FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002150939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384680
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTG20330.528491213476136No Hit
GTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGCCAGCATCCCAGCGCTGAAAGG20080.5219923052927109No Hit
TATCTACATTGTTCTATCAACTAGAGGCTTTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTAA14960.38889466569616304No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT14360.3732972860559426No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGCCAGCA12750.33144431735468444No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGCCAGCATCCCAG11460.29790995112821045No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAAAAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC10870.28257252781532705No Hit
ATCCTAAGAGATAGGGAAACTCCGTTTTAATGTGGGCGCTTGCGCCCCACCCTCGAAAGGGAAGCCGGTTAACAT10620.27607361963190186No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAG10330.2685348861391286No Hit
CTCCCGACGAGCCTTGAAAATCCGCTTGAAGAAATAGTTTTTACGCCAGGTCGTACTCATAACCGCAGCAGGTCT9790.2544972444629302No Hit
GGCTGGAAGAGCGCTGCACTTTTGCGGCGTTTGGTGCGCTCCCGACGAGCCTTGAAAATCCGCTTGAAGAAATAG9600.24955807424352708No Hit
AAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT9340.2427992097327649No Hit
GTCTGTCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTCAATGGCTGGAAGAGCGCTGCACTTTTGCGGCGTTT9190.23889986482270978No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA9100.23656025787667673No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTAC8920.2318810439846106No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT8760.22772174274721846No Hit
GAGTACGACCTGGCGTAAAAACTATTTCTTCAAGCGGATTTTCAAGGCTCGTCGGGAGCGCACCAAACGCCGCAA8660.2251221794738484No Hit
GCCGAAGCCTGCCAAGGGTGCCAGCATCCCAGCGCTGAAAGGTGCCCACCTTGCGGCTAGTACCCTTCTGCGCCG8390.21810335863574917No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTA8140.211604450452324No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC7860.20432567328688783No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCG7830.20354580430487676No Hit
GAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTA7760.20172611001351773No Hit
GTTCTATCAACTAGAGGCTTTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTAAAAACTATTTC7450.1936674638660705No Hit
GGTTAGTCGATCCTAAGAGATAGGGAAACTCCGTTTTAATGTGGGCGCTTGCGCCCCACCCTCGAAAGGGAAGCC7230.18794842466465636No Hit
GTCCGGAGCTAGGGTTCAATGGCTGGAAGAGCGCTGCACTTTTGCGGCGTTTGGTGCGCTCCCGACGAGCCTTGA7210.1874285120099823No Hit
CTTCTTAACAGTCTGTCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTCAATGGCTGGAAGAGCGCTGCACTTT7100.18456899240927524No Hit
GAGCTAGGGTTCAATGGCTGGAAGAGCGCTGCACTTTTGCGGCGTTTGGTGCGCTCCCGACGAGCCTTGAAAATC7080.18404907975460122No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGG6990.18170947280856817No Hit
CTTCCAGCCATTGAACCCTAGCTCCGGACAAACCGATTTCAGGGTGACAGACTGTTAAGAAGAAAAGAGAACTCT6880.17884995320786107No Hit
GTACCCTTCTGCGCCGAAAGCGTCTCCCCAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGT6770.175990433607154No Hit
CCCCAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCC6600.17157117604242486No Hit
GTGACAGACTGTTAAGAAGAAAAGAGAACTCTTCTTGGGGCTCCCGCCAAGGTCTCCGCTCTCAGTTGCGTTGCC6260.16273266091296662No Hit
CGTCGGGAGCGCACCAAACGCCGCAAAAGTGCAGCGCTCTTCCAGCCATTGAACCCTAGCTCCGGACAAACCGAT6160.16013309763959654No Hit
ATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGTAACGTTTTCAGTTTTATGAGGTAAAGCGAAT6150.15987314131225952No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACCCA6040.15701362171155245No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTT5930.15415410211084538No Hit
GTTTTAATGTGGGCGCTTGCGCCCCACCCTCGAAAGGGAAGCCGGTTAACATTCCGGCACCTGGATGTAGATTCT5890.15311427680149736No Hit
GCTCTAAGGGTTGGGTACGTTGGGCCTTGGGGAGACGCTTTCGGCGCAGAAGGGTACTAGCCGCAAGGTGGGCAC5880.15285432047416034No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAGTATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATT5870.15259436414682334No Hit
CTCTAAGGGTTGGGTACGTTGGGCCTTGGGGAGACGCTTTCGGCGCAGAAGGGTACTAGCCGCAAGGTGGGCACC5860.15233440781948632No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTGGGCC5770.14999480087345324No Hit
GTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGCCAGCATCCCAGCGCTGAAAGGTGCCCACCTTG5650.14687532494540917No Hit
TCTTAACAGTCTGTCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTCAATGGCTGGAAGAGCGCTGCACTTTTG5640.14661536861807217No Hit
GAAGGGTACTAGCCGCAAGGTGGGCACCTTTCAGCGCTGGGATGCTGGCACCCTTGGCAGGCTTCGGCCGTCCGG5640.14661536861807217No Hit
GATGTAGATTCTCCGCGGCAACGCAACTGAGAGCGGAGACCTTGGCGGGAGCCCCAAGAAGAGTTCTCTTTTCTT5550.1442757616720391No Hit
GTGCCCACCTTGCGGCTAGTACCCTTCTGCGCCGAAAGCGTCTCCCCAAGGCCCAACGTACCCAACCCTTAGAGC5500.14297598003535406No Hit
GCGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGCCAGCATCCCAGCGCTGAAAGGTGCCCACCTTGCGGCTAGT5370.13959654777997296No Hit
ACTCATAACCGCAGCAGGTCTCCAAGGTGAAAAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAA5290.1375168971612769No Hit
GCGCTGAAAGGTGCCCACCTTGCGGCTAGTACCCTTCTGCGCCGAAAGCGTCTCCCCAAGGCCCAACGTACCCAA5200.13517729021524386No Hit
CCCTAGCTCCGGACAAACCGATTTCAGGGTGACAGACTGTTAAGAAGAAAAGAGAACTCTTCTTGGGGCTCCCGC5170.1343974212332328No Hit
CTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTT5120.13309763959654777No Hit
GCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGT5080.13205781428719973No Hit
GTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACCCATACCCCGCCG5040.13101798897785172No Hit
GGATGTAGATTCTCCGCGGCAACGCAACTGAGAGCGGAGACCTTGGCGGGAGCCCCAAGAAGAGTTCTCTTTTCT4930.12815846937714465No Hit
ACCCTCGAAAGGGAAGCCGGTTAACATTCCGGCACCTGGATGTAGATTCTCCGCGGCAACGCAACTGAGAGCGGA4880.1268586877404596No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT4860.1263387750857856No Hit
CCCAAGAAGAGTTCTCTTTTCTTCTTAACAGTCTGTCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTCAATGG4810.12503899344910055No Hit
CATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATGA4780.12425912446708952No Hit
GGGTACGTTGGGCCTTGGGGAGACGCTTTCGGCGCAGAAGGGTACTAGCCGCAAGGTGGGCACCTTTCAGCGCTG4720.1226993865030675No Hit
ACTGAGAGCGGAGACCTTGGCGGGAGCCCCAAGAAGAGTTCTCTTTTCTTCTTAACAGTCTGTCACCCTGAAATC4720.1226993865030675No Hit
CCCTTATCTACATTGTTCTATCAACTAGAGGCTTTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGC4560.11854008526567536No Hit
ACGCAACTGAGAGCGGAGACCTTGGCGGGAGCCCCAAGAAGAGTTCTCTTTTCTTCTTAACAGTCTGTCACCCTG4540.11802017261100135No Hit
CCTTGCGGCTAGTACCCTTCTGCGCCGAAAGCGTCTCCCCAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCT4540.11802017261100135No Hit
TAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGC4530.11776021628366436No Hit
