FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002150528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334871
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG33991.0150177232426816Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG29850.8913880270313045Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT19180.572757867955123Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18440.5506598063134759No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA12070.36043730272254093Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG11550.3449089350824646Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC9950.2971293423437682Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC9530.2845871992498604Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9470.2827954645221593No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATT9180.2741354133382706No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG8290.24755801487737067Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG8210.24516903524043587Clontech Universal Primer Mix Long (96% over 26bp)
GTTCTAAGTTGCTTGTTAAGCGTATGCCGGATGCCCGAAAGCAGACGAGGTCATCCACATGCAGGTCCCGAGCAG7710.23023791250959325No Hit
GTATGGAGCTAGGGTTCAATGGCGGGTAGAGCTTGGCACTTTTGCTAAGTCCGGTGCGTTCTTGACAATCCTTGA7620.22755031041804158No Hit
GTCCCACCTGAGTCCCACAGCTTCCAACCAAGCCCCGACATACCCAAGCCTTAGAGCCAATCCTTTTCCCGAAGT7460.22277235114417193No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT7440.22217510623493822No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7270.21709852450645176No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT6720.20067428950252486No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT6500.1941045955009541Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC6340.18932663622708445Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGCTAGGGTTCAATGGCGGGTAGAGCTTGGCACTTTTGCTAAGTCCGGTGCGTTCTTGACAATCCTTGAAAATC6190.18484729940783168No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG6160.18395143204398112Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGCTTGGGTATGTCG5790.1729024012231576No Hit
GTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCCAACGCACCCAA5630.16812444194928794No Hit
TCCACATGCAGGTCCCGAGCAGGTCCATCCTGGCGAACCAGAGCAGTCCCACCTGAGTCCCACAGCTTCCAACCA5400.16125612549310034No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5230.15617954376461385No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC5170.1543878090369127Clontech Universal Primer Mix Long (96% over 26bp)
GTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCTCCACCAGGTGCTGGAAGG4910.14662362521687455No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGAAAATCATTCCTTCCGGCGG4860.1451305129437903No Hit
GTCCGTACCAGTTCTAAGTTGCTTGTTAAGCGTATGCCGGATGCCCGAAAGCAGACGAGGTCATCCACATGCAGG4840.14453326803455657No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAG4800.14333877821608917No Hit
GCACAAAGGTGTGCTGCTCTACCTGCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGATAGACAGTTA4750.14184566594300493No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGCTTGTTAAGCGTATGCCGGATGCCCGAAAGCAGACGAGGTCATCCACA4670.13945668630607008No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGA4650.1388594413968364No Hit
ATCCTAAGCCATAGGGAAACTCCGTTTTAAAGTGCGCTCTCGGGCGCCGCCTGGCGAAAGGGAAACCGGTTAATA4610.13766495157836897No Hit
ATCCTAAGAGATAGGGAAGCTCCGTTTTAAAGCGCGTAATTCTTTACGCCGCCTATCGAAAGGGAATCCAGTTAA4510.13467872703220046No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC4440.13258836984988248No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC4220.12601867584831175Clontech Universal Primer Mix Long (96% over 26bp)
CCTTTGTGCTGTCCGGTGCGCTCTCGACGACCCTTGAAAATCCGCCGGAAGGAATGATTTTCACGCGAGGTCGTA4220.12601867584831175No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA4120.1230324513021432No Hit
CCCTAGCTCCATACAATATGATTTCAGGGTGGTAGACGGTTAAGGAGAAAAGAAAACTCTTCCCGGGGTCCCTGC4100.1224352063929095No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTT4040.12064347166520839No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCTGTATTTAGCTTTAGATGGAATTTACCACCCATTTTGAGCTGCATTCC4010.11974760430135785No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC3950.11795586957365671Clontech Universal Primer Mix Long (96% over 26bp)
AACCAGAGCAGTCCCACCTGAGTCCCACAGCTTCCAACCAAGCCCCGACATACCCAAGCCTTAGAGCCAATCCTT3930.11735862466442301No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTA3910.11676137975518931No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG3870.1155668899367219Clontech Universal Primer Mix Long (96% over 25bp)
GTCTACCACCCTGAAATCATATTGTATGGAGCTAGGGTTCAATGGCGGGTAGAGCTTGGCACTTTTGCTAAGTCC3840.11467102257287135No Hit
GTCCCGAGCAGGTCCATCCTGGCGAACCAGAGCAGTCCCACCTGAGTCCCACAGCTTCCAACCAAGCCCCGACAT3730.11138617557208597No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGCTTGTTAAGCGTAT3710.11078893066285227No Hit
GTCTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTAATGGCAGGTAGAGCAGCACACCTTTGTGCTGTCC3710.11078893066285227No Hit
TCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCG3640.1086985734805343No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC3600.10750408366206687Clontech Universal Primer Mix Long (96% over 26bp)
GGTCCATCCTGGCGAACCAGAGCAGTCCCACCTGAGTCCCACAGCTTCCAACCAAGCCCCGACATACCCAAGCCT3490.10421923666128151No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGCTTGTTAAGCGTATGCCGGATGCCCGAAAGCAGACGAGGT3430.10242750193358038No Hit
GCTCCATACAATATGATTTCAGGGTGGTAGACGGTTAAGGAGAAAAGAAAACTCTTCCCGGGGTCCCTGCCAACG3430.10242750193358038No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG3420.10212887947896353Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT3370.10063576720587927Clontech SMART CDS Primer II A (96% over 26bp)
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCTCTAGTAACG3360.10033714475126243No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)