FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002150479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences233395
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG26791.1478394995608303Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG23361.0008783392960432Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT16230.6953876475502903Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9920.4250305276462649No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA8870.380042417361126Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG8800.3770432100087834Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC7960.34105272178067225Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG6850.2934938623363825Clontech Universal Primer Mix Long (96% over 26bp)
CTACAGAGGTGTAGCGCTCTGCCGGCCATCAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGACAGACCGTTA6720.28792390582488914No Hit
CCTCTGTAGCGTCCGGTGCGCTCCCGACGTCCCTTGAAAATCCGCTGGAAGGAATAAGATTCGCGCCAGGTCGTA6710.2874954476316974No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG6340.2716424944836007Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC5980.256217999528696Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC5820.2493626684376272Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT5740.2459350028920928Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG5380.23051050793718803Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5270.22579746781207824No Hit
CCCTTAACCTATTCTCAAACTTTAAATATGTAAGAAGCTCTTGTTACTTAGTTGAACGTGAGCATTTGAATGTAT4870.20865914008440628No Hit
GTGCTGGGACCCGCCGGCCGGTTGCCCGGCTAGTGAGTCCCTGCTCCCGGCGCTTCGACCCAAGGCCCAACGCAC4480.19194927054992608No Hit
GCTTAGGAGTGTGTAACAACTCACCGGCCGAATGCATTGGCCCTGAAAATGGATGGCGCTTAAGCGTGTTACCCA4260.1825231902997065No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC4010.17181173546991152Clontech Universal Primer Mix Long (96% over 26bp)
CTCCTAAGCGGATTCCGACTTCCATGGCCACCGTCCGGCTGTCTGGATGCATTGACACCTTTTGTGGTGTCTGAT3790.16238565521969195No Hit
GTTCTAAGTTGGTTGTTAATCGTGCGCCGGACGGCCGAAGCCTGCCAAGGGCGTCCACGCCAGGTGCTGGGACCC3760.16110028064011656No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC3720.15938644786734935Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3640.15595878232181495No Hit
CAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGAGTTAAACAGTATGTGAAATTGTTGAAAGGGAAG3610.15467340774223953No Hit
CTCTACTTGTGCGCTATCGGTCTCGGGCCAATATTTAGCTTTAGAAGAAATTTACCTCCCATTTTGACCTGCATT3500.14996036761712975No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC3450.14781807665117075Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG3130.13410741446903318Clontech Universal Primer Mix Long (96% over 26bp)
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTTG3060.13110820711669058No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGCGA3050.1306797489234988No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG2920.1251097924120054Clontech Universal Primer Mix Long (96% over 25bp)
CTTGTGCGCCGTCGGGCGAAAGGGAAGCCGGTTAATATTCCGGCACCTGGATGCGGTTTCTTCACGGCAACGTAA2900.1242528760256218No Hit
CCCTAGGGTAGTTCCGTAAAAAGTGCGCACTTGTGCGCCGTCGGGCGAAAGGGAAGCCGGTTAATATTCCGGCAC2840.12168212686647101No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC2790.119539835900512Clontech Universal Primer Mix Long (96% over 26bp)
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGCGAATCTTATTCCTTCCAGCGG2790.119539835900512No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT2720.11654062854816942No Hit
GTGTAACAACTCACCGGCCGAATGCATTGGCCCTGAAAATGGATGGCGCTTAAGCGTGTTACCCATACCTCGCCG2720.11654062854816942No Hit
ATCCTAAGCCCTAGGGTAGTTCCGTAAAAAGTGCGCACTTGTGCGCCGTCGGGCGAAAGGGAAGCCGGTTAATAT2670.11439833758221042No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT2670.11439833758221042Clontech SMART CDS Primer II A (96% over 26bp)
GGGTAGAGTTCTCTTTTCTACTTAACGGTCTGTCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTGATGGCCG2670.11439833758221042No Hit
ATCCTGATGTCTTCGGATGGATTTGAGTAAGAGCATAGCTGTTGGGACCCGAAAGATGGTGAACTATGCGTGAAT2610.11182758842305962No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC2500.10711454829794984Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCT2390.10240150817284004Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)