FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002150594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012466
Sequences flagged as poor quality0
Sequence length100
%GC31

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10883810.74979307947131No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT665686.57483806863638No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT376913.7226929101816757No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT348173.43883152619446No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177201.7501822283414947No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160071.5809913616852318No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148721.4688888318225009No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCTCTCATCTCGTATGCCGT122961.2144605349710509No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100630.9939099189503647No Hit
ATACACATCTCCGAGCCCACGAGACGCTCTCTCATCTCGTATGCCGTCTT84140.8310402522158769No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59080.5835257677788687No Hit
ACACATCTCCGAGCCCACGAGACGCTCTCTCATCTCGTATGCCGTCTTCT52120.5147827186295638Illumina PCR Primer Index 10 (95% over 22bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49400.48791761896201946No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG48710.4811025752963556Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA47650.4706330879259155No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47120.46539834424069554Clontech SMART CDS Primer II A (100% over 21bp)
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45830.45265717564836744No Hit
TTATACACATCTCCGAGCCCACGAGACGCTCTCTCATCTCGTATGCCGTC40530.40030973879616694No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT33580.33166545839563993Clontech Universal Primer Mix Long (96% over 26bp)
CATCTCCGAGCCCACGAGACGCTCTCTCATCTCGTATGCCGTCTTCTGCT29010.28652814020421424Illumina PCR Primer Index 10 (96% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG28060.2771451090703293Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT23320.23032872214968206Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA22740.22460013472057333No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA22660.22380998473035146Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATACACATCTCCGAGCCCACGAGACGCTCTCTCATCTCGTATGCCGTCT21790.21521710358668833No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT21120.20859959741858Clontech Universal Primer Mix Long (96% over 26bp)
GAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19180.18943846015569904Clontech SMARTer II A Oligonucleotide (95% over 21bp)
TCTCCGAGCCCACGAGACGCTCTCTCATCTCGTATGCCGTCTTCTGCTTG17640.1742280728439276Illumina PCR Primer Index 10 (96% over 27bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTGTCTCTTATA17110.16899332915870754No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC16840.16632657294170866Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATA16540.16336351047837658No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTATACACA16190.15990660427115577No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACA15910.15714107930537913No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT15650.15457309183715798Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA14790.14607897944227263Clontech Universal Primer Mix Long (95% over 21bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA14780.1459802106934949Clontech Universal Primer Mix Long (95% over 21bp)
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14520.14341222322527375No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC14410.14232576698871863Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTA14390.14212822949116316No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13100.12938706089883512No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT13010.1284981421598355No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC12980.12820183591350226No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT12820.12662153593305847Clontech SMART CDS Primer II A (100% over 23bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT12710.12553507969650338Clontech SMART CDS Primer II A (100% over 26bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12530.12375724221850412No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATAC12320.12168309849417167No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG12250.1209917172527275No Hit
GGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11980.1183249610357286Clontech Universal Primer Mix Long (95% over 21bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTT11610.11467051733095235No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCTCTTATAC11210.11071976737984288No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTT11150.11012715488717646No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC10960.10825054866039946No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10770.10637394243362246No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC10640.10508994869951188No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCCTGTCTCTTATACAC10600.10469487370440093No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACAC10440.10311457372395715No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACA10270.10143550499473564No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)