FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002150839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330223
Sequences flagged as poor quality0
Sequence length20-100
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32520.9847890667821442No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT17420.5275223106809641No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14570.441216995787695No Hit
ATGGGGATCAGGACTTCTCAGATCATCTTCTCACCATGAGGCTCCCTGCT10480.3173612982741965No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9980.3022200149595879No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9430.28556460331351846No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9240.2798109156539672No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8360.253162257020256No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7640.23135880904721962No Hit
ATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGG7170.21712600273148752No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG7090.21470339740115013No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT6630.2007734167517102No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC6440.19501972909215895No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA6330.19168864676294503No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA5910.1789699687786738No Hit
ATGGGGATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCT5900.17866714311238163No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG5830.17654736344833644No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG5700.17261062978653818No Hit
ACCTGGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATG5610.16988519878990865No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC5540.16776541912586343No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA5510.16685694212698693No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT5450.1650399881292339No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT5360.16231455713260431No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG5240.15868064913709826No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA5140.15565239247417653No Hit
AGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTC4940.1495958791483331No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG4900.14838457648316442No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA4890.14808175081687222No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC4770.14444784282136616No Hit
GTCTACAAGTTTCGTTGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA4660.14111676049215227No Hit
TCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT4610.1396026321606914No Hit
TTATTACTGCATGCAAAGTCTACAAGTTTCGTTGACGTTCGGCCAAGGGA4470.135363072832601No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC4450.13475742150001666No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA4370.13233481616967926No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC4140.12536982584495932No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT4080.12355287184720627No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG4080.12355287184720627No Hit
CTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTT4060.12294722051462194No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAA4030.12203874351574542No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG3730.11295397352698025No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC3720.11265114786068808No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG3610.10932006553147418No Hit
GTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCC3600.109017239865182No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGT3560.10780593720001333No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA3470.10508050620338379No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC3410.10326355220563073No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG3380.10235507520675421No Hit
GTCCCTGTCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA3370.10205224954046206No Hit
TTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAG3350.1014465982078777No Hit
TCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTG3350.1014465982078777No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)