FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002150890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168928
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCGACCTGCTCCCGGGACTTCGACCCAAGGCCCAACGTGCCCAA11450.6778035612805455No Hit
TATCTACATTGTCCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGCGA6930.4102339458230726No Hit
ATCCTAAGGGGTAGGGAAGTTCCGTAAAAGTGCGCGCTCGCGCCGTCCCCCGAAAGGGAAGCCGGTTAACATTCC6220.36820420534192083No Hit
GTTCTAAGTTGGTTGTTAATCGCACGCCGGACGACCGAAGCCTGCCAAGGGCGTCCGCCGCCAGGTGCTGGAAGG6110.36169255540822126No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCGGTATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATT5620.33268611479446863No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGCACGTTG5550.3285423375639326No Hit
GGGTAGGGAAGTTCCGTAAAAGTGCGCGCTCGCGCCGTCCCCCGAAAGGGAAGCCGGTTAACATTCCGGCACCTG5040.29835196059859825No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG4850.2871045652585717Clontech Universal Primer Mix Long (96% over 26bp)
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGCGAAAGCTATTCCTTCCGGCGG4620.27348929721538173No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG4360.25809812464481907Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCCATACCTCGCCGCCAGGGTAGAAACGATGCCCTGGCGAGTAGGCAGGCGTGGGGGCCCGTGACGAAGCCCAG4190.2480346656563743No Hit
ATCCTGAAGTCTTCGGATGGATTTGAGTAAGAGCATAGCTGTTGGGACCCGAAAGATGGTGAACTATGCGTGAAT4000.2367872703163478No Hit
CCGTTACTAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGAACCGAACGCGAGGTTAAGGTGCCGG3970.2350113657889752No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTCCTATCAAC3950.23382742943739346No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3930.23264349308581173No Hit
GCCTCTAGTTGATAGGACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT3860.22849971585527565No Hit
GTCCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGCGAAAGCTATTCC3810.2255398749763213No Hit
CCTTAGGATCGACTAACCCGTGTCCAACTGCTGTTCACACGGAACCTTTCCCCACTTCAGTCCTCAAAGTTCTCA3760.22258003409736693No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGCTACCCA3620.21429247963629475No Hit
TCCCTACCCCTTAGGATCGACTAACCCGTGTCCAACTGCTGTTCACACGGAACCTTTCCCCACTTCAGTCCTCAA3390.20067721159310478No Hit
TGCCAAGGGCGTCCGCCGCCAGGTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCGACCTGCTCCCGGGACTTCG3350.19830933888994126No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTT3230.19120572078045084No Hit
GTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGCTACCCATACCTCGCCG3210.1900217844288691No Hit
CATCAGACACCACAAAAGGTGTTAGTTCATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAACCCGCTAAGGA3140.185878007198333No Hit
GCACCGGACCCCGCAAAGGTGCGGGGCTCTACCGGCCGTTAAACCCTAGCTCCGGACAAACCGTTTTCAGGGTGA3130.18528603902254215No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAATCGCACGCCGGACGACCGAAGCCTGCCAAGGGCGTCCGCC3020.17877438908884258No Hit
ACCGAAGCCTGCCAAGGGCGTCCGCCGCCAGGTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCGACCTGCTCCC2980.17640651638567909No Hit
CTCCTTAGCGGGTTCCGACTTCCATGGCCACCGTCCTGCTGTCTAGATGAACTAACACCTTTTGTGGTGTCTGAT2920.1728547073309339No Hit
GGTATGGGTAGCACGCTTGAGCGCCATCCATTTTCAGGGCTAGTTCATTCGGCAGGTGAGTTGTTACACACTCCT2910.172262739155143No Hit
CATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAACCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATGA2850.1687109301003978No Hit
CAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAAAAGTACGTGAAATTGTTGAAAGGGAAG2810.16634305739723432No Hit
ACCTTAACCTATTCTCAAACTTTAAATATGTAAGAAGTCCTTGTTGCTTAGTTGAACGTGGACATTCGAATGCAC2800.16575108922144347No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAATCGCACGCCGGACGACCGAAGCCTGCCAAGGGCGTCCGCCGCCAGG2790.16515912104565258No Hit
GTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGCTACCCATACCTCGC2760.16338321651827997No Hit
TTCCAGCACCTGGCGGCGGACGCCCTTGGCAGGCTTCGGTCGTCCGGCGTGCGATTAACAACCAACTTAGAACTG2750.16279124834248912No Hit
GGGTAGAAACGATGCCCTGGCGAGTAGGCAGGCGTGGGGGCCCGTGACGAAGCCCAGGGAGTGATCCCGGGTAGA2710.16042337563932563No Hit
CCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGCGAAAGCTATTCCTTCCGGCGGATTTTCAAGGGTCGT2710.16042337563932563No Hit
GTCCGGTGCGCCCCCGACGACCCTTGAAAATCCGCCGGAAGGAATAGCTTTCGCGCCAGGTCGTACTCATAACCG2690.1592394392877439No Hit
TGATAGGACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT2680.15864747111195301No Hit
GGGCTCTACCGGCCGTTAAACCCTAGCTCCGGACAAACCGTTTTCAGGGTGACGGGCCGTTAAGAAGAAAAGAGA2660.15746353476037128No Hit
GAGTACGACCTGGCGCGAAAGCTATTCCTTCCGGCGGATTTTCAAGGGTCGTCGGGGGCGCACCGGACCCCGCAA2640.15627959840878955No Hit
GGCTAGTGCCGACCTGCTCCCGGGACTTCGACCCAAGGCCCAACGTGCCCAACCCTTAGAGCCAATCCTTTTCCC2600.15391172570562606No Hit
GTGCCGACCTGCTCCCGGGACTTCGACCCAAGGCCCAACGTGCCCAACCCTTAGAGCCAATCCTTTTCCCGAAGT2540.15035991665088086No Hit
GCCCAGGGAGTGATCCCGGGTAGAACGGCCCCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAAC2470.14621613942034478No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGGACAATGTAGATAAGGGAAGTC2440.14444023489297214No Hit
CTCCCTGGGCTTCGTCACGGGCCCCCACGCCTGCCTACTCGCCAGGGCATCGTTTCTACCCTGGCGGCGAGGTAT2420.1432562985413904No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAATCGCAC2400.14207236218980868No Hit
CCCTAGCTCCGGACAAACCGTTTTCAGGGTGACGGGCCGTTAAGAAGAAAAGAGAACTCTTCCCAGGGCCCCCGC2400.14207236218980868No Hit
TTTCTACCCTGGCGGCGAGGTATGGGTAGCACGCTTGAGCGCCATCCATTTTCAGGGCTAGTTCATTCGGCAGGT2350.13911252131085433No Hit
CTAGTAACGGTGCATTCGAATGTCCACGTTCAACTAAGCAACAAGGACTTCTTACATATTTAAAGTTTGAGAATA2320.13733661678348172No Hit
ATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGTAACGTTTTCAGTTTTATGAGGTAAAGCGAAT2300.1361526804319No Hit
GGATAGCAGTAACGTTTTCAGTTTTATGAGGTAAAGCGAATGATTAGAGGCCTTGGGGTTGAAACAACCTTAACC2290.1355607122561091No Hit
ACCCGGGATCACTCCCTGGGCTTCGTCACGGGCCCCCACGCCTGCCTACTCGCCAGGGCATCGTTTCTACCCTGG2290.1355607122561091No Hit
GGTTAGTCGATCCTAAGGGGTAGGGAAGTTCCGTAAAAGTGCGCGCTCGCGCCGTCCCCCGAAAGGGAAGCCGGT2270.13437677590452737No Hit
CCTTTGCGGGGTCCGGTGCGCCCCCGACGACCCTTGAAAATCCGCCGGAAGGAATAGCTTTCGCGCCAGGTCGTA2250.13319283955294564No Hit
GTAGAGCCCCGCACCTTTGCGGGGTCCGGTGCGCCCCCGACGACCCTTGAAAATCCGCCGGAAGGAATAGCTTTC2220.13141693502557303No Hit
CTTCAGGATCGGTCGATGGTGCGCCTTGCGGCTCCCACCTCCGTTCACTTTCATTGCGCCTGAGGGTTTGACACC2210.13082496684978215No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT2200.1302329986739913Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAG2150.12727315779503695No Hit
GTTCTGACGTGCAAATCGATCGTCAAATTTGCGCATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTC2140.12668118961924607No Hit
GTCTAGATGAACTAACACCTTTTGTGGTGTCTGATGAGCGTGCATTCCGGCACCTTAACCTCGCGTTCGGTTCAT2140.12668118961924607No Hit
ATCCCGGGTAGAACGGCCCCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAACTTTGAGGACTGA2110.12490528509187346No Hit
GGGTAGAACGGCCCCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAACTTTGAGGACTGAAGTGG2060.12194544421291911No Hit
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCCAGTAACG2060.12194544421291911No Hit
GGCCCAACGTGCCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCT1970.11661773063080128No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAACTTTGAGGACTGAAGTGGGGAAAGGTTCCGTGTGA1940.11484182610342869No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1930.11424985792763782No Hit
GAGCTAGGGTTTAACGGCCGGTAGAGCCCCGCACCTTTGCGGGGTCCGGTGCGCCCCCGACGACCCTTGAAAATC1920.11365788975184693No Hit
GTTACACACTCCTTAGCGGGTTCCGACTTCCATGGCCACCGTCCTGCTGTCTAGATGAACTAACACCTTTTGTGG1890.11188198522447433No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTA1890.11188198522447433No Hit
GCGCTCAAGCGTGCTACCCATACCTCGCCGCCAGGGTAGAAACGATGCCCTGGCGAGTAGGCAGGCGTGGGGGCC1880.11129001704868347No Hit
GGGTTGGCACCCGGCCGCATACGGGATTCTCACCCTCTATGACGTCCTGTTCCAAGGAACTTAGGCCGGACCGCT1870.11069804887289258No Hit
AAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT1860.11010608069710172No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1850.10951411252131085No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1850.10951411252131085Clontech Universal Primer Mix Long (96% over 26bp)
GTGCCAACCCCGTGTGAGGCCCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAATGGGAGGTAAATTTC1840.10892214434551999No Hit
GTCTAACATCTGTGCGAGTCTTAGGGTGTCAAACCCTCAGGCGCAATGAAAGTGAACGGAGGTGGGAGCCGCAAG1820.10773820799393824No Hit
ACATTGTCCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGCGAAAGCT1810.10714623981814737No Hit
CCGCAAAGGTGCGGGGCTCTACCGGCCGTTAAACCCTAGCTCCGGACAAACCGTTTTCAGGGTGACGGGCCGTTA1760.10418639893919303No Hit
CCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGCTACC1760.10418639893919303No Hit
CTGCTATCCTGAGGGAAACTTCGGCAGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCCCCTATGCGCAAA1760.10418639893919303No Hit
TCCCAGGGCCCCCGCCGACGTCTCCGCCTTCAGTTGCGTTGCCGCGGAGAATCCGCATCCAGGTGCCGGAATGTT1750.10359443076340216No Hit
CTACTAGATGGTTCGATTAGTCTTTCGCCCCTATGCGCAAATTTGACGATCGATTTGCACGTCAGAACCGCTGCG1750.10359443076340216No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1740.1030024625876113Clontech Universal Primer Mix Long (96% over 26bp)
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAATCGCACGCCGGACGACCG1730.10241049441182043No Hit
CAGCAGGACGGTGGCCATGGAAGTCGGAACCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAACTAGCCC1720.10181852623602956No Hit
GTTATAGCCCGGGGTGCAATGCGGCCAGCCCGGACCGAGGACCGCGTTCGCTAGGATGCTGGCGTAATGGTCGTC1720.10181852623602956No Hit
CTTGAGCGCCATCCATTTTCAGGGCTAGTTCATTCGGCAGGTGAGTTGTTACACACTCCTTAGCGGGTTCCGACT1710.10122655806023867No Hit
CGACTAACCCGTGTCCAACTGCTGTTCACACGGAACCTTTCCCCACTTCAGTCCTCAAAGTTCTCATTTGAGTAT1700.1006345898844478No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)