FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002150478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences233395
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG39931.7108335654148548Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG31151.3346472717924547Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT18790.8050729450073908Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12890.5522826110242294No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC11230.48115855095439064Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA10770.4614494740675678Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG10650.45630797574926624Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG9610.41174832365731917Clontech Universal Primer Mix Long (96% over 26bp)
CTACAGAGGTGTAGCGCTCTGCCGGCCATCAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGACAGACCGTTA8920.382184708327085No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC8480.3633325478266458Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG7370.3157736883823561Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7360.31534523018916427No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT7240.3102037318708627Clontech Universal Primer Mix Long (96% over 26bp)
CCTCTGTAGCGTCCGGTGCGCTCCCGACGTCCCTTGAAAATCCGCTGGAAGGAATAAGATTCGCGCCAGGTCGTA7100.3042053171661775No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC7070.3029199425866021Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG6700.28706698943850556Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC5310.22751130058484542Clontech Universal Primer Mix Long (96% over 26bp)
CCCTTAACCTATTCTCAAACTTTAAATATGTAAGAAGCTCTTGTTACTTAGTTGAACGTGAGCATTTGAATGTAT5160.22108442768696843No Hit
GTTCTAAGTTGGTTGTTAATCGTGCGCCGGACGGCCGAAGCCTGCCAAGGGCGTCCACGCCAGGTGCTGGGACCC5050.21637138756185867No Hit
GCTTAGGAGTGTGTAACAACTCACCGGCCGAATGCATTGGCCCTGAAAATGGATGGCGCTTAAGCGTGTTACCCA5040.21594292936866682No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4960.21251526382313246No Hit
CTCCTAAGCGGATTCCGACTTCCATGGCCACCGTCCGGCTGTCTGGATGCATTGACACCTTTTGTGGTGTCTGAT4960.21251526382313246No Hit
GTGCTGGGACCCGCCGGCCGGTTGCCCGGCTAGTGAGTCCCTGCTCCCGGCGCTTCGACCCAAGGCCCAACGCAC4730.20266072537972107No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC4660.19966151802737847Clontech Universal Primer Mix Long (96% over 26bp)
CAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGAGTTAAACAGTATGTGAAATTGTTGAAAGGGAAG4510.1932346451295015No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGCGA4270.1829516484928983No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC4120.17652477559502133Clontech Universal Primer Mix Long (96% over 26bp)
CTCTACTTGTGCGCTATCGGTCTCGGGCCAATATTTAGCTTTAGAAGAAATTTACCTCCCATTTTGACCTGCATT4070.17438248462906233No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG3890.16667023715160992Clontech Universal Primer Mix Long (96% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT3790.16238565521969195Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3770.16152873883330834No Hit
GTGTAACAACTCACCGGCCGAATGCATTGGCCCTGAAAATGGATGGCGCTTAAGCGTGTTACCCATACCTCGCCG3560.15253111677628056No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC3550.15210265858308875Clontech Universal Primer Mix Long (96% over 26bp)
ATCCTGATGTCTTCGGATGGATTTGAGTAAGAGCATAGCTGTTGGGACCCGAAAGATGGTGAACTATGCGTGAAT3510.15038882581032156No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGCGAATCTTATTCCTTCCAGCGG3480.14910345123074617No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC3470.14867499303755435Clontech Universal Primer Mix Long (96% over 26bp)
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTTG3440.14738961845797896No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG3360.14396195291244457Clontech Universal Primer Mix Long (96% over 26bp)
CTTGTGCGCCGTCGGGCGAAAGGGAAGCCGGTTAATATTCCGGCACCTGGATGCGGTTTCTTCACGGCAACGTAA3320.1422481201396774No Hit
ATCCTAAGCCCTAGGGTAGTTCCGTAAAAAGTGCGCACTTGTGCGCCGTCGGGCGAAAGGGAAGCCGGTTAATAT3260.13967737098052657No Hit
ACCCTAGGGCTTAGGATCGACTCACCCATGTCCAACTGCTGTTCACATGGAACCTTTCCCCACTTCAGTCCTCAA3230.13839199640095118No Hit
CATCAGGATCGGTCGATCGTGCGGGCTTGCGCCTTCCGACCTCCGTTCACTTTCATTCCGCGCCGGGGTTTGACA3120.13367895627584137No Hit
GGGTAGAGTTCTCTTTTCTACTTAACGGTCTGTCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTGATGGCCG2990.128108999764348No Hit
TGCCAAGGGCGTCCACGCCAGGTGCTGGGACCCGCCGGCCGGTTGCCCGGCTAGTGAGTCCCTGCTCCCGGCGCT2870.1229675014460464No Hit
ACTCTACCCAGGGCCCCCGCCGTCGTCTCCGCTTTCAGTTACGTTGCCGTGAAGAAACCGCATCCAGGTGCCGGA2760.11825446132093662No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2750.1178260031277448No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG2730.11696908674136122Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT2730.11696908674136122No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG2710.11611217035497762Clontech Universal Primer Mix Long (96% over 26bp)
CCCCCAAGGCTTCGTCACGGACCTCCACGCCTGCCTACTCGTCAGGGCATCATGTCAACCCTGACGGCGAGGTAT2700.11568371216178581No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG2680.11482679577540221Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA2670.11439833758221042Clontech Universal Primer Mix Long (96% over 26bp)
GTCCGTACCAGTTCTAAGTTGGTTGTTAATCGTGCGCCGGACGGCCGAAGCCTGCCAAGGGCGTCCACGCCAGGT2610.11182758842305962No Hit
CCCTAGGGTAGTTCCGTAAAAAGTGCGCACTTGTGCGCCGTCGGGCGAAAGGGAAGCCGGTTAATATTCCGGCAC2590.11097067203667602No Hit
GTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGCGAATCTTATTCC2570.11011375565029244No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT2530.10839992287752521Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT2520.10797146468433341No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC2480.10625763191156623No Hit
CATCAGACACCACAAAAGGTGTCAATGCATCCAGACAGCCGGACGGTGGCCATGGAAGTCGGAATCCGCTTAGGA2450.10497225733199084No Hit
CATCCAGACAGCCGGACGGTGGCCATGGAAGTCGGAATCCGCTTAGGAGTGTGTAACAACTCACCGGCCGAATGC2420.10368688275241543No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG2390.10240150817284004Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTATCCTGAGGGAAACTTCGGCAGGAACCAGCTACCAGATGGTTCGATTAGTCTTTCGCCCCTATACGCAAA2380.10197304997964823No Hit
ACCCGGGGTCACCCCCAAGGCTTCGTCACGGACCTCCACGCCTGCCTACTCGTCAGGGCATCATGTCAACCCTGA2360.10111613359326463No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)