FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002150910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113602
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9490.8353726166792839No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5330.4691818806006937No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG4540.39964085139346134Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4450.39171845566099184No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG4400.3873171246985088Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3120.2746430520589426No Hit
GTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGCCAGCATCCCAGCGCTGAAAGG2700.23767187197408496No Hit
GTCCCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT2220.19541909473424762No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTT2030.17869403707681203No Hit
GATATGAAGGGTCCACGGTGGATGCCCTGGCACTGGAGCCGATGAAGGACGCGATTACCTGCGAAAAGCCCTGAC1950.17165190753683912No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1930.16989137515184594Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT1920.1690111089593493Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTG1900.1672505765743561No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1550.1364412598369747Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG1530.13468072745198148Clontech Universal Primer Mix Long (96% over 26bp)
TGGTACGACCGTACCGCAAACCGACACTGGTGGGCGGGATGAGTATTCTCAGGCGCTTGAGAGAACCCAGGAGAA1510.13292019506698827No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGCCAGCA1480.13027939648949843No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT1450.1276385979120086Clontech SMART CDS Primer II A (96% over 26bp)
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACAGTCGCAGCCACCGATTCTGTGCCAC1450.1276385979120086No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT1450.1276385979120086Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG1440.12675833171951198Clontech Universal Primer Mix Long (96% over 25bp)
TTCCTGCACCGATACCAAGTGCGATGGGGGGACGGATCGCGAAATGTTGTCCGGGTGTTGGACGTCCCGGTTACT1390.12235700075702892No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTA1380.12147673456453231No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGCCAGCATCCCAG1320.11619513740955265No Hit
GTACTAGTTCACTATCGGTCGATCACGAGTATTTAGCCTTGGAGGATGGTCCCCCCATCTTCAGACAGGATTACA1290.11355433883206281No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1270.11179380644706959No Hit
TCCCTATACGGTTAAGCTTGCCACTGAACGTAAGTCGCTGACCCATTATACAAAAGGTACGCCGTCACCCCATAA1230.10827274167708315No Hit
GTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTAT1210.10651220929208992No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1170.10299114452210348Clontech Universal Primer Mix Long (96% over 26bp)
GTCCACGGTGGATGCCCTGGCACTGGAGCCGATGAAGGACGCGATTACCTGCGAAAAGCCCTGACGAGCCGGAGA1150.10123061213711025No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)