FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002150491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353800
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCCAACGCACCCAA15660.4426229508196721No Hit
ATATATACCCGTCCATTATGACCAATACAATGTGTATATGAGCCTTTATAATGAACTGTTGTTCTGGCACGGCTA15610.44120972300734884No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATT12370.3496325607687959No Hit
GTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCTCCACCAGGTGCTGGAAGG11670.3298473713962691No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTTG10780.3046919163369135No Hit
CCTTTGTGCTGTCCGGTGCGCTCTCGACGACCCTTGAAAATCCGCCGGAAGGAATGATTTTCACGCGAGGTCGTA10270.29027699265121537No Hit
ATCCTAAGCCATAGGGAAACTCCGTTTTAAAGTGCGCTCTCGGGCGCCGCCTGGCGAAAGGGAAACCGGTTAATA9910.2801017524024873No Hit
ATATGAGAGAAATGGATTTAGTTGAGTATAAAAATATCGCAAGGATAGATATATACCCGTCCATTATGACCAATA9640.2724703222159412No Hit
GATATATACCCGTCCATTATGACCAATACAATGTGTATATGAGCCTTTATAATGAACTGTTGTTCTGGCACGGCT9340.2639909553420011No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT9300.2628603730921425No Hit
GTGTATATGAGCCTTTATAATGAACTGTTGTTCTGGCACGGCTACGACCTTAGAGGCGTTCAGGCATAATCCGGC8870.25070661390616167No Hit
GTCTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTAATGGCAGGTAGAGCAGCACACCTTTGTGCTGTCC8770.247880158281515No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTT8370.23657433578292822No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGA7800.22046353872244204No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC7720.2182023742227247No Hit
GTCCGGTGCGCTCTCGACGACCCTTGAAAATCCGCCGGAAGGAATGATTTTCACGCGAGGTCGTACTCATAACCG7690.21735443753533068No Hit
GCACAAAGGTGTGCTGCTCTACCTGCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGATAGACAGTTA7690.21735443753533068No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGAAAATCATTCCTTCCGGCGG7410.20944036178631997No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCTCC7080.20011305822498587No Hit
GTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCCAACGCACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGT6560.18541548897682306No Hit
TGGCATACCTGTGCAGTACGAGAGGAACCACAGGTACAAACCAATGGCACAATACTTGTCCGAGCGGACATTGGT6550.1851328434143584No Hit
ATCCTACGCTTTGTGAATTTTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCCACGTCGCTA6530.18456755228942906No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAG6450.1823063877897117No Hit
GTGATAGACAGTTAAGAAGAAAAGAGAACTCTTCCCGGGGCCCCCGCCGACGTCTCCACCTTCAGTTGCGTTGCC6380.18032786885245902No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCTCCACCAGG6320.178631995477671No Hit
GTACGAGAGGAACCACAGGTACAAACCAATGGCACAATACTTGTCCGAGCGGACATTGGTATGAAGCTACGTTTG5990.1693046919163369No Hit
TCGTGAGACAGGTTAGTTTTACCCTACTGGTGGCATTATATGCTGTTGATATGGCATACCTGTGCAGTACGAGAG5890.1664782362916902No Hit
GCGTAGGATTGTTCACCCTTTCAAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTA5620.15884680610514415No Hit
GACCAATACAATGTGTATATGAGCCTTTATAATGAACTGTTGTTCTGGCACGGCTACGACCTTAGAGGCGTTCAG5610.1585641605426795No Hit
GTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCTCCACCAGGTGCTGGAAGGCGCCGGCCGTG5550.15686828716789147No Hit
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCTCTAGTAACG5520.15602035048049745No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC5470.1546071226681741No Hit
CCTTAGAGGCGTTCAGGCATAATCCGGCAAACGTAGCTTCATACCAATGTCCGCTCGGACAAGTATTGTGCCATT5410.1529112492933861No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGC5160.14584511023176935No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA5070.14330130016958734No Hit
GCTTAGGATCGACTAACCCGTGTCCAACTGCTGTTCACACGGAACCTTTCCCCACTTCAGTCCTCAAAGTTCTCA5030.14217071791972868No Hit
AAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT4910.13877897117015262No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTACTACCAA4870.13764838892029396No Hit
