FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002150921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378636
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG21070.5564711226613424Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG19540.5160629205886392Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT12090.31930402814312425Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11420.30160893311782294No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG8240.21762325822161652Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC7990.21102061082411602Clontech Universal Primer Mix Long (96% over 26bp)
CCCCTAGGCCCAACCCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCATTTTGCCGACTTCCCTTATCTACA7450.19675889244551498No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA7420.1959665747578149Clontech Universal Primer Mix Long (96% over 26bp)
ACCTATACTCGGCCATGGGCGCAAGTGCGAAGCTCCCATGAGTAGGAGGGCGTGGGTGTCGTGGCGCAGCCTGCG7200.19015624504801446No Hit
GTGTCAACATCCTTTGGGACCATCACAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTT6420.16955598516781287No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG6360.16797134979241277Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC6110.16136870239491224Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5900.15582247858101184No Hit
GCCTCTAGTCGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGATTGGCT5860.15476605499741175No Hit
ATGTAGATAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGTTGGGCCTAGGG5830.1539737373097117No Hit
ATCCTGTATCAAGGGCGCGATTCGCGCCGCCCATCGAAAGGGAAAAGGGTTAAGATTCCCTTACCTGGACAAGGC5600.14789930170401125No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG5500.14525824274501103Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCTGAGTTGGTTGTTCGTCGCCTAGGGAGCGGCGTGAGCCTTTCTCCGCCCGTCCGCCGGCGGCACGCGGCCG5440.14367360736961093No Hit
TCACAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTTGGTTGTTCGTCGCCT5320.14050433661881068No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG5280.1394479130352106Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT5200.13733506586801045Clontech Universal Primer Mix Long (96% over 26bp)
TATCTACATTGTTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCTGGCAAGA5150.13601453638851033No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC5060.13363758332541015Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACATCCTTTGGGACCATCACAATGCTTTGTTTTA5040.1331093715336101No Hit
CCCCTTGTCCGTACCAGTTCTGAGTTGGTTGTTCGTCGCCTAGGGAGCGGCGTGAGCCTTTCTCCGCCCGTCCGC4960.13099652436640996No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCTGGCAAGATCGGAAATGCTCCCCCGGA4920.12994010078280988No Hit
TACCTGGACAAGGCGGCTGACGGCAACGTAAGCGAGCCCGGAGACGCCGGCGGTGGCCCTGGGAAGAGTTCTCTT4650.12280924159350932No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4620.12201692390580926No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC4490.11858354725910901Clontech Universal Primer Mix Long (96% over 26bp)
GTCTTACGGTAGGGGAGCGTTCCGCTCTAGGGTGAAGTATTTATGAAAATAAGTATGGACGAAGCGGAAGTGAGA4470.11805533546730897No Hit
ATCCTAAGAGATGGGGCAATCCTGTATCAAGGGCGCGATTCGCGCCGCCCATCGAAAGGGAAAAGGGTTAAGATT4260.11250911165340854No Hit
TCCGGAGATAGGGCTCAGCAGCTGGTAAAGCACCGCACGTCTCGCGGTGTCCGGCGCGCCACCGACGGTCCTTGA4260.11250911165340854No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG4240.11198089986160852Clontech Universal Primer Mix Long (96% over 25bp)
GCCTAGGGGTCTGCGCCCGGAAACCGGCGGCTGTCGGACGGCTGCCCGAGCTGCTCACGCGGCGAGGGCGGACGG4150.10960394679850832No Hit
CCTTTGGGACCATCACAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTTGGT4110.10854752321490825No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC3940.1040577229846079Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACATCCTTTGGGACCATCACAATGCTTTGTTTTAATTAAA3850.10168076992150774No Hit
ATCACATTGTGTCAACATCCTTTGGGACCATCACAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCG3830.10115255812970769No Hit
GTCCGTACCAGTTCTGAGTTGGTTGTTCGTCGCCTAGGGAGCGGCGTGAGCCTTTCTCCGCCCGTCCGCCGGCGG3790.1000961345461076No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)