FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002150538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences599202
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46260.7720267956381988No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG26470.44175419975233726Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24330.4060400332442148No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG22270.37166097576443335Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17360.28971865915000283No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13180.2199592124191842No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT12120.20226901779366557Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG8650.14435866368937353Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC8440.14085400248997834Clontech Universal Primer Mix Long (96% over 26bp)
GGTCTACACCCAGCGACTGTCGCCCTGTTCGGACTCGATTTCTCTACGGCTCCCCTATTCGGTTAACCTCGCCAC8050.13434534597681583No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA7550.1260009145496844Clontech Universal Primer Mix Long (96% over 26bp)
GTGAATAAGTGCACATGGTGGATGCCTTGGCGATTACAGGCGATGAAGGACGTAGTAGCTTGCGATAAGCTGCGG7350.12266314197883184No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATT7180.11982603529360716No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG6980.1164882627227546Clontech Universal Primer Mix Long (96% over 26bp)
CTACTAGGGCGTACCTTATCCCGAAGTTACGGTACCAATTTGCCGAGTTCCTTCTCCCGAGTTCTCTCAAGCGCC6980.1164882627227546No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6640.11081404935230524No Hit
GTCGTATGATGCGATGGGGGGACGGATCGCGGAAGGTTGTCTAGCTGTTGGAATAGCTAGTTTTTGACTCATAGA6310.1053067246103985No Hit
CCTTTGTGCTGTCCGGTGCGCTCTCGACGACCCTTGAAAATCCGCCGGAAGGAATGATTTTCACGCGAGGTCGTA6210.10363783832497223No Hit
GTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCGCCACCGGGTGCTGGAAGG6120.10213584066808856No Hit
GTATGTGATAGTCGATTGAATAGTGGAAGTCTCTGGAAAGTGACGCCATAGTGGGTGATAGCCCCGTACACGAAA6100.1018020634110033No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6040.10080073163974752No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACAGTCGCAGCCACCATTTTATTGCAAC6030.10063384301120491No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)