FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002150922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1236338
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG323172.613929200590777Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG284172.2984814832189904Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT177981.4395739676366819Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC126131.0201902715923963Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG116740.9442401673328814Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA112140.9070335134890297Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG100290.8111859378260637Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC96280.7787514417578365Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG88970.7196252157581503Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT76410.6180348739584159Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC72190.5839018132581867Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG70560.5707177163526479Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69120.5590704160189204No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC56180.4544064810755635Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG55280.4471269183669838Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC53960.43645022639440023Clontech Universal Primer Mix Long (96% over 26bp)
GTTCTAAGTTGGTTGTTAAACGTGCGCCGGACGGCCGAAGCCTGCCAAGGGTGTCAGCACCCCAGCGCTGAAAGG49820.40296423793493363No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT46560.3765960441238561Clontech SMART CDS Primer II A (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39730.32135225156874575No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC37110.30016063568376933Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33760.27306448560183383No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTT33710.2726600654513571No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC33520.27112326887954585Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG31280.253005246138192Clontech Universal Primer Mix Long (96% over 26bp)
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTTG31130.25179198568676203No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG31050.25114491344599943Clontech Universal Primer Mix Long (96% over 26bp)
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT30650.2479095522421862No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCATGA30300.24507861118884966No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG30140.24378446670732437Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCATGAAAACTATTTCTTCAAGCGG29490.23852700475112792No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC27830.22510025575530318Clontech Universal Primer Mix Long (96% over 26bp)
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGCGCCGGACGGCCGAAGCCTGCCAAGGGTGTCAGCA26620.2153132881137682No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGT26480.21418091169243364Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAG25500.20625427674309127No Hit
ATCCTAAGAGATAGGGTAATTCCGTTTTAATGTCGGCGCTTGCGCCGCGCCCTCGAAAGGGAAACCGGTTAAAAT24880.2012394668771808No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG24440.19768056955298632Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT23950.19371725207831514Clontech Universal Primer Mix Long (96% over 26bp)
GTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGCGCCGGACGGCCGAAGCCTGCCAAGGGTGTCAGCACCCCAG23540.1904010068444066No Hit
ACTTATACCCCTCCGCCGGGGCAAAATTTACGCCCCGGCGAGTAGGCAGGCGTGGAGGTCCGTGACGAAGCCTTG23450.18967305057354866No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA22020.1781066342699165No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA21980.17778309814953516Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGA21790.1762463015777239Clontech SMART CDS Primer II A (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG21630.17495215709619863Clontech Universal Primer Mix Long (96% over 26bp)
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGC21330.17252563619333872No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTT21320.1724447521632434Clontech SMART CDS Primer II A (100% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT20930.1692902749895255Clontech Universal Primer Mix Long (96% over 26bp)
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC20720.1675917103575236No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20370.16476076930418704No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTA20200.16338574079256646No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20080.16241513243142247Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA19970.16152540810037386Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC19510.15780474271598866No Hit
GTACATGGGACTGCCAACGCCGGCCTGGTAAGGTTCTGGGCCAAAGGCCTATTCGTATCCGACTGGTGCAGGTGG19470.15748120659560735No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA19410.15699590241503536Clontech Universal Primer Mix Long (96% over 26bp)
AAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT18580.150282527917123No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT18320.14817954313464443Clontech Universal Primer Mix Long (96% over 26bp)
GCCGAAGCCTGCCAAGGGTGTCAGCACCCCAGCGCTGAAAGGCGCCCACCTTGCGGTTAGTGCCCTTCTGCGCCA18090.14631921044245183No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGCGCCGGACGGCCG18020.14575302223178452No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTT17360.14041467624549273No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC17360.14041467624549273Clontech SMART CDS Primer II A (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC17350.1403337922153974Clontech Universal Primer Mix Long (96% over 26bp)
GTTGGACACGGGTCAATCGATCCTAAGAGATAGGGTAATTCCGTTTTAATGTCGGCGCTTGCGCCGCGCCCTCGA16760.13556163443977293No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA16610.13434837398834298Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCCCGGGTCGAACGGCCTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAACTTTGAGGACTGA16540.13378218577767567No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT16010.1294953321826232No Hit
GCCTTGGGGGTGACCCCGGGTCGAACGGCCTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAAC15820.12795853561081194No Hit
CTTCCAGCCATTGAACCCTAGCTCCGGACAAACCGATTTCAGGGTGACAGACTGTTAAGAAGAAAAGAGAACTCT15810.1278776515807166No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT15810.1278776515807166Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG15680.12682615918947732Clontech Universal Primer Mix Long (96% over 26bp)
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGCGCCGGACGGCCGAAGCCTGCCAAGGG15540.1256937827681427No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC15510.1254511306778567Clontech Universal Primer Mix Long (96% over 25bp)
GTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCATGAAAACTATTTC15430.12480405843709406No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT15340.12407610216623609Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACGACCTGGCATGAAAACTATTTCTTCAAGCGGATTTTCAAGGCTCGTCGGGAGCGCACCAAACGCCGCAA14830.11995101663137427No Hit
CCCCAAGGCCCAACGCACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCC14690.11881864021003966No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC14490.11720095960813305Clontech Universal Primer Mix Long (96% over 26bp)
CCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCATGAAAACTATTTCTTCAAGCGGATTTTCAAGGCTCGT14030.11348029422374786No Hit
GCGCTGAAAGGCGCCCACCTTGCGGTTAGTGCCCTTCTGCGCCAGAGGCGTCTCCCCAAGGCCCAACGCACCCAA13260.10725222390640747No Hit
TCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCG13260.10725222390640747No Hit
GGCCTATTCGTATCCGACTGGTGCAGGTGGAAATCACCCCGTTCTGGCATGGATTCTGACTTAGAGGCGTTCAGC13030.10539189121421487No Hit
CTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAACTTTGAGGACTGAAGTGGGGAAAGGTTCCG12670.102480066130783No Hit
GTCCTAAGGGGGACTCATGGAGAACAGAAATCTCCAGTAGAGCAAAAGGGCAAAAGTCCCCTTGATTTTGATTTT12420.10045796537839977No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)