FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002150587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences915854
Sequences flagged as poor quality0
Sequence length100
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280643.064243864196695No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149641.633884876847183No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113531.2396080597999244No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72330.789754698893055No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT46190.5043380276769005No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39480.4310730749660972No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA33560.36643395126297423No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30720.3354246419189085No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22840.2493847272600218No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCT20230.22088673522198954No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGCTGTCT18690.20407182804246093No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG17810.19446330965415887No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT17510.19118767838541953No Hit
GAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16870.1841996650121089Clontech SMARTer II A Oligonucleotide (95% over 21bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA15420.1683674472132021No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA14810.16170699696676544Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC14490.15821299028011016No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT14090.15384548192179104No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13230.14445533895140492No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGCC12850.14030620601100174No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12780.1395418920482959No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12510.1365938239064305Clontech Universal Primer Mix Long (96% over 26bp)
GTAAATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA12400.13539275910789272No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12320.13451925743622892No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12000.13102525074957364Clontech SMART CDS Primer II A (100% over 21bp)
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG11360.12403723737626302No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG10970.11977891672690189Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA10970.11977891672690189No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATA10910.11912379047315402No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATCCC10890.11890541505523806No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTGTCTCTTATA10790.11781353796565827No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCTCTTATAC10680.11661247316712053No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT10180.11115308771922164No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10060.10984283521172587No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATAC9830.10733151790569238No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT9800.10700395477881847No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGACTTGACGT9720.10613045310715465No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACA9640.1052569514354908No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA9310.10165375703987754Clontech Universal Primer Mix Long (95% over 21bp)
GTATCAACGCAGAGTACATGGGCATCCTCCAAGGCTAAATACTCCTGACT9220.10067106765925572No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)