FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002150505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences516463
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG48540.9398543554911Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG43500.8422675002855965Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT22610.4377854754358008Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC15910.308056917920548Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA15260.2954713115944414Clontech Universal Primer Mix Long (96% over 26bp)
CTCTACTTGTGCGCTATCGGTCTCTGGCCGGTATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATT15040.2912115679148361No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG14190.2747534673345428Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG12290.23796477191976967Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11950.23138153168765235No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC11660.2257664150190817Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT9900.19168846558223918Clontech Universal Primer Mix Long (96% over 26bp)
ATCCTAAGCCATAGGGAAGTTCCGTTTCAAAGTGCGCGCTAAGCGCCGATTGGCGAAAGGGAAGCCGGTTAATAT9770.18917134431701788No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG9740.18859047017888989Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC8590.16632362821731664Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAAGTAGAGTGATCGAAAGATGAAAAGTACTTTGGAAAGAGAGTTAAAAAGCACGTGAAATTGTTGAAAGGGAAG8180.158385014996234No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG8160.15799776557081532Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCCTGATGTCCTCGGATGGATTTGAGTAAGAGCATAGCTGTTGGGACCCGAAAGATGGTGAACTATGCCTGAAT7680.14870377936076737No Hit
CCGTTACTAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGAACCGAACGCGAGGTTAAGGTGCCGG7430.14386316154303408No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC7300.14134604027781272Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC7280.14095879085239407Clontech Universal Primer Mix Long (96% over 26bp)
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGCGCGTTG7220.13979704257613806No Hit
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCTAGTAACG7040.1363117977473701No Hit
CCTTTGTGCCGTCCGGTGCGCTCCCGACGACCCTTGAAAATCCGCCGGAAGGAATGATTTTCACGCCAGGTCGTA7040.1363117977473701No Hit
GTGTTATAGCTTATGGTGATGCGGCGAGGCTTGGGCGAGGTCCGCGCTTCGGCAAGGATGATGGCGTAATGGTTG6950.1345691753329861No Hit
GGGTAGAAACGATGCCCTGGCGAGTAGGCAGGCGTGGAGGCCCGTGACGAAGCCTTCGGAGTGATCCGGGGTAGA6710.12992218222796212No Hit
GCACAAAGGTGTGCCGCTCTACCAGCTATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGATGAGCTGTTA6550.1268241868246128No Hit
CTAGTAACGGTGCATTCAAATGTCCACGTTCAACTAAGAAACAAGGACTTCTTACATATTTAAAGTTTGAGAATA6360.12314531728313549No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6300.1219835690068795No Hit
GGCTAGTACCGACCTGCTCCCGAGGAATCTGCCCAAGGCCCAACGCGCCCAACCCTTAGAGCCAATCCTTTTCCC6120.1184983241781115No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG6080.11772382532727417Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTT6030.11675570176372752No Hit
ATTCAGGCATAGTTCACCATCTTTCGGGTCCCAACAGCTATGCTCTTACTCAAATCCATCCGAGGACATCAGGAT6020.11656207705101818No Hit
CTTCTGGGTAGCGACCGATCTAAGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTATGTGACCGGCC5990.11598120291289019No Hit
GGTTAAGGTTGTTTCAACCCCAAGGCCTCTAATCATTCGCTTTACCTCATAAAACTGAAAACGTTACTGCTATCC5970.11559395348747152No Hit
GTTCTAAGTTGGTTGTTAAGCGCGCGCCGGACGGCCGAAGCCTGCCAAGGGCGTCCGCCACCGGGTGCTGGAAGG5940.11501307934934352No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGA5890.11404495578579686No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAATCATTCCTTCCGGCGG5790.11210870865870352No Hit
CTATAACACTCCCCCGAAGGTGAGCTACATTCCTAAGGTCTTATCCGGCGGGCCTAGACGATGATGGCCTCGCGA5750.1113342098078662No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC5620.10881708854264488Clontech Universal Primer Mix Long (96% over 26bp)
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT5600.10842983911722621No Hit
CATCAGGATCGGTCGATGGTGCACCTTGCGGTTCCCACCTCCGTTCACTTTCATTACGCGTAGGGGTTTGACACC5490.10629996727742354No Hit
CTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTAGATGAACTAACACCTTTTGTGGTGTCTGAT5460.10571909313929556No Hit
CCCTTTACGTAGCTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAATGGGAGGTAAATTTCTTCTAAA5270.10204022359781824No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT5250.10165297417239957Clontech SMART CDS Primer II A (96% over 26bp)
CTTCTTAACAGCTCATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTAATAGCTGGTAGAGCGGCACACCTT5250.10165297417239957No Hit
GTGCACCATCGACCGATCCTGATGTCCTCGGATGGATTTGAGTAAGAGCATAGCTGTTGGGACCCGAAAGATGGT5220.10107210003427157No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)