FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002150760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314087
Sequences flagged as poor quality0
Sequence length20-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81582.597369518636556No Hit
ATGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGC22790.7255951376529433No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC12910.41103261198330404No Hit
ATGGGCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCT12310.39192962459445946No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG10990.3499030523390016No Hit
GTCCAGTAAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCG8970.2855896614632252No Hit
TTACTGGACGGCTCGGGAGCAGATCGCCACTAGCCGGGCAACCGGCCGGC7790.24802045293183098No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA7560.24069764109944058No Hit
GTTCTAAGTTGGTTGTTAACCGTACGCCGGACGGCCGAAGCCTGCCAAAA7220.22987261491242872No Hit
GTCCACCGACAGCCGACGGGTTCGGGACTGGGACCCCCGTGCCCAGCCCT7220.22987261491242872No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA6840.21777405623282722No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC6560.20885932878469976No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT6470.20599388067637311No Hit
TTCCCAGTCCGTCCCCCGGCCGGCACGCGGCGACCCGCTCTCGCCGCGGG6440.20503873130693087No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAACCGTACGCCGGACGGCCGAAG6350.20217328319860423No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG6000.19102987388844492No Hit
ACCTATACCCGGCCGTCGGGGCAAGTGCCAGGCCCCGATGAGTAGGAGGG5890.18752765953382344No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT5680.18084161394772785No Hit
GTCTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTAATGGCTGGA5180.16492245779035744No Hit
ATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGGGAAAAGGA4980.15855479532740926No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGA4900.15600773034223003No Hit
TGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATG4890.15568934721908262No Hit
TATCTACATTGTTCTATCAACCAGAGGCTGTTCACCTTGGAGACCTGCTG4820.15346066535705077No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG4660.14836653538669223No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC4550.14486432103207073No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT4290.13658635983023812No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC4160.13244737922932182No Hit
GTTCTGAGTCGACTGTTCGACGCCCGGGGAAGACCGCCGAAGCGATCGTT4050.1289451648747003No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCCTGTCCGTACCA3990.12703486613581588No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT3990.12703486613581588No Hit
GCCTCTGGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATC3990.12703486613581588No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC3960.12607971676637367No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT3940.12544295052007884No Hit
ACCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTA3810.12130396991916254No Hit
TGCGCCGTATGGCGAAAGGGAAGCCGGTCAATATTCCGGCGCCTGGATTG3760.11971205430342548No Hit
GGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTC3700.11780175556454103No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC3580.11398115808677214No Hit
TTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTT3570.11366277496362473No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG3560.11334439184047733No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA3550.1130260087173299No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC3530.11238924247103509No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA3490.11111570997844546No Hit
GTTTTAATTAGACAGTCAGATTCCCCCTGTCCGTACCAGTTCTAAGTTGG3470.11047894373215064No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT3460.11016056060900324No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3460.11016056060900324Clontech Universal Primer Mix Long (96% over 26bp)
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT3390.1079318787469714No Hit
GACCGGGCGTGGATGGCACTCGGTCCTCCGGATTTTCAAGGGCCGCCGGG3330.10602158000808694No Hit
CCCCCTGTCCGTACCAGTTCTAAGTTGGTTGTTAACCGTACGCCGGACGG3290.10474804751549731No Hit
CCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGACGCCCGGGGAAGAC3220.10251936565346544No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)