FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002150761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314087
Sequences flagged as poor quality0
Sequence length20-100
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29000.9233110571274837No Hit
ATGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGC15710.5001798864645783No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC10370.3301632987038623No Hit
ATGGGCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCT9110.29004702518728886No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG8430.2683969728132651No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8150.2594822453651377No Hit
TTACTGGACGGCTCGGGAGCAGATCGCCACTAGCCGGGCAACCGGCCGGC7150.22764393305039687No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA6470.20599388067637311No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC6320.201218133829162No Hit
GTCCAGTAAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCG6120.19485047136621383No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG5200.16555922403665227No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT5130.1633305421746204No Hit
GTTCTAAGTTGGTTGTTAACCGTACGCCGGACGGCCGAAGCCTGCCAAAA5080.16173862655888335No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA5060.16110186031258855No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT4960.15791802908111446No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAACCGTACGCCGGACGGCCGAAG4900.15600773034223003No Hit
TTCCCAGTCCGTCCCCCGGCCGGCACGCGGCGACCCGCTCTCGCCGCGGG4610.14677461977095518No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGA4060.12926354799784773No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC4050.1289451648747003No Hit
ATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGGGAAAAGGA3880.12353265178119438No Hit
ACCTATACCCGGCCGTCGGGGCAAGTGCCAGGCCCCGATGAGTAGGAGGG3820.12162235304230994No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG3790.12066720367286772No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT3770.1200304374265729No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC3620.11525469057936177No Hit
ATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3540.11270762559418251No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA3400.10825026187011878No Hit
ATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3250.10347451502290766No Hit
TTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTT3230.10283774877661285No Hit
GTCTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTAATGGCTGGA3210.10220098253031804No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)