FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002150513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences603478
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG53780.8911675322049851Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG46380.7685450008119601Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT22260.36886183092009983Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG18410.30506497337102595Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC17250.28584306304455176Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG15110.25038195261467694Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC14640.24259376480998482Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA14450.23944534846340712Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT12810.2122695442087367Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG10990.1821110297309927Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG10590.17548278479082915Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10020.16603753575109614Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC9600.15907787856392447Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC9350.15493522547632227Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTTAGGATCAGCCCCTGCCAGCGGGTCCCCTAAGCGTTTCGTCGCTTGCCACGTCCTTCATCGCCATCAGAAGC8930.14797556828915057No Hit
GTATCAACGCAGAGTACATGGGCAACAAGCTACTAAGGGCAATCGGTGGATGCCTAGGCACCAAGAGCCGATGAA8450.14002167436095433No Hit
GTACTGAGATGTTTCACTTCCCCACGTTCCCTCCAACCGCCCTATATATTCAGGCGGAGGTCGCCGGACATTACT8090.13405625391480716No Hit
ACTAAGGGCAATCGGTGGATGCCTAGGCACCAAGAGCCGATGAAGGACGTCGTAACCTGCGATAAGCCCCGGGGA6940.11500004971183705No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC6890.11417151909431662Clontech Universal Primer Mix Long (96% over 26bp)
GTACTAGTCCGCTATCGGTCACCAAGGAGTATTTAGGCTTAGCGGGTGGTCCCGCCGGATTCACAGCCAATTTCA6810.1128458701062839No Hit
CCCTCCAACCGCCCTATATATTCAGGCGGAGGTCGCCGGACATTACTCCGGTCTCTTTCGAGGTTTCCCTATTCG6800.11268016398277982No Hit
ACGCAGAGTACATGGGCAACAAGCTACTAAGGGCAATCGGTGGATGCCTAGGCACCAAGAGCCGATGAAGGACGT6690.11085739662423486No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC6380.10572050679560811Clontech Universal Primer Mix Long (96% over 26bp)
ATATATTCAGGCGGAGGTCGCCGGACATTACTCCGGTCTCTTTCGAGGTTTCCCTATTCGGAGATCCCCGGATCG6160.10207497207851818No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG6130.10157785370800591Clontech Universal Primer Mix Long (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)