FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002150920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378636
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27330.7218014134947549No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG27230.7191603545357547Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG26300.6945985062170529Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT16650.4397363166735334Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15560.41094877402043123No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11750.31032442768252355No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10550.27863172017452115Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG9910.2617289428369199Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA9890.26120073104511987Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG9880.26093662514921984Clontech Universal Primer Mix Long (96% over 26bp)
CCCCTAGGCCCAACCCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCATTTTGCCGACTTCCCTTATCTACA8550.2258105409945172No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC8480.22396179972321703Clontech Universal Primer Mix Long (96% over 26bp)
GTGTCAACATCCTTTGGGACCATCACAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTT7520.19860763371681509No Hit
GTTCTGAGTTGGTTGTTCGTCGCCTAGGGAGCGGCGTGAGCCTTTCTCCGCCCGTCCGCCGGCGGCACGCGGCCG7320.19332551579881468No Hit
ACCTATACTCGGCCATGGGCGCAAGTGCGAAGCTCCCATGAGTAGGAGGGCGTGGGTGTCGTGGCGCAGCCTGCG7310.19306140990291465No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7090.18725108019311423No Hit
ATGTAGATAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGTTGGGCCTAGGG7090.18725108019311423No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG7050.18619465660951415Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCTACATTGTTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCTGGCAAGA6860.18117664458741378No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCTGGCAAGATCGGAAATGCTCCCCCGGA6690.17668684435711343No Hit
ATCCTGTATCAAGGGCGCGATTCGCGCCGCCCATCGAAAGGGAAAAGGGTTAAGATTCCCTTACCTGGACAAGGC6600.17430989129401325No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC6530.1724611500227131Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT6480.171140620543213Clontech Universal Primer Mix Long (96% over 26bp)
TCACAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTTGGTTGTTCGTCGCCT6360.16797134979241277No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG6110.16136870239491224Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCTCTAGTCGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGATTGGCT6010.15872764343591206No Hit
CCCCTTGTCCGTACCAGTTCTGAGTTGGTTGTTCGTCGCCTAGGGAGCGGCGTGAGCCTTTCTCCGCCCGTCCGC5750.15186089014251153No Hit
TACCTGGACAAGGCGGCTGACGGCAACGTAAGCGAGCCCGGAGACGCCGGCGGTGGCCCTGGGAAGAGTTCTCTT5700.15054036066301144No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC5590.1476351958081112Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACATCCTTTGGGACCATCACAATGCTTTGTTTTA5580.1473710899122112No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC5410.14288128968191086Clontech Universal Primer Mix Long (96% over 26bp)
CCTTTGGGACCATCACAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTTGGT5200.13733506586801045No Hit
TCCGGAGATAGGGCTCAGCAGCTGGTAAAGCACCGCACGTCTCGCGGTGTCCGGCGCGCCACCGACGGTCCTTGA4920.12994010078280988No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG4810.12703493592790965Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACATCCTTTGGGACCATCACAATGCTTTGTTTTAATTAAA4800.12677083003200962No Hit
GCCTAGGGGTCTGCGCCCGGAAACCGGCGGCTGTCGGACGGCTGCCCGAGCTGCTCACGCGGCGAGGGCGGACGG4670.12333745338530937No Hit
ATCCTAAGAGATGGGGCAATCCTGTATCAAGGGCGCGATTCGCGCCGCCCATCGAAAGGGAAAAGGGTTAAGATT4340.11462195882060872No Hit
GTGCTTTACCAGCTGCTGAGCCCTATCTCCGGATGATCCGATTCCAGGGCCTTCGAGCTGTTAAAAAGAAAAGAG4330.11435785292470868No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT4290.11330142934110861Clontech SMART CDS Primer II A (96% over 26bp)
GTCCGTACCAGTTCTGAGTTGGTTGTTCGTCGCCTAGGGAGCGGCGTGAGCCTTTCTCCGCCCGTCCGCCGGCGG4190.11066037038210841No Hit
GTCTTACGGTAGGGGAGCGTTCCGCTCTAGGGTGAAGTATTTATGAAAATAAGTATGGACGAAGCGGAAGTGAGA4080.10775520552720819No Hit
AAATCACATTGTGTCAACATCCTTTGGGACCATCACAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTC3880.1024730876092078No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)