FastQCFastQC Report
Wed 8 May 2024
EGAF00002150910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113602
Total Bases8.5 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50794.470871991690287No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28582.515800778155314No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23222.0439780989771306No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12051.0607207619584162No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5220.4594989524832309Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5020.4418936286332987No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4920.4330909667083326Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2990.2631995915564867No Hit
GTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGG2960.26055879297899687No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2320.20422175665921377Clontech SMART CDS Primer II A (100% over 21bp)
GTCCCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGG2280.2007006918892273No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTT2190.1927782961567578No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG2180.1918980299642612Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2150.18925723138677136Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2060.18133483565430186No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGA2040.17957430326930862No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2020.17781377088431544No Hit
GATATGAAGGGTCCACGGTGGATGCCCTGGCACTGGAGCCGATGAAGGAC1990.1751729723068256No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1810.1593281808418866Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGC1790.15756764845689336No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC1770.15580711607190012Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1750.15404658368690693Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1750.15404658368690693No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT1690.14876498653192727No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1670.14700445414693403No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC1630.1434833893769476Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGGTACGACCGTACCGCAAACCGACACTGGTGGGCGGGATGAGTATTCTC1610.14172285699195436No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA1590.13996232460696115Clontech SMART CDS Primer II A (96% over 26bp)
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGG1550.1364412598369747No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCA1540.1355609936444781No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACA1530.13468072745198148No Hit
AAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTT1500.13203992887449165No Hit
TTCCTGCACCGATACCAAGTGCGATGGGGGGACGGATCGCGAAATGTTGT1500.13203992887449165No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1470.1293991302970018No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTT1420.12499779933451875No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAG1420.12499779933451875No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTA1400.12323726694952553No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1340.11795566979454589Clontech Universal Primer Mix Long (96% over 26bp)
GTACTAGTTCACTATCGGTCGATCACGAGTATTTAGCCTTGGAGGATGGT1320.11619513740955265No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1250.11003327406207639Clontech Universal Primer Mix Long (96% over 26bp)
TCCCTATACGGTTAAGCTTGCCACTGAACGTAAGTCGCTGACCCATTATA1240.10915300786957975No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1240.10915300786957975No Hit
GTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGG1230.10827274167708315No Hit
GTCCACGGTGGATGCCCTGGCACTGGAGCCGATGAAGGACGCGATTACCT1190.10475167690709672No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)