FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002150578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28655
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA3691.2877333798638981RNA PCR Primer, Index 37 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1310.4571627988134706No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA1120.39085674402373055RNA PCR Primer, Index 37 (96% over 28bp)
GTTGATACCAGTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGG950.33153027394870005No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT620.2163671261559937No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT610.212877333798639No Hit
GTATCAACGCAGAGTACATGGGAAGCACTGGTATCAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGG590.2058977490839295No Hit
TATCAACGCAGAGTACATGGGAAGCACTGGTATCAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGA590.2058977490839295No Hit
TTATACACATCTCCGAGCCCACGAGACCGGAGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA470.16402024079567268RNA PCR Primer, Index 28 (96% over 29bp)
GTTGATACCAGTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGA470.16402024079567268No Hit
ACACATCTCCGAGCCCACGAGACCGGAGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA460.16053044843831793RNA PCR Primer, Index 37 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGGAGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACA360.12563252486477056RNA PCR Primer, Index 37 (96% over 28bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350.12214273250741581No Hit
GCGTTGATACCACCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGA340.11865294015006107No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.11167335543535159No Hit
GCGTTGATACCAGTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC310.10818356307799686No Hit
GTTGATACCAGTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCG290.10120397836328739No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGA290.10120397836328739RNA PCR Primer, Index 37 (96% over 28bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.10120397836328739No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)