FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002150586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences915854
Sequences flagged as poor quality0
Sequence length100
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT868739.485463840306425No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT509185.559619764722325No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT359313.923223570569108No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272872.979405014336346No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124081.3548010927505914No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99241.0835788236989738No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89130.9731900499424582No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT53410.5831715535445605No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46740.5103433516695893No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40760.44504910171271844No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37040.4044312739803506Clontech SMART CDS Primer II A (100% over 21bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35550.38816230534561186No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32290.352567112225311No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA31090.33946458715035366No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCTCTCATCTCGTATGCCGT25680.28039403660408757No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGCTGTCT21600.23584545134923254No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT21210.23158713069987139No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCT20620.22514505587135067No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG19420.2120425307963933No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC18020.19675625154227638No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA17720.19348062027353705Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT16740.1827802247956552No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA16050.17524627287755473No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAG15920.173826832661101No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT15650.1708787645192356Clontech Universal Primer Mix Long (96% over 26bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGCC14600.15941405507864792No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG13280.1450012774961948Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATCCC12910.14096133226474963No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG12710.13877757808559008No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT11790.1287323088614561No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATA11760.12840474573458216No Hit
GTGTAGCCCTGGTCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTT11600.12665774239125452No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATAC11450.12501992675688484No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTGTCTCTTATA11450.12501992675688484No Hit
ATACACATCTCCGAGCCCACGAGACGCTCTCTCATCTCGTATGCCGTCTT11370.12414642508522102No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT11150.1217442954881455No Hit
GAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11090.12108916923439762Clontech SMARTer II A Oligonucleotide (95% over 21bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10640.11617572233128862Clontech Universal Primer Mix Long (95% over 21bp)
GTATCAACGCAGAGTACATGGGCATCCTCCAAGGCTAAATACTCCTGACT10620.11595734691337266No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCTCTTATAC10450.11410115586108704No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTATACACA10420.1137735927342131No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGACTTGACGT10330.11279090335359128No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10310.11257252793567533Clontech Universal Primer Mix Long (96% over 26bp)
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCC9510.10383751121903709No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACA9460.10329157267424721No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC9330.10187213245779349No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)