FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002150838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150838
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330223
Sequences flagged as poor quality0
Sequence length20-100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49961.5129170287956926No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT20960.634722596548393No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19120.5790026739506334No Hit
ATGGGGATCAGGACTTCTCAGATCATCTTCTCACCATGAGGCTCCCTGCT12650.3830744678595979No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12470.3776236058663388No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12270.37156709254049536No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11850.3588484145562241No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11020.33371388425397386No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9620.2913182909730697No Hit
ATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGG8970.2716346226640785No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG8680.26285267834160553No Hit
ATGGGGATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCT8110.24559161536295168No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT8040.24347183569890649No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8020.24286618436632218No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA7730.23408424004384915No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7410.22439381872249964No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC7050.21349209473598146No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA6890.20864688407530668No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6880.2083440584090145No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6800.20592145307867715No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG6790.20561862741238496No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA6700.2028931964157554No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC6670.2019847194168789No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6590.1995621140865415No Hit
ACCTGGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATG6450.1953225547584511No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6420.1944140777595746No Hit
AGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTC6310.1910829954303607No Hit
TTATTACTGCATGCAAAGTCTACAAGTTTCGTTGACGTTCGGCCAAGGGA6120.18532930777080944No Hit
TCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT6100.18472365643822508No Hit
GTCTACAAGTTTCGTTGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA6040.18290670244047205No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC5690.17230780412024602No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG5540.16776541912586343No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA5470.16564563946181823No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT5320.16110325446743565No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA5190.15716652080563742No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG5160.1562580438067609No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC4940.1495958791483331No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAA4890.14808175081687222No Hit
CTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTT4880.14777892515058005No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA4780.14475066848765836No Hit
GTCCCTGTCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA4500.13627154983147752No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA4410.13354611883484796No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG4270.12930655950675757No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA4240.12839808250788104No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA4230.12809525684158887No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGT4160.12597547717754365No Hit
ATGGGGGATCAGGACTTCTCAGATCATCTTCTCACCATGAGGCTCCCTGC4150.12567265151125148No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG4070.12325004618091412No Hit
TCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTG4020.12173591784945324No Hit
GCTAATGCTCTGGATACCTGGAACTGTGGCTGCACCATCTGTCTTCATCT4000.12113026651686891No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC3960.11991896385170021No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG3930.1190104868528237No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC3880.11749635852136284No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT3800.11507375319102546No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA3800.11507375319102546No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC3800.11507375319102546No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCT3800.11507375319102546No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG3780.11446810185844111No Hit
TTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAG3710.1123483221943959No Hit
ATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTA3710.1123483221943959No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG3690.11174267086181157No Hit
AGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGC3670.11113701952922721No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC3650.11053136819664287No Hit
GTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCC3630.10992571686405854No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGAACTGTG3620.10962289119776636No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC3620.10962289119776636No Hit
GTTTATTACTGCATGCAAAGTCTACAAGTTTCGTTGACGTTCGGCCAAGG3610.10932006553147418No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCA3560.10780593720001333No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC3550.10750311153372115No Hit
CTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGC3490.10568615753596812No Hit
AGTCTCCACTGTCCCTGTCCGTCACCCCTGGAGAGCCGGCCTCCATCTCC3430.10386920353821509No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT3410.10326355220563073No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC3400.10296072653933858No Hit
TCTCAGATCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTG3400.10296072653933858No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC3390.1026579008730464No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA3370.10205224954046206No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCT3370.10205224954046206No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG3340.10114377254158555No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT3330.10084094687529337No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)