FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002150943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252761
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12310.48702133636122663No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6440.25478614184941506No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4950.1958371742476094No Hit
CCCTATTAGTGTGTAGTGAACAACGAAGCATGAATGGGTTGCAAAAAATCGGTGGCTGCGACTGTTTATTAAAAA4910.1942546516274267No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG4070.16102167660358996Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT3620.14321829712653456No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG3590.14203140516139753Clontech Universal Primer Mix Long (96% over 26bp)
CTATTAGTGTGTAGTGAACAACGAAGCATGAATGGGTTGCAAAAAATCGGTGGCTGCGACTGTTTATTAAAAACA3470.13728383730084942No Hit
ATTCTAAGGCGCTTGAGAGAACTCAGGAGAAGGAACTCGGCAAATTGACACCGTAACTTCGGAAGAAGGTGTGCC3370.13332753075039266No Hit
GTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTAT3360.132931900095347No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3250.12857996288984455No Hit
TTCCAGCACCGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTAATAAACAG3200.12660180961461617No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGGACTTCAATCAAGAGCTTGCACCCCATCATTTAATCTT3000.11868919651370267No Hit
CGTATAGGGTGTGACGCCTGCCCGGTGCTGGAAGATTAAATGATGGGGTGCAAGCTCTTGATTGAAGTCCCAGTA2970.11750230454856564No Hit
ACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGGTGTGACGCCTGCCCGGTGCTGGAAGATTAAATGATGG2970.11750230454856564No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTATTGTTACGGC2850.11275473668801754No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTAATAAACAGTCGCAGCCACCGATTTTTTGCAAC2800.11077658341278915No Hit
AGTGTGTAGTGAACAACGAAGCATGAATGGGTTGCAAAAAATCGGTGGCTGCGACTGTTTATTAAAAACACAGCA2770.10958969144765213No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGCGTAACGACTTCCCCACTGTCTC2720.10761153817242376No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTA2660.1052377542421497No Hit
GTGCTGGAAGATTAAATGATGGGGTGCAAGCTCTTGATTGAAGTCCCAGTAAACGGCGGCCGTAACAATAACGGT2650.10484212358710403No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)