TTCCAGCACCTGGTGGAGGACGCTCTTGGCAGGGTTCGCCCGTCCGGCGCACGCTTAACAACCAACTTAGAACTG4760.13453928773318258No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTA4710.13312605992085924No Hit
GAGTACGACCTCGCGTGAAAATCATTCCTTCCGGCGGATTTTCAAGGGTCGTCGAGAGCGCACCGGACAGCACAA4640.13114754098360656No Hit
CATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATGA4630.13086489542114188No Hit
ATACAATGTGTATATGAGCCTTTATAATGAACTGTTGTTCTGGCACGGCTACGACCTTAGAGGCGTTCAGGCATA4580.12945166760881854No Hit
TACCTGTGCAGTACGAGAGGAACCACAGGTACAAACCAATGGCACAATACTTGTCCGAGCGGACATTGGTATGAA4550.12860373092142455No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCG4510.12747314867156584No Hit
GTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTACTACCAATACCTCGCCG4420.12492933860938384No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT4330.1223855285472018No Hit
AGGTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAG4320.12210288298473715No Hit
CTCTCGTACTGCACAGGTATGCCATATCAACAGCATATAATGCCACCAGTAGGGTAAAACTAACCTGTCTCACGA4300.1215375918598078No Hit
GTATATGAGCCTTTATAATGAACTGTTGTTCTGGCACGGCTACGACCTTAGAGGCGTTCAGGCATAATCCGGCAA4300.1215375918598078No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG4290.12125494629734314Clontech Universal Primer Mix Long (96% over 26bp)
ACCTTAACCTATTCTCAAACTTTAAATATGTAAGAAGTCCTTGTTACTTAGTTGAACGTGGACATTTGAATGTAT4230.1195590729225551No Hit
GCACCGGACAGCACAAAGGTGTGCTGCTCTACCTGCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGA4180.11814584511023177No Hit
ATCCTGATGTCTTCGGATGGATTTGAGTAAGAGCATAGCTGTTGGGACCCGAAAGATGGTGAACTATGCCTGAAT4110.11616732617297909No Hit
TCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCG4090.11560203504804975No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG4080.11531938948558507Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGAAAATCATTCC4040.1141888072357264No Hit
CCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGATGGCCTTTACAAATATCAAATACCTTGCGATACTCAA4000.11305822498586772No Hit
CTACCAATACCTCGCCGCTAGGGTAGAAACGATGCCCTAGCGAGTAGGCAGGCGTGGAGGCCCGTGACGAAGCCT3980.11249293386093838No Hit
GTATCAACGCAGAGTACATGGGACAATATGAGAGAAATGGATTTAGTTGAGTATAAAAATATCGCAAGGATAGAT3970.11221028829847372No Hit
TGCCAAGAGCGTCCTCCACCAGGTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCAACCTGCTCCCAGGGCTTCT3960.11192764273600904No Hit
ATTATGACCAATACAATGTGTATATGAGCCTTTATAATGAACTGTTGTTCTGGCACGGCTACGACCTTAGAGGCG3940.11136235161107971No Hit
GATAGATATATACCCGTCCATTATGACCAATACAATGTGTATATGAGCCTTTATAATGAACTGTTGTTCTGGCAC3810.107687959299039No Hit
GGTTAGTCGATCCTAAGCCATAGGGAAACTCCGTTTTAAAGTGCGCTCTCGGGCGCCGCCTGGCGAAAGGGAAAC3790.10712266817410968No Hit
CAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAAAAGCACGTGAAATTGTTAAAAGGGAAG3770.10655737704918034No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAG3760.10627473148671567No Hit
GGTTGGCACTAGCCTCACGGCCGGCGCCTTCCAGCACCTGGTGGAGGACGCTCTTGGCAGGGTTCGCCCGTCCGG3730.10542679479932165No Hit
CTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTAGATGAACTAACACCTTTTGTGGTGTCTGAT3730.10542679479932165No Hit
GACAAGTATTGTGCCATTGGTTTGTACCTGTGGTTCCTCTCGTACTGCACAGGTATGCCATATCAACAGCATATA3730.10542679479932165No Hit
GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGGTGGCATTATATGCTGTTGATATGGCATACCTGT3720.10514414923685697No Hit
GCTAATGCCTGGCCTTACGATCCTTTTGGCTTTAAAGAGTTTTTAACAAGAGGTGTCAGAAAAGTTACCACAGGG3720.10514414923685697No Hit
GTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTACTACCAATACCTCGC3710.10486150367439231No Hit
CCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGAAAATCATTCCTTCCGGCGGATTTTCAAGGGTCGT3710.10486150367439231No Hit
GCTCTACCTGCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGATAGACAGTTAAGAAGAAAAGAGAAC3690.10429621254946297No Hit
TCCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGATGGCCTTTACAAATATCAAATACCTTGCGATACTCA3680.1040135669869983No Hit
TCCTCCACCAGGTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCC3540.10005652911249294No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)