FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002150891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168928
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCGACCTGCTCCCGGGACTTCGACCCAAGGCCCAACGTGCCCAA8450.5002131085432847No Hit
TATCTACATTGTCCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGCGA5730.3391977647281682No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCGGTATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATT5180.3066395150596704No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGCACGTTG4960.29361621519227127No Hit
ATCCTAAGGGGTAGGGAAGTTCCGTAAAAGTGCGCGCTCGCGCCGTCCCCCGAAAGGGAAGCCGGTTAACATTCC4810.28473669255540823No Hit
GTTCTAAGTTGGTTGTTAATCGCACGCCGGACGACCGAAGCCTGCCAAGGGCGTCCGCCGCCAGGTGCTGGAAGG4260.2521784428869104No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGCGAAAGCTATTCCTTCCGGCGG4090.2421149838984656No Hit
GGGTAGGGAAGTTCCGTAAAAGTGCGCGCTCGCGCCGTCCCCCGAAAGGGAAGCCGGTTAACATTCCGGCACCTG3780.22376397044894866No Hit
GCCTCTAGTTGATAGGACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT3530.20896476605417694No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG3430.20304508429626825Clontech Universal Primer Mix Long (96% over 26bp)
GTCCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGCGAAAGCTATTCC3150.1864699753741239No Hit
ACCCATACCTCGCCGCCAGGGTAGAAACGATGCCCTGGCGAGTAGGCAGGCGTGGGGGCCCGTGACGAAGCCCAG3060.18114226179200607No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTCCTATCAAC3040.17995832544042434No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG2960.17522258003409735Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCGTTACTAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGAACCGAACGCGAGGTTAAGGTGCCGG2940.17403864368251565No Hit
ATCCTGAAGTCTTCGGATGGATTTGAGTAAGAGCATAGCTGTTGGGACCCGAAAGATGGTGAACTATGCGTGAAT2940.17403864368251565No Hit
GTCCGGTGCGCCCCCGACGACCCTTGAAAATCCGCCGGAAGGAATAGCTTTCGCGCCAGGTCGTACTCATAACCG2830.16752699374881608No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGCTACCCA2770.16397518469407082No Hit
TGATAGGACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT2680.15864747111195301No Hit
ACCGAAGCCTGCCAAGGGCGTCCGCCGCCAGGTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCGACCTGCTCCC2650.1568715665845804No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTT2640.15627959840878955No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAATCGCACGCCGGACGACCGAAGCCTGCCAAGGGCGTCCGCC2630.15568763023299867No Hit
CCTTAGGATCGACTAACCCGTGTCCAACTGCTGTTCACACGGAACCTTTCCCCACTTCAGTCCTCAAAGTTCTCA2570.15213582117825347No Hit
TGCCAAGGGCGTCCGCCGCCAGGTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCGACCTGCTCCCGGGACTTCG2560.1515438530024626No Hit
TTCCAGCACCTGGCGGCGGACGCCCTTGGCAGGCTTCGGTCGTCCGGCGTGCGATTAACAACCAACTTAGAACTG2550.1509518848266717No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGGACAATGTAGATAAGGGAAGTC2530.14976794847508998No Hit
GGGTAGAAACGATGCCCTGGCGAGTAGGCAGGCGTGGGGGCCCGTGACGAAGCCCAGGGAGTGATCCCGGGTAGA2520.14917598029929913No Hit
CTCCTTAGCGGGTTCCGACTTCCATGGCCACCGTCCTGCTGTCTAGATGAACTAACACCTTTTGTGGTGTCTGAT2490.1474000757719265No Hit
TCCCTACCCCTTAGGATCGACTAACCCGTGTCCAACTGCTGTTCACACGGAACCTTTCCCCACTTCAGTCCTCAA2480.14680810759613563No Hit
GTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGCTACCCATACCTCGCCG2420.1432562985413904No Hit
GGTATGGGTAGCACGCTTGAGCGCCATCCATTTTCAGGGCTAGTTCATTCGGCAGGTGAGTTGTTACACACTCCT2380.14088842583822694No Hit
CATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAACCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATGA2380.14088842583822694No Hit
CATCAGACACCACAAAAGGTGTTAGTTCATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAACCCGCTAAGGA2350.13911252131085433No Hit
ACCTTAACCTATTCTCAAACTTTAAATATGTAAGAAGTCCTTGTTGCTTAGTTGAACGTGGACATTCGAATGCAC2290.1355607122561091No Hit
GGCTAGTGCCGACCTGCTCCCGGGACTTCGACCCAAGGCCCAACGTGCCCAACCCTTAGAGCCAATCCTTTTCCC2250.13319283955294564No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAATCGCACGCCGGACGACCGAAGCCTGCCAAGGGCGTCCGCCGCCAGG2180.12904906232240954No Hit
GAGCTAGGGTTTAACGGCCGGTAGAGCCCCGCACCTTTGCGGGGTCCGGTGCGCCCCCGACGACCCTTGAAAATC2170.12845709414661868No Hit
CTAGTAACGGTGCATTCGAATGTCCACGTTCAACTAAGCAACAAGGACTTCTTACATATTTAAAGTTTGAGAATA2130.1260892214434552No Hit
CTTCAGGATCGGTCGATGGTGCGCCTTGCGGCTCCCACCTCCGTTCACTTTCATTGCGCCTGAGGGTTTGACACC2130.1260892214434552No Hit
GGATAGCAGTAACGTTTTCAGTTTTATGAGGTAAAGCGAATGATTAGAGGCCTTGGGGTTGAAACAACCTTAACC2120.12549725326766434No Hit
GTGCCGACCTGCTCCCGGGACTTCGACCCAAGGCCCAACGTGCCCAACCCTTAGAGCCAATCCTTTTCCCGAAGT2110.12490528509187346No Hit
GTTCTGACGTGCAAATCGATCGTCAAATTTGCGCATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTC2070.12253741238870998No Hit
TTTCTACCCTGGCGGCGAGGTATGGGTAGCACGCTTGAGCGCCATCCATTTTCAGGGCTAGTTCATTCGGCAGGT2040.12076150786133738No Hit
CCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGCGAAAGCTATTCCTTCCGGCGGATTTTCAAGGGTCGT2010.11898560333396477No Hit
GTAGAGCCCCGCACCTTTGCGGGGTCCGGTGCGCCCCCGACGACCCTTGAAAATCCGCCGGAAGGAATAGCTTTC2000.1183936351581739No Hit
GTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGCTACCCATACCTCGC1940.11484182610342869No Hit
GCACCGGACCCCGCAAAGGTGCGGGGCTCTACCGGCCGTTAAACCCTAGCTCCGGACAAACCGTTTTCAGGGTGA1940.11484182610342869No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1930.11424985792763782No Hit
GTCTAGATGAACTAACACCTTTTGTGGTGTCTGATGAGCGTGCATTCCGGCACCTTAACCTCGCGTTCGGTTCAT1930.11424985792763782No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAG1920.11365788975184693No Hit
GGTTAGTCGATCCTAAGGGGTAGGGAAGTTCCGTAAAAGTGCGCGCTCGCGCCGTCCCCCGAAAGGGAAGCCGGT1890.11188198522447433No Hit
CCTTTGCGGGGTCCGGTGCGCCCCCGACGACCCTTGAAAATCCGCCGGAAGGAATAGCTTTCGCGCCAGGTCGTA1870.11069804887289258No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT1820.10773820799393824Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGTAACGTTTTCAGTTTTATGAGGTAAAGCGAAT1810.10714623981814737No Hit
CTCCCTGGGCTTCGTCACGGGCCCCCACGCCTGCCTACTCGCCAGGGCATCGTTTCTACCCTGGCGGCGAGGTAT1810.10714623981814737No Hit
CAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAAAAGTACGTGAAATTGTTGAAAGGGAAG1790.10596230346656564No Hit
GGGCTCTACCGGCCGTTAAACCCTAGCTCCGGACAAACCGTTTTCAGGGTGACGGGCCGTTAAGAAGAAAAGAGA1790.10596230346656564No Hit
CCCTAGCTCCGGACAAACCGTTTTCAGGGTGACGGGCCGTTAAGAAGAAAAGAGAACTCTTCCCAGGGCCCCCGC1750.10359443076340216No Hit
GGCCCAACGTGCCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCT1720.10181852623602956No Hit
TCTCCAAGGTGAACAGCCTCTAGTTGATAGGACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCG1710.10122655806023867No Hit
GTTACACACTCCTTAGCGGGTTCCGACTTCCATGGCCACCGTCCTGCTGTCTAGATGAACTAACACCTTTTGTGG1700.1006345898844478No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)