FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002150938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384680
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGCCAGCATCCCAGCGCTGAAAGG25350.6589892897993137No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTG22460.5838619111989186No Hit
TATCTACATTGTTCTATCAACTAGAGGCTTTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTAA21240.5521472392638037No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT17460.4538837475304149No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGCCAGCA15580.4050119579910575No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGCCAGCATCCCAG14030.3647187272538213No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAG13260.34470209004887176No Hit
ATCCTAAGAGATAGGGAAACTCCGTTTTAATGTGGGCGCTTGCGCCCCACCCTCGAAAGGGAAGCCGGTTAACAT12790.3324841426640324No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAAAAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC12610.3278049287719663No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC12600.3275449724446293No Hit
GCCGAAGCCTGCCAAGGGTGCCAGCATCCCAGCGCTGAAAGGTGCCCACCTTGCGGCTAGTACCCTTCTGCGCCG11910.3096079858583758No Hit
GTTCTATCAACTAGAGGCTTTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTAAAAACTATTTC11850.30804824789435375No Hit
GAGTACGACCTGGCGTAAAAACTATTTCTTCAAGCGGATTTTCAAGGCTCGTCGGGAGCGCACCAAACGCCGCAA11580.3010294270562546No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT10370.2695747114484766No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA10260.2667151918477696No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTAC10190.26489549755641056No Hit
AAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT9840.2557970260996153No Hit
GTACCCTTCTGCGCCGAAAGCGTCTCCCCAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGT9330.2425392534054279No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCG9290.24149942809607985No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTA9040.2350005199126547No Hit
CCCCAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCC9020.23448060725798067No Hit
CTTCCAGCCATTGAACCCTAGCTCCGGACAAACCGATTTCAGGGTGACAGACTGTTAAGAAGAAAAGAGAACTCT8790.22850161172922948No Hit
CGTCGGGAGCGCACCAAACGCCGCAAAAGTGCAGCGCTCTTCCAGCCATTGAACCCTAGCTCCGGACAAACCGAT8580.22304252885515233No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGG8470.22018300925444526No Hit
GTCTGTCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTCAATGGCTGGAAGAGCGCTGCACTTTTGCGGCGTTT8080.21004471248830198No Hit
GAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTA8000.2079650618696059No Hit
GGTTAGTCGATCCTAAGAGATAGGGAAACTCCGTTTTAATGTGGGCGCTTGCGCCCCACCCTCGAAAGGGAAGCC7900.20536549859623585No Hit
GTGCCCACCTTGCGGCTAGTACCCTTCTGCGCCGAAAGCGTCTCCCCAAGGCCCAACGTACCCAACCCTTAGAGC7760.20172611001351773No Hit
GCCTTGGGGGTGACCCCGGGTCGAACGGCCTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAAC7470.19418737652074453No Hit
GTGACAGACTGTTAAGAAGAAAAGAGAACTCTTCTTGGGGCTCCCGCCAAGGTCTCCGCTCTCAGTTGCGTTGCC7330.1905479879380264No Hit
GCGCTGAAAGGTGCCCACCTTGCGGCTAGTACCCTTCTGCGCCGAAAGCGTCTCCCCAAGGCCCAACGTACCCAA7310.1900280752833524No Hit
GTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGCCAGCATCCCAGCGCTGAAAGGTGCCCACCTTG7290.18950816262867837No Hit
CCCTTATCTACATTGTTCTATCAACTAGAGGCTTTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGC7290.18950816262867837No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTT7230.18794842466465636No Hit
ACCCCGGGTCGAACGGCCTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAACTTTGAGGACTGA7170.1863886867006343No Hit
GCGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGCCAGCATCCCAGCGCTGAAAGGTGCCCACCTTGCGGCTAGT7140.18560881771862328No Hit
CCCTAGCTCCGGACAAACCGATTTCAGGGTGACAGACTGTTAAGAAGAAAAGAGAACTCTTCTTGGGGCTCCCGC7020.18248934179057918No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACCCA7000.18196942913590516No Hit
ACATTGTTCTATCAACTAGAGGCTTTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTAAAAACT6990.18170947280856817No Hit
GTTTTAATGTGGGCGCTTGCGCCCCACCCTCGAAAGGGAAGCCGGTTAACATTCCGGCACCTGGATGTAGATTCT6920.1798897785172091No Hit
GGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCT6840.17781012789851305No Hit
GCTCTAAGGGTTGGGTACGTTGGGCCTTGGGGAGACGCTTTCGGCGCAGAAGGGTACTAGCCGCAAGGTGGGCAC6820.17729021524383903No Hit
GATGTAGATTCTCCGCGGCAACGCAACTGAGAGCGGAGACCTTGGCGGGAGCCCCAAGAAGAGTTCTCTTTTCTT6780.17625038993449102No Hit
GAAGGGTACTAGCCGCAAGGTGGGCACCTTTCAGCGCTGGGATGCTGGCACCCTTGGCAGGCTTCGGCCGTCCGG6700.17417073931579494No Hit
ATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGTAACGTTTTCAGTTTTATGAGGTAAAGCGAAT6640.1726110013517729No Hit
CCTTGCGGCTAGTACCCTTCTGCGCCGAAAGCGTCTCCCCAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCT6460.16793178745970677No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTGGGCC6410.16663200582302173No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAGTATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATT6410.16663200582302173No Hit
CTTCTTAACAGTCTGTCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTCAATGGCTGGAAGAGCGCTGCACTTT6410.16663200582302173No Hit
CTCTAAGGGTTGGGTACGTTGGGCCTTGGGGAGACGCTTTCGGCGCAGAAGGGTACTAGCCGCAAGGTGGGCACC6380.16585213684101072No Hit
CTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTT6380.16585213684101072No Hit
GCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGT6110.1588333160029115No Hit
GTTATGAGTACGACCTGGCGTAAAAACTATTTCTTCAAGCGGATTTTCAAGGCTCGTCGGGAGCGCACCAAACGC6050.15727357803888947No Hit
GTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACCCATACCCCGCCG6030.15675366538421545No Hit
CTTCTTGGGGCTCCCGCCAAGGTCTCCGCTCTCAGTTGCGTTGCCGCGGAGAATCTACATCCAGGTGCCGGAATG6030.15675366538421545No Hit
ATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCC5970.15519392742019342No Hit
ACTCATAACCGCAGCAGGTCTCCAAGGTGAAAAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAA5950.1546740147655194No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5810.15103462618280128No Hit
GCTCCGGACAAACCGATTTCAGGGTGACAGACTGTTAAGAAGAAAAGAGAACTCTTCTTGGGGCTCCCGCCAAGG5790.1505147135281273No Hit
ACTAGAGGCTTTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTAAAAACTATTTCTTCAAGCGG5790.1505147135281273No Hit
CATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATGA5780.1502547572007903No Hit
CTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAACTTTGAGGACTGAAGTGGGGAAAGGTTCCG5720.14869501923676823No Hit
GCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGAC5710.14843506290943123No Hit
GGCTAGTACCCTTCTGCGCCGAAAGCGTCTCCCCAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCC5660.14713528127274617No Hit
GCCTTGGGGAGACGCTTTCGGCGCAGAAGGGTACTAGCCGCAAGGTGGGCACCTTTCAGCGCTGGGATGCTGGCA5660.14713528127274617No Hit
TCCCAGCGCTGAAAGGTGCCCACCTTGCGGCTAGTACCCTTCTGCGCCGAAAGCGTCTCCCCAAGGCCCAACGTA5620.14609545596339815No Hit
GTTAAGAAGAAAAGAGAACTCTTCTTGGGGCTCCCGCCAAGGTCTCCGCTCTCAGTTGCGTTGCCGCGGAGAATC5600.14557554330872413No Hit
ACCCTCGAAAGGGAAGCCGGTTAACATTCCGGCACCTGGATGTAGATTCTCCGCGGCAACGCAACTGAGAGCGGA5480.14245606738068006No Hit
TGCCAAGGGTGCCAGCATCCCAGCGCTGAAAGGTGCCCACCTTGCGGCTAGTACCCTTCTGCGCCGAAAGCGTCT5470.14219611105334304No Hit
TAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGC5450.14167619839866902No Hit
CTCTTAGGATCGACTAACCCGTGTCCAACTGCTGTTCACACGGAACCTTTCCCCACTTCAGTCCTCAAAGTTCTC5420.140896329416658No Hit
CCCCCAAGGCTTCGTCACGGACCTCCACGCCTGCCTACTCGCTGGGGCATAAATCCTGCCCCAGCGGCGGGGTAT5340.13881667879796195No Hit
GGATGTAGATTCTCCGCGGCAACGCAACTGAGAGCGGAGACCTTGGCGGGAGCCCCAAGAAGAGTTCTCTTTTCT5270.1369969845066029No Hit
AACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATT5200.13517729021524386No Hit
CTCATAACCGCAGCAGGTCTCCAAGGTGAAAAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAA5140.1336175522512218No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT5110.13283768326921078No Hit
ACCCGGGGTCACCCCCAAGGCTTCGTCACGGACCTCCACGCCTGCCTACTCGCTGGGGCATAAATCCTGCCCCAG5090.13231777061453676No Hit
ACTGAGAGCGGAGACCTTGGCGGGAGCCCCAAGAAGAGTTCTCTTTTCTTCTTAACAGTCTGTCACCCTGAAATC5070.13179785795986274No Hit
GGGTGCCAGCATCCCAGCGCTGAAAGGTGCCCACCTTGCGGCTAGTACCCTTCTGCGCCGAAAGCGTCTCCCCAA5060.13153790163252574No Hit
GTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACCCATACCCCGC5050.13127794530518871No Hit
CTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTAGATGAACTAACACCTTTTGTGGTGTCTGAT5000.1299781636685037No Hit
ATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGG4970.12919829468649266No Hit
ACCCATACCCCGCCGCTGGGGCAGGATTTATGCCCCAGCGAGTAGGCAGGCGTGGAGGTCCGTGACGAAGCCTTG4880.1268586877404596No Hit
CCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTAAAAACTATTTCTTCAAGCGGATTTTCAAGGCTCGT4870.12659873141312258No Hit
ACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCT4870.12659873141312258No Hit
GAGCTAGGGTTCAATGGCTGGAAGAGCGCTGCACTTTTGCGGCGTTTGGTGCGCTCCCGACGAGCCTTGAAAATC4870.12659873141312258No Hit
CCCATACCCCGCCGCTGGGGCAGGATTTATGCCCCAGCGAGTAGGCAGGCGTGGAGGTCCGTGACGAAGCCTTGG4840.12581886243111157No Hit
CTTCTGCGCCGAAAGCGTCTCCCCAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGG4830.12555890610377457No Hit
ACGCAACTGAGAGCGGAGACCTTGGCGGGAGCCCCAAGAAGAGTTCTCTTTTCTTCTTAACAGTCTGTCACCCTG4670.12139960486638245No Hit
GGCTGGAAGAGCGCTGCACTTTTGCGGCGTTTGGTGCGCTCCCGACGAGCCTTGAAAATCCGCTTGAAGAAATAG4660.12113964853904544No Hit
