FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002150895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191689
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG13570.7079175122203152Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG10200.532111910438262Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT5900.30779022270448486Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC4710.245710499820021Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG4570.23840700301008405Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC4330.22588672276447788Clontech Universal Primer Mix Long (96% over 26bp)
GTCCTTAACAGTCCTTGAAAAACCGATGGAACGTATTACTCTCATGCCTGGTCGTACTCATAACCGCAGCAGGTC4200.2191049042981079No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG4180.21806154761097402Clontech Universal Primer Mix Long (96% over 26bp)
GTCCCAGCCAGACCCACTCCAGTTGCCCAGAGCAGTTCCAGCCGGGTCCAAACCCTTCCAGCCAAGGCCCGATGC3930.20501958902180095No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA3850.20084616227326554Clontech Universal Primer Mix Long (96% over 26bp)
GTTCTAAGTTGGTTGTTAATTGTACACCAGACGCCCGAAGGCAGCCTAAGCCTTTCAAGCTCAGTCCCAGCCAGA3490.1820657419048563No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT3330.17371888840778554No Hit
GTTAAGGACGCACCGGATGCAACAGAAGTGCTGCACTTTGCCCGTCATTGAACCTTATCTCCGGATAATCCGATT3300.17215385337708475No Hit
GTCTATAACCTTGAAATCGGATTATCCGGAGATAAGGTTCAATGACGGGCAAAGTGCAGCACTTCTGTTGCATCC3200.16693706994141552No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG2880.15024336294727397Clontech Universal Primer Mix Long (96% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT2790.14554825785517167Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG2750.14346154448090395Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTAAGGGCCCTGGGAAGAGTTATCTTTTCTCCTTGACCGTCTATAACCTTGAAATCGGATTATCCGGAGATAAG2700.14085315276306934No Hit
GTTCCAGCCGGGTCCAAACCCTTCCAGCCAAGGCCCGATGCACCCAACCCTTAGAGCCAATCCTTATCCCGAAGT2670.13928811773236857No Hit
CATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATGA2630.13720140435810088No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC2540.13250629926599858Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC2530.13198462092243166Clontech Universal Primer Mix Long (96% over 26bp)
GTATTACTCTCATGCCTGGTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACA2510.13094126423529778No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2470.12885455086103012No Hit
GTTATAGACGGTCAAGGAGAAAAGATAACTCTTCCCAGGGCCCTTACCAGCGTCACCAACTTCATTTACGTTACC2340.12207273239466011No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC2330.12155105405109318No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2310.12050769736395932Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGCATGA2260.1178993056461247No Hit
ACCCATACCTCACCGTCAGCGTTTATATGATGCGCTGACGAGTAGGCAGGCGTGGAGGTCAGTGAAGAAGCCTTG2200.11476923558472317No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGATAAGGATTGGCTCTAAGGGTTGGGTGCATCG2130.11111748717975471No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTA2080.10850909546192009No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA2050.10694406043121932No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAG2050.10694406043121932No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT2030.10590070374408547Clontech SMART CDS Primer II A (96% over 26bp)
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTG2010.10485734705695163No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)