FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002150967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002150967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences298669
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG34001.138383963518142Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG30661.0265544800431248Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT18040.6040131382902142Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG12270.41082268330492955Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC12040.4031218506105421Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA10550.3532338475034235Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG9360.31339040878028857Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT8030.2688595066779612Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC7740.25914976110677707Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG7710.2581453046683787Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC7440.24910519672279346Clontech Universal Primer Mix Long (96% over 26bp)
CTCCATGGACCTTGAAAATCTATGAAAGGGATACGCAAATTCTCAATAGCCTGTACCGAATCCGCAGCAGGTCTC7110.2380561759004115No Hit
CCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGATGGCCTTTCATAGACGACACGTGAATGCCATCCATTA7050.23604726302361478No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG6800.22767679270362845Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTAATATACATCGCACCGCGAAGCACAATGCATATTGTAAAGCCCGAGCCACCACAGTCTTCAGAGCCAATCCT5710.191181542108488No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC5360.1794628836605071Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC4970.16640494996132843Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG4740.158704117266941Clontech Universal Primer Mix Long (96% over 25bp)
TTCATAGACGACACGTGAATGCCATCCATTACAGACTTAATATACATCGCACCGCGAAGCACAATGCATATTGTA4730.15836929845414155No Hit
TCCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGATGGCCTTTCATAGACGACACGTGAATGCCATCCATT4550.15234255982375136No Hit
GTATAACAGATTGGTAGAGCATGACATTAAATTGTTGTGTCGGTGCCCTCTCCATGGACCTTGAAAATCTATGAA4500.15066846575975412No Hit
ATGTAGGTAAGGGAAGTCGGCAAATTAGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGACTGTGGTGGCTC4470.14966400932135576No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT4190.14028908256297104Clontech SMART CDS Primer II A (96% over 26bp)
CTCATAGAGAAGCCGTTGGGAGGCATCGGAAGAGTTTTCTTTTCTGTTTTACAATCGTