FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002150490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353800
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCCAACGCACCCAA18330.5180893159977389No Hit
ATATATACCCGTCCATTATGACCAATACAATGTGTATATGAGCCTTTATAATGAACTGTTGTTCTGGCACGGCTA17510.49491237987563597No Hit
GTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCTCCACCAGGTGCTGGAAGG14070.3976823063877897No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATT12940.3657433578292821No Hit
ATCCTAAGCCATAGGGAAACTCCGTTTTAAAGTGCGCTCTCGGGCGCCGCCTGGCGAAAGGGAAACCGGTTAATA12470.3524590163934426No Hit
CCTTTGTGCTGTCCGGTGCGCTCTCGACGACCCTTGAAAATCCGCCGGAAGGAATGATTTTCACGCGAGGTCGTA12270.3468061051441492No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTTG12040.34030525720746185No Hit
GATATATACCCGTCCATTATGACCAATACAATGTGTATATGAGCCTTTATAATGAACTGTTGTTCTGGCACGGCT10930.30893159977388357No Hit
ATATGAGAGAAATGGATTTAGTTGAGTATAAAAATATCGCAAGGATAGATATATACCCGTCCATTATGACCAATA10830.30610514414923684No Hit
GCACAAAGGTGTGCTGCTCTACCTGCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGATAGACAGTTA10720.30299604296212546No Hit
GTGTATATGAGCCTTTATAATGAACTGTTGTTCTGGCACGGCTACGACCTTAGAGGCGTTCAGGCATAATCCGGC10240.28942905596382135No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGA10000.2826455624646693No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT9780.27642736009044655No Hit
GTCTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTAATGGCAGGTAGAGCAGCACACCTTTGTGCTGTCC9720.27473148671565856No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTT9600.27133973996608257No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC9100.2572074618428491No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGAAAATCATTCCTTCCGGCGG9050.2557942340305257No Hit
GTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCCAACGCACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGT8830.249576031656303No Hit
GTCCGGTGCGCTCTCGACGACCCTTGAAAATCCGCCGGAAGGAATGATTTTCACGCGAGGTCGTACTCATAACCG8360.23629169022046353No Hit
GTGATAGACAGTTAAGAAGAAAAGAGAACTCTTCCCGGGGCCCCCGCCGACGTCTCCACCTTCAGTTGCGTTGCC8240.23289994347088752No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCTCC7740.21876766534765407No Hit
GCTTAGGATCGACTAACCCGTGTCCAACTGCTGTTCACACGGAACCTTTCCCCACTTCAGTCCTCAAAGTTCTCA7240.20463538722442057No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAG7180.20293951384963257No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCTCCACCAGG7030.1986998304126625No Hit
GTACGAGAGGAACCACAGGTACAAACCAATGGCACAATACTTGTCCGAGCGGACATTGGTATGAAGCTACGTTTG6960.19672131147540983No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC6890.19474279253815716No Hit
TGGCATACCTGTGCAGTACGAGAGGAACCACAGGTACAAACCAATGGCACAATACTTGTCCGAGCGGACATTGGT6830.19304691916336914No Hit
ATCCTACGCTTTGTGAATTTTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCCACGTCGCTA6770.19135104578858114No Hit
GTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCTCCACCAGGTGCTGGAAGGCGCCGGCCGTG6590.18626342566421708No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG6430.18174109666478236Clontech Universal Primer Mix Long (96% over 26bp)
CCTTAGAGGCGTTCAGGCATAATCCGGCAAACGTAGCTTCATACCAATGTCCGCTCGGACAAGTATTGTGCCATT6300.17806670435274166No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTACTACCAA6220.1758055398530243No Hit
TCGTGAGACAGGTTAGTTTTACCCTACTGGTGGCATTATATGCTGTTGATATGGCATACCTGTGCAGTACGAGAG6220.1758055398530243No Hit
GAGTACGACCTCGCGTGAAAATCATTCCTTCCGGCGGATTTTCAAGGGTCGTCGAGAGCGCACCGGACAGCACAA6180.17467495760316562No Hit
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCTCTAGTAACG6090.17213114754098363No Hit
GACCAATACAATGTGTATATGAGCCTTTATAATGAACTGTTGTTCTGGCACGGCTACGACCTTAGAGGCGTTCAG5990.1693046919163369No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGC5920.16732617297908423No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5810.16421707179197287No Hit
GCGTAGGATTGTTCACCCTTTCAAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTA5720.16167326172979085No Hit
GCACCGGACAGCACAAAGGTGTGCTGCTCTACCTGCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGA5610.1585641605426795No Hit
GCCTTGGGGGTGACCCCGGGTAGAACGGCCTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAAC5500.1554550593555681No Hit
TTCCAGCACCTGGTGGAGGACGCTCTTGGCAGGGTTCGCCCGTCCGGCGCACGCTTAACAACCAACTTAGAACTG5380.1520633126059921No Hit
ATACAATGTGTATATGAGCCTTTATAATGAACTGTTGTTCTGGCACGGCTACGACCTTAGAGGCGTTCAGGCATA5380.1520633126059921No Hit
GTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGAAAATCATTCC5370.1517806670435274No Hit
AAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT5360.15149802148106273No Hit
TACCTGTGCAGTACGAGAGGAACCACAGGTACAAACCAATGGCACAATACTTGTCCGAGCGGACATTGGTATGAA5310.1500847936687394No Hit
ATCCTGATGTCTTCGGATGGATTTGAGTAAGAGCATAGCTGTTGGGACCCGAAAGATGGTGAACTATGCCTGAAT5300.14980214810627474No Hit
GCTCTACCTGCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGATAGACAGTTAAGAAGAAAAGAGAAC5260.14867156585641605No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTA5250.1483889202939514No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG5130.14499717354437536Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA5120.14471452798191067No Hit
AGGTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAG5110.14443188241944602No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT5010.14160542679479932No Hit
GTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAAACCGTGCCAGTT5010.14160542679479932No Hit
GTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTACTACCAATACCTCGCCG4980.14075749010740532No Hit
CATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATGA4960.14019219898247598No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCG4960.14019219898247598No Hit
CTCTCGTACTGCACAGGTATGCCATATCAACAGCATATAATGCCACCAGTAGGGTAAAACTAACCTGTCTCACGA4930.13934426229508198No Hit
CCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGAAAATCATTCCTTCCGGCGGATTTTCAAGGGTCGT4890.13821368004522327No Hit
GCTAATGCCTGGCCTTACGATCCTTTTGGCTTTAAAGAGTTTTTAACAAGAGGTGTCAGAAAAGTTACCACAGGG4830.13651780667043525No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAG4820.1362351611079706No Hit
TGCCAAGAGCGTCCTCCACCAGGTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCAACCTGCTCCCAGGGCTTCT4820.1362351611079706No Hit
CAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAAAAGCACGTGAAATTGTTAAAAGGGAAG4770.13482193329564726No Hit
GTATTGGTAGTACGCTTGAGCGCCATCCATTTTCAGGGCTAGTTCATTCGGCAGGTGAGTTGTTACACACTCCTT4760.13453928773318258No Hit
GTATATGAGCCTTTATAATGAACTGTTGTTCTGGCACGGCTACGACCTTAGAGGCGTTCAGGCATAATCCGGCAA4720.13340870548332392No Hit
CCCCCAAGGCTTCGTCACGGGCCTCCACGCCTGCCTACTCGCTAGGGCATCGTTTCTACCCTAGCGGCGAGGTAT4600.13001695873374788No Hit
TCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCG4580.12945166760881854No Hit
CTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTAGATGAACTAACACCTTTTGTGGTGTCTGAT4570.1291690220463539No Hit
CCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGATGGCCTTTACAAATATCAAATACCTTGCGATACTCAA4530.12803843979649518No Hit
ACCTTAACCTATTCTCAAACTTTAAATATGTAAGAAGTCCTTGTTACTTAGTTGAACGTGGACATTTGAATGTAT4500.12719050310910118No Hit
GTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTACTACCAATACCTCGC4450.12577727529677785No Hit
CTACCAATACCTCGCCGCTAGGGTAGAAACGATGCCCTAGCGAGTAGGCAGGCGTGGAGGCCCGTGACGAAGCCT4450.12577727529677785No Hit
GATAGATATATACCCGTCCATTATGACCAATACAATGTGTATATGAGCCTTTATAATGAACTGTTGTTCTGGCAC4400.12436404748445451No Hit
GACAAGTATTGTGCCATTGGTTTGTACCTGTGGTTCCTCTCGTACTGCACAGGTATGCCATATCAACAGCATATA4390.12408140192198983No Hit
GGTTAGTCGATCCTAAGCCATAGGGAAACTCCGTTTTAAAGTGCGCTCTCGGGCGCCGCCTGGCGAAAGGGAAAC4380.12379875635952514No Hit
ATTATGACCAATACAATGTGTATATGAGCCTTTATAATGAACTGTTGTTCTGGCACGGCTACGACCTTAGAGGCG4330.1223855285472018No Hit
CCCTAGCTCCGGACAAACCGATTTCAGGGTGATAGACAGTTAAGAAGAAAAGAGAACTCTTCCCGGGGCCCCCGC4310.12182023742227246No Hit
ACCCCGGGTAGAACGGCCTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAACTTTGAGGACTGA4300.1215375918598078No Hit
ACCCGGGGTCACCCCCAAGGCTTCGTCACGGGCCTCCACGCCTGCCTACTCGCTAGGGCATCGTTTCTACCCTAG4190.11842849067269644No Hit
TCCTCCACCAGGTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCC4180.11814584511023177No Hit
ACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAA4100.1158846806105144No Hit
GAGCTAGGGTTTAATGGCAGGTAGAGCAGCACACCTTTGTGCTGTCCGGTGCGCTCTCGACGACCCTTGAAAATC4050.11447145279819106No Hit
CTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAACTTTGAGGACTGAAGTGGGGAAAGGTTCCG4040.1141888072357264No Hit
ATACTTGTCCGAGCGGACATTGGTATGAAGCTACGTTTGCCGGATTATGCCTGAACGCCTCTAAGGTCGTAGCCG4010.11334087054833239No Hit
GTACATGGGACAATATGAGAGAAATGGATTTAGTTGAGTATAAAAATATCGCAAGGATAGATATATACCCGTCCA3990.11277557942340306No Hit
GTGTGGACAGAAACCACACGTTGAGTATAAGGACAAAAGCTGGCTTGATCTTGATATTCAGTACGTATCGGGACG3960.11192764273600904No Hit
GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGGTGGCATTATATGCTGTTGATATGGCATACCTGT3930.11107970604861503No Hit
CCCTTATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGC3890.10994912379875636No Hit
GTACGACCTCGCGTGAAAATCATTCCTTCCGGCGGATTTTCAAGGGTCGTCGAGAGCGCACCGGACAGCACAAAG3870.10938383267382704No Hit
GCTCTAGTAACGGCGAGTGAAGCAGCAATAGCTCAAATTTGAAATCTGGCGTCTTCGACGTCCGAGTTGTAATTT3840.10853589598643301No Hit
TCCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGATGGCCTTTACAAATATCAAATACCTTGCGATACTCA3830.10825325042396834No Hit
GGATTATCCGCGGCAACGCAACTGAAGGTGGAGACGTCGGCGGGGGCCCCGGGAAGAGTTCTCTTTTCTTCTTAA3810.107687959299039No Hit
GTGCTGTCCGGTGCGCTCTCGACGACCCTTGAAAATCCGCCGGAAGGAATGATTTTCACGCGAGGTCGTACTCAT3760.10627473148671567No Hit
GCTACGACCTTAGAGGCGTTCAGGCATAATCCGGCAAACGTAGCTTCATACCAATGTCCGCTCGGACAAGTATTG3750.10599208592425098No Hit
GGTTGGCACTAGCCTCACGGCCGGCGCCTTCCAGCACCTGGTGGAGGACGCTCTTGGCAGGGTTCGCCCGTCCGG3700.10457885811192763No Hit
ATGTGTATATGAGCCTTTATAATGAACTGTTGTTCTGGCACGGCTACGACCTTAGAGGCGTTCAGGCATAATCCG3690.10429621254946297No Hit
CTTCTTAACTGTCTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTAATGGCAGGTAGAGCAGCACACCTT3680.1040135669869983No Hit
GTCCGGAGCTAGGGTTTAATGGCAGGTAGAGCAGCACACCTTTGTGCTGTCCGGTGCGCTCTCGACGACCCTTGA3670.10373092142453362No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTTGGGCC3650.1031656302996043No Hit
GGGTAGAAACGATGCCCTAGCGAGTAGGCAGGCGTGGAGGCCCGTGACGAAGCCTTGGGGGTGACCCCGGGTAGA3640.10288298473713964No Hit
GGTATGCCATATCAACAGCATATAATGCCACCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGC3610.10203504804974563No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTT3600.10175240248728094No Hit
CTTCTATACACTCCATGTCGAAGACATTGCCAGGTGGGGAGTTTGACTGGGGCGGTACATCTCCAAAACAATAAC3580.10118711136235162No Hit
GTATCAACGCAGAGTACATGGGACAATATGAGAGAAATGGATTTAGTTGAGTATAAAAATATCGCAAGGATAGAT3570.10090446579988693No Hit
GGATAGATATATACCCGTCCATTATGACCAATACAATGTGTATATGAGCCTTTATAATGAACTGTTGTTCTGGCA3550.10033917467495759No Hit
GTCTAGATGAACTAACACCTTTTGTGGTGTCTGATAAGCGTACATTCCGGCACCTTAACCTCGCGTTCGGTTCAT3550.10033917467495759No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)