GCCTTGGGGAGACGCTTTCGGCGCAGAAGGGTACTAGCCGCAAGGTGGGCACCTTTCAGCGCTGGGATGCTGGCA4470.1162004783196423No Hit
ATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCC4460.1159405219923053No Hit
GCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGAC4450.11568056566496829No Hit
GTACTAGCCGCAAGGTGGGCACCTTTCAGCGCTGGGATGCTGGCACCCTTGGCAGGCTTCGGCCGTCCGGCGTAC4410.11464074035562025No Hit
GCTCCGGACAAACCGATTTCAGGGTGACAGACTGTTAAGAAGAAAAGAGAACTCTTCTTGGGGCTCCCGCCAAGG4390.11412082770094624No Hit
CTCATAACCGCAGCAGGTCTCCAAGGTGAAAAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAA4390.11412082770094624No Hit
GGGTTGGGTACGTTGGGCCTTGGGGAGACGCTTTCGGCGCAGAAGGGTACTAGCCGCAAGGTGGGCACCTTTCAG4360.11334095871893522No Hit
TCCCAGCGCTGAAAGGTGCCCACCTTGCGGCTAGTACCCTTCTGCGCCGAAAGCGTCTCCCCAAGGCCCAACGTA4330.1125610897369242No Hit
ATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGG4300.11178122075491317No Hit
CGTTGGGCCTTGGGGAGACGCTTTCGGCGCAGAAGGGTACTAGCCGCAAGGTGGGCACCTTTCAGCGCTGGGATG4300.11178122075491317No Hit
GTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACCCATACCCCGC4300.11178122075491317No Hit
ACATTGTTCTATCAACTAGAGGCTTTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTAAAAACT4240.11022148279089114No Hit
GTTAAGAAGAAAAGAGAACTCTTCTTGGGGCTCCCGCCAAGGTCTCCGCTCTCAGTTGCGTTGCCGCGGAGAATC4220.10970157013621712No Hit
ACCCGGGGTCACCCCCAAGGCTTCGTCACGGACCTCCACGCCTGCCTACTCGCTGGGGCATAAATCCTGCCCCAG4220.10970157013621712No Hit
CTTCTTGGGGCTCCCGCCAAGGTCTCCGCTCTCAGTTGCGTTGCCGCGGAGAATCTACATCCAGGTGCCGGAATG4130.10736196319018405No Hit
GGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCT4130.10736196319018405No Hit
GTTATGAGTACGACCTGGCGTAAAAACTATTTCTTCAAGCGGATTTTCAAGGCTCGTCGGGAGCGCACCAAACGC4080.10606218155349902No Hit
GCCTTGGGGGTGACCCCGGGTCGAACGGCCTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAAC4070.105802225226162No Hit
CATCAGACACCACAAAAGGTGTTAGTTCATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGA4050.105282312571488No Hit
TCTCCAAGGTGAAAAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCG4020.10450244358947695No Hit
GGGTGCCAGCATCCCAGCGCTGAAAGGTGCCCACCTTGCGGCTAGTACCCTTCTGCGCCGAAAGCGTCTCCCCAA4010.10424248726213996No Hit
AGGTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAAAAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAG4010.10424248726213996No Hit
CTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTAGATGAACTAACACCTTTTGTGGTGTCTGAT4000.10398253093480295No Hit
GGCTAGTACCCTTCTGCGCCGAAAGCGTCTCCCCAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCC3970.10320266195279193No Hit
CCCCCAAGGCTTCGTCACGGACCTCCACGCCTGCCTACTCGCTGGGGCATAAATCCTGCCCCAGCGGCGGGGTAT3970.10320266195279193No Hit
GGGTTAAGGTGCCAGAATATACGCTCATCAGACACCACAAAAGGTGTTAGTTCATCTAGACAGCAGGACGGTGGC3900.10138296766143287No Hit
CGGGTTAGTCGATCCTAAGAGATAGGGAAACTCCGTTTTAATGTGGGCGCTTGCGCCCCACCCTCGAAAGGGAAG3900.10138296766143287No Hit
CCTTGGCGGGAGCCCCAAGAAGAGTTCTCTTTTCTTCTTAACAGTCTGTCACCCTGAAATCGGTTTGTCCGGAGC3880.10086305500675886No Hit
ACCCATACCCCGCCGCTGGGGCAGGATTTATGCCCCAGCGAGTAGGCAGGCGTGGAGGTCCGTGACGAAGCCTTG3880.10086305500675886No Hit
GTTGGACACGGGTTAGTCGATCCTAAGAGATAGGGAAACTCCGTTTTAATGTGGGCGCTTGCGCCCCACCCTCGA3860.10034314235208486No Hit
ACTAGAGGCTTTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTAAAAACTATTTCTTCAAGCGG3850.10008318602474785No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)