FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002150562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448398
Sequences flagged as poor quality0
Sequence length75
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117342.616871618517478No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68731.5327900659681801No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52151.163029273101129No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGCCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA50871.1344832046530091TruSeq Adapter, Index 1 (96% over 28bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30520.6806453195598553No Hit
TTATACACATCTCCGAGCCCACGAGACCTGCCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA15430.34411393449569355TruSeq Adapter, Index 1 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCTGCCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA14100.31445278524881914TruSeq Adapter, Index 1 (96% over 28bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11170.24910905044179502No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT8070.17997404091900499No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7880.1757367338837372No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT7680.1712764106887185No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6160.1373779544065763No Hit
TATACACATCTCCGAGCCCACGAGACCTGCCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA5860.13068746961404823RNA PCR Primer, Index 1 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTTTTTTTTTTTTTTTTTTTTTTTTT5840.13024143729454635No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5800.1293493726555426Clontech SMART CDS Primer II A (100% over 21bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5600.1248890494605239No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTT5570.12422000098127112No Hit
ACACATCTCCGAGCCCACGAGACCTGCCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA5410.12065174242525613RNA PCR Primer, Index 1 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTTTTTTTTTTTTTTTTTTTTTTTTTT5190.11574538691073555No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5000.11150807987546776No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)