FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002150582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225616
Sequences flagged as poor quality0
Sequence length75
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA65912.9213353662860793RNA PCR Primer, Index 37 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCGGAGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA20310.9002021133252961RNA PCR Primer, Index 28 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCGGAGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA18240.8084533011843132RNA PCR Primer, Index 37 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14330.6351499893624566No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7850.34793631657329266No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7570.33552584923055107No Hit
TATACACATCTCCGAGCCCACGAGACCGGAGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA6980.30937522161548825RNA PCR Primer, Index 37 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGGAGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGA5520.24466349904262108RNA PCR Primer, Index 37 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGGAGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA5340.23668534146514433RNA PCR Primer, Index 37 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGGAGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACA4630.2052159421317637RNA PCR Primer, Index 37 (96% over 28bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4310.19103255088291612No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGAAGAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA3230.14316360541805545RNA PCR Primer, Index 47 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGGAGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAC3180.140947450535423RNA PCR Primer, Index 37 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCGGAGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAGA2740.12144528756825756RNA PCR Primer, Index 37 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGGAGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAG2600.11524005389688674RNA PCR Primer, Index 37 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGGAGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAA2400.10637543436635699RNA PCR Primer, Index 37 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)