GGGTACGTTGGGCCTTGGGGAGACGCTTTCGGCGCAGAAGGGTACTAGCCGCAAGGTGGGCACCTTTCAGCGCTG4570.11880004159301237No Hit
GTCCGGAGCTAGGGTTCAATGGCTGGAAGAGCGCTGCACTTTTGCGGCGTTTGGTGCGCTCCCGACGAGCCTTGA4550.11828012893833836No Hit
GTACTAGCCGCAAGGTGGGCACCTTTCAGCGCTGGGATGCTGGCACCCTTGGCAGGCTTCGGCCGTCCGGCGTAC4540.11802017261100135No Hit
TCTTAACAGTCTGTCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTCAATGGCTGGAAGAGCGCTGCACTTTTG4500.11698034730165331No Hit
CAGTTGCGTTGCCGCGGAGAATCTACATCCAGGTGCCGGAATGTTAACCGGCTTCCCTTTCGAGGGTGGGGCGCA4500.11698034730165331No Hit
TTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTG4470.1162004783196423No Hit
CCCAAGAAGAGTTCTCTTTTCTTCTTAACAGTCTGTCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTCAATGG4440.11542060933763128No Hit
GGGTTAAGGTGCCAGAATATACGCTCATCAGACACCACAAAAGGTGTTAGTTCATCTAGACAGCAGGACGGTGGC4390.11412082770094624No Hit
CGTTGGGCCTTGGGGAGACGCTTTCGGCGCAGAAGGGTACTAGCCGCAAGGTGGGCACCTTTCAGCGCTGGGATG4380.11386087137360923No Hit
GGGTTGGGTACGTTGGGCCTTGGGGAGACGCTTTCGGCGCAGAAGGGTACTAGCCGCAAGGTGGGCACCTTTCAG4360.11334095871893522No Hit
ATCTACATCCAGGTGCCGGAATGTTAACCGGCTTCCCTTTCGAGGGTGGGGCGCAAGCGCCCACATTAAAACGGA4360.11334095871893522No Hit
CTGTTAAGAAGAAAAGAGAACTCTTCTTGGGGCTCCCGCCAAGGTCTCCGCTCTCAGTTGCGTTGCCGCGGAGAA4330.1125610897369242No Hit
CGGGTTAGTCGATCCTAAGAGATAGGGAAACTCCGTTTTAATGTGGGCGCTTGCGCCCCACCCTCGAAAGGGAAG4300.11178122075491317No Hit
GTTGAATGTATCGTTACTAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGAACCGAACGCGGGGTT4280.11126130810023915No Hit
TGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGCCAGCATCCCA4250.11048143911822814No Hit
GTTGGACACGGGTTAGTCGATCCTAAGAGATAGGGAAACTCCGTTTTAATGTGGGCGCTTGCGCCCCACCCTCGA4220.10970157013621712No Hit
TCTCCAAGGTGAAAAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCG4220.10970157013621712No Hit
TCCCTTATCTACATTGTTCTATCAACTAGAGGCTTTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGG4220.10970157013621712No Hit
AGGTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAAAAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAG4170.10840178849953208No Hit
GTACGACCTGGCGTAAAAACTATTTCTTCAAGCGGATTTTCAAGGCTCGTCGGGAGCGCACCAAACGCCGCAAAA4140.10762191951752106No Hit
CCCGACGAGCCTTGAAAATCCGCTTGAAGAAATAGTTTTTACGCCAGGTCGTACTCATAACCGCAGCAGGTCTCC4130.10736196319018405No Hit
CTTGCGCCCCACCCTCGAAAGGGAAGCCGGTTAACATTCCGGCACCTGGATGTAGATTCTCCGCGGCAACGCAAC4070.105802225226162No Hit
GATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTGGGCCT4070.105802225226162No Hit
CATCAGACACCACAAAAGGTGTTAGTTCATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGA4070.105802225226162No Hit
GCACTAGAGGCCGTTCGACCCGGGGTCACCCCCAAGGCTTCGTCACGGACCTCCACGCCTGCCTACTCGCTGGGG3990.10372257460746594No Hit
GCTGAAAGGTGCCCACCTTGCGGCTAGTACCCTTCTGCGCCGAAAGCGTCTCCCCAAGGCCCAACGTACCCAACC3990.10372257460746594No Hit
GTCTAGATGAACTAACACCTTTTGTGGTGTCTGATGAGCGTATATTCTGGCACCTTAACCCCGCGTTCGGTTCAT3940.10242279297078091No Hit
CCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAACT3920.10190288031610689No Hit
GGATAGCAGTAACGTTTTCAGTTTTATGAGGTAAAGCGAATGATTAGAGGCCTGGGGGTTGAAACAACCTTCACC3870.10060309867942185No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)