FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002150504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences516463
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG63411.2277743032898774Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG57361.1106313521007314Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT29420.5696439047908562Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27110.5249165961550004No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC19020.3682742035731505Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA18720.3624654621918705Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG18120.35084797942931056Clontech Universal Primer Mix Long (96% over 26bp)
CTCTACTTGTGCGCTATCGGTCTCTGGCCGGTATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATT17120.33148550815837724No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG16820.3256767667770973Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC16340.3163827805670493Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15070.2917924420529641No Hit
ATCCTAAGCCATAGGGAAGTTCCGTTTCAAAGTGCGCGCTAAGCGCCGATTGGCGAAAGGGAAGCCGGTTAATAT14270.27630246503621747No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGA13700.2652658564117855No Hit
CAAGTAGAGTGATCGAAAGATGAAAAGTACTTTGGAAAGAGAGTTAAAAAGCACGTGAAATTGTTGAAAGGGAAG13260.2567463690525749No Hit
CCGTTACTAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGAACCGAACGCGAGGTTAAGGTGCCGG12710.24609700985356162No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT12550.24299901445021233Clontech Universal Primer Mix Long (96% over 26bp)
ATCCTGATGTCCTCGGATGGATTTGAGTAAGAGCATAGCTGTTGGGACCCGAAAGATGGTGAACTATGCCTGAAT12540.24280538973750296No Hit
GCACAAAGGTGTGCCGCTCTACCAGCTATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGATGAGCTGTTA12500.24203089088666563No Hit
GTTCTAAGTTGGTTGTTAAGCGCGCGCCGGACGGCCGAAGCCTGCCAAGGGCGTCCGCCACCGGGTGCTGGAAGG12280.23777114720706033No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG11710.22673453858262838Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGCTAGTACCGACCTGCTCCCGAGGAATCTGCCCAAGGCCCAACGCGCCCAACCCTTAGAGCCAATCCTTTTCCC11610.22479829145553507No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAATCATTCCTTCCGGCGG11420.22111942191405773No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG11290.2186023006488364Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGTTATAGCTTATGGTGATGCGGCGAGGCTTGGGCGAGGTCCGCGCTTCGGCAAGGATGATGGCGTAATGGTTG11120.21531068053277777No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC10520.2036931977702178Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCTAGTAACG10250.19846533052706583No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC10190.19730358225080982Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTGGAAGGTGCCGGCCGTGAGGCTAGTACCGACCTGCTCCCGAGGAATCTGCCCAAGGCCCAACGCGCCCAA9740.18859047017888989No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9510.18413710178657525No Hit
GCTTAGGATCGACTAACCCGTGTCCAACTGCTGTTCACACGGAACCTTTCCCCACTTCAGTCCTCAAAGTTCTCA9430.18258810408490056No Hit
GTGCACCATCGACCGATCCTGATGTCCTCGGATGGATTTGAGTAAGAGCATAGCTGTTGGGACCCGAAAGATGGT9340.18084548167051656No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC9260.1792964839688419Clontech Universal Primer Mix Long (96% over 26bp)
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC9150.17716661212903925No Hit
GGTTAAGGTTGTTTCAACCCCAAGGCCTCTAATCATTCGCTTTACCTCATAAAACTGAAAACGTTACTGCTATCC8990.17406861672568993No Hit
CTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTAGATGAACTAACACCTTTTGTGGTGTCTGAT8740.16922799890795662No Hit
CTAGTAACGGTGCATTCAAATGTCCACGTTCAACTAAGAAACAAGGACTTCTTACATATTTAAAGTTTGAGAATA8700.1684535000571193No Hit
ATTCAGGCATAGTTCACCATCTTTCGGGTCCCAACAGCTATGCTCTTACTCAAATCCATCCGAGGACATCAGGAT8630.16709812706815397No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTT8620.16690450235544463No Hit
CATCAGGATCGGTCGATGGTGCACCTTGCGGTTCCCACCTCCGTTCACTTTCATTACGCGTAGGGGTTTGACACC8520.1649682552283513No Hit
CCCTTTACGTAGCTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAATGGGAGGTAAATTTCTTCTAAA8380.16225750925042065No Hit
CTATAACACTCCCCCGAAGGTGAGCTACATTCCTAAGGTCTTATCCGGCGGGCCTAGACGATGATGGCCTCGCGA8370.1620638845377113No Hit
GTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTTAAGCGTATTACCCATACCTCGCCG8050.15586789373101267No Hit
GTTAAGAAGAAAAGAGAACTCTTCCCGGGGCCCCCGCCGACGTCTCCACCTTCAGTTGCGTTGCCGCGGATAATC7900.15296352304037267No Hit
CTTCTGGGTAGCGACCGATCTAAGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTATGTGACCGGCC7850.15199539947682603No Hit
GTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAATCATTCC7700.14909102878618605No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTTAAGCGTATTACCCA7690.1488974040734767No Hit
TTTCTACCCTGGCGGCGAGGTATGGGTAATACGCTTAAGCGCCATCCATTTTCAGGGCTAGTTCATTCGGCAGGT7660.14831652993534872No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG7630.1477356557972207Clontech Universal Primer Mix Long (96% over 25bp)
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGCGCGTTG7590.14696115694638337No Hit
ACCCATACCTCGCCGCCAGGGTAGAAACGATGCCCTGGCGAGTAGGCAGGCGTGGAGGCCCGTGACGAAGCCTTC7540.14599303338283673No Hit
ATGGTGCACCTTGCGGTTCCCACCTCCGTTCACTTTCATTACGCGTAGGGGTTTGACACCCGAACACTCGCATAG7480.14483128510658072No Hit
ACCCCGGATCACTCCGAAGGCTTCGTCACGGGCCTCCACGCCTGCCTACTCGCCAGGGCATCGTTTCTACCCTGG7410.1434759121176154No Hit
GTCTAGATGAACTAACACCTTTTGTGGTGTCTGATGAGCGTATATTCCGGCACCTTAACCTCGCGTTCGGTTCAT7390.14308866269219675No Hit
GTACCGACCTGCTCCCGAGGAATCTGCCCAAGGCCCAACGCGCCCAACCCTTAGAGCCAATCCTTTTCCCGAAGT7390.14308866269219675No Hit
CATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATGA7360.14250778855406873No Hit
GAGTACGACCTGGCGTGAAAATCATTCCTTCCGGCGGATTTTCAAGGGTCGTCGGGAGCGCACCGGACGGCACAA7260.1405715414269754No Hit
GGGTAGAAACGATGCCCTGGCGAGTAGGCAGGCGTGGAGGCCCGTGACGAAGCCTTCGGAGTGATCCGGGGTAGA7230.1399906672888474No Hit
CTATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGATGAGCTGTTAAGAAGAAAAGAGAACTCTTCCCGGG7180.13902254372530073No Hit
GTCTAACATCTATGCGAGTGTTCGGGTGTCAAACCCCTACGCGTAATGAAAGTGAACGGAGGTGGGAACCGCAAG7150.13844166958717274No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGCGC7010.1357309236092421No Hit
ATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGTAACGTTTTCAGTTTTATGAGGTAAAGCGAAT6980.13515004947111411No Hit
ATCCGGGGTAGAACGGCCTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAACTTTGAGGACTGA6980.13515004947111411No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGCGCGCCGGACGGCCGAAGCCTGCCAAGGGCGTCCGCC6950.1345691753329861No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT6920.1339883011948581Clontech SMART CDS Primer II A (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC6810.13185842935505543Clontech Universal Primer Mix Long (96% over 26bp)
GTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTTAAGCGTATTACCCATACCTCGC6720.13011580694067146No Hit
CCCTTATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGC6640.1285668092389968No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC6610.1279859351008688Clontech Universal Primer Mix Long (96% over 26bp)
