FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002150572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207069
Sequences flagged as poor quality0
Sequence length75
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53282.573055358358808No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31171.505295336337163No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA29541.42657761422521RNA PCR Primer, Index 37 (96% over 28bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21141.0209157334028753No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14890.7190839768386383No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA8190.3955203338017762RNA PCR Primer, Index 37 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCGGAGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA6540.3158367500688176RNA PCR Primer, Index 28 (96% over 29bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6230.30086589494323146No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5920.28589503981764536No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5410.2612655684820036No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4360.21055783337921175No Hit
ACACATCTCCGAGCCCACGAGACCGGAGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA2990.144396312340331RNA PCR Primer, Index 37 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT2750.1328059728882643No Hit
TATACACATCTCCGAGCCCACGAGACCGGAGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA2670.12894252640424206RNA PCR Primer, Index 37 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT2440.11783511776267813No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2300.11107408641563923No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGA2210.1067277091211142RNA PCR Primer, Index 37 (96% over 28bp)
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2200.10624477831061145No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)