGCAATGGTTTGGTAAGTCGCAATGACCCCCTAGCCATAACAGTGCTC3150.13383752549286201No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC3120.13256288239292996Clontech Universal Primer Mix Long (96% over 26bp)
ACGTAGTAGCTTGCGATAAGCTGCGGGGAGCTAGCAAACGAGCTTTGATCCGCAGATTTCCGAATGGGGAAACCC3080.13086335825968728No Hit
TTCCTGCACCGTCGTATGATGCGATGGGGGGACGGATCGCGGAAGGTTGTCTGACTGTTGGAATAGTCAGTTTTT3010.12788919102651258No Hit
ACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGGTGTGACGCCTGCCCGGTGCTGGAAGATTAAATGATGG2970.12618966689326988No Hit
GTTCACTATCGGTCGATTACGAGTATTTAGCCTTGGAGGATGGTCCCCCCATGTTCAGACAGGATTTCTCGTGTC2950.12533990482664853No Hit
GGCTAAAACAGTCAGGATGTTGGCTTAGAAGCAGCCACCATTTAAAGAAAGCGTAATAGCTCACTGATCGAGTCG2940.12491502379333785No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG2940.12491502379333785Clontech Universal Primer Mix Long (96% over 25bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG2860.12151597552685248Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGGTCTTTCCGTCTAGCCGCGGGTAGATTGCATCATCACAAACATTTCAACTTCG2830.12024133242692046No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC2830.12024133242692046Clontech Universal Primer Mix Long (96% over 26bp)
CTGTAAGCCTGCGAAGGTGTCTTGTAAAGGATGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAGCGATA2820.11981645139360979No Hit
GGCTTACGGCGTGCGGGCTTTTCACCCGCATTATCGCTACTCATGTCAGCATTCGCACTTCTGATACCTCCAGCA2750.11684228416043507No Hit
GTCGTATGATGCGATGGGGGGACGGATCGCGGAAGGTTGTCTGACTGTTGGAATAGTCAGTTTTTGACTCATAGA2690.11429299796057103No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT2620.11131883072739632Clontech SMART CDS Primer II A (96% over 26bp)
GAGCAGGGTGAGTCGGCCCCTAAGGCGAGGCAGAGATGCGTAGCTGATGGGAAGCAGGTTAATATTCCTGCACCG2610.11089394969408566No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTATTGTTACGGC2530.10749490142760026No Hit
GCTTAGTACCACCGTTTGAATTTCGTGTACGGGGCTATCACCCACTATGGCGTCACTTTCCAGAGACTTCCACTA2530.10749490142760026No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC2510.10664513936097893Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGTCTTTCCGTCTAGCCGCGGGTAGATTGCATCATCACAAACATTTCAACTTCGCTGAGTCTCGGGA2510.10664513936097893No Hit
GTGGTACTAAGCATACGACAAGTAGGGCGGGACACGAGAAATCCTGTCTGAACATGGGGGGACCATCCTCCAAGG2440.10367097212780421No Hit
GTACATGGGAGATCCTGAAACCGTGTGCATACAAACAGTCGGAGCCTCTTCGTGGGGTGACGGCGTACCTTTTGT2440.10367097212780421No Hit
GTTTTAGCCTTCCCACTTCGTTTTCCACTTAGCCAATCTTTGGGACCTTAGCTGGCGGTCTGGGTTGTTTCCCTC2420.10282121006118287No Hit
CGTATAGGGTGTGACGCCTGCCCGGTGCTGGAAGATTAAATGATGGGGTGCAAGCTCTTGATTGAAGTCCCAGTA2400.10197144799456152No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG2400.10197144799456152Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGCGTACCTTATCCCGAAGTTACGGTACCAATTTGCCGAGTTCCTTCTCCCGAGTTCTCTCAAGCGCCTTAGAAT2400.10197144799456152No Hit
CCCTAGTAGTTTGACCACTTTACTGTGGAAGGATGAACGGGTTGCAATAAAATGGTGGCTGCGACTGTTTAATAA2360.10027192386131882No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)