FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002150570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170691
Sequences flagged as poor quality0
Sequence length75
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47692.793937583118032No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28451.6667545447621728No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA24231.419524169405534RNA PCR Primer, Index 37 (96% over 28bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18941.1096074192546765No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13010.7621960150212959No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA6360.3726031249450762RNA PCR Primer, Index 37 (96% over 28bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5960.34916896614349907No Hit
TTATACACATCTCCGAGCCCACGAGACCGGAGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA4920.28824015325939856RNA PCR Primer, Index 28 (96% over 29bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4910.28765429928935915No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4770.27945234370880717No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3680.21559426097450948No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT2330.1365039750191867No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT2270.13298885119895015No Hit
ACACATCTCCGAGCCCACGAGACCGGAGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA2220.130059581348753RNA PCR Primer, Index 37 (96% over 28bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)