GTCTAGGCCCGCCGGATAAGACCTTAGGAATGTAGCTCACCTTCGGGGGAGTGTTATAGCTTATGGTGATGCGGC6590.12759868567545013No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6570.12721143625003148No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGCGCGCCGGACGGCCGAAGCCTGCCAAGGGCGTCCGCCACCGGG6530.12643693739919415No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTTAATTAG6270.12140269486875148No Hit
CCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAATCATTCCTTCCGGCGGATTTTCAAGGGTCGT6250.12101544544333281No Hit
ACCTTAACCTATTCTCAAACTTTAAATATGTAAGAAGTCCTTGTTTCTTAGTTGAACGTGGACATTTGAATGCAC6230.12062819601791415No Hit
CATCAGACACCACAAAAGGTGTTAGTTCATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGA6210.12024094659249548No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT6100.11811107475269284No Hit
GCCCTAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCGCAAGCCCGAGTTGTAATTTGTAGA6000.11617482762559951No Hit
GTTCTGACGTGCAAATCGATCGTCAAATTTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTC5980.11578757820018085No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGCGCGCCGGACGGCCG5960.11540032877476218No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC5910.11443220521121553Clontech Universal Primer Mix Long (96% over 26bp)
CTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAATTTGACGATCGATTTGCACGTCAGAACCGCTGCG5870.1136577063603782No Hit
TGCCTGAATAGGGTGAAGCCAGAGGAAACTCTGGTGGAGGCTCGCAGCGGTTCTGACGTGCAAATCGATCGTCAA5870.1136577063603782No Hit
GTACATGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTTAATTAGACAGTCA5800.11230233337141285No Hit
GGTTAGTCGATCCTAAGCCATAGGGAAGTTCCGTTTCAAAGTGCGCGCTAAGCGCCGATTGGCGAAAGGGAAGCC5800.11230233337141285No Hit
GGGTAGAACGGCCTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAACTTTGAGGACTGAAGTGG5680.10997883681890087No Hit
CCCTAGCTCCGGACAAACCGATTTCAGGGTGATGAGCTGTTAAGAAGAAAAGAGAACTCTTCCCGGGGCCCCCGC5660.10959158739348221No Hit
TGCCAAGGGCGTCCGCCACCGGGTGCTGGAAGGTGCCGGCCGTGAGGCTAGTACCGACCTGCTCCCGAGGAATCT5630.1090107132553542No Hit
CTGCTATCCTGAGGGAAACTTCGGCAGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAA5580.10804258969180755No Hit
CTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAACTTTGAGGACTGAAGTGGGGAAAGGTTCCG5560.10765534026638889No Hit
GGCCTAGACGATGATGGCCTCGCGACAGAGAATAAACCGGTCAGAAGACCGGCGGAACACTGCCACGAAGTCTGG5490.10629996727742354No Hit
GGGTAATACGCTTAAGCGCCATCCATTTTCAGGGCTAGTTCATTCGGCAGGTGAGTTGTTACACACTCCTTAGCG5420.10494459428845822No Hit
CTTTCTGACCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTT5390.10436372015033024No Hit
ACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAATC5380.10417009543762089No Hit
GTCATAGAGGGTGAGAATCCCGTATGTGACCGGCCCGCACCCTTTACGTAGCTCCTTCGACGAGTCGAGTTGTTT5370.10397647072491158No Hit
GTATTACCCATACCTCGCCGCCAGGGTAGAAACGATGCCCTGGCGAGTAGGCAGGCGTGGAGGCCCGTGACGAAG5360.10378284601220222No Hit
CGTCAGAACCGCTGCGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTATTCAGGCATAGTTCACCATCTTTCG5300.10262109773594623No Hit
GCACTAGAGGCCGTTCTACCCCGGATCACTCCGAAGGCTTCGTCACGGGCCTCCACGCCTGCCTACTCGCCAGGG5250.10165297417239957No Hit
GTTTGGGAATGCAGCTCTAAATGGGAGGTAAATTTCTTCTAAAGCTAAATACCGGCCAGAGACCGATAGCGCACA5230.10126572474698091No Hit
CTACCAGCTATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGATGAGCTGTTAAGAAGAAAAGAGAACTCT5220.10107210003427157No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTTA5200.1006848506088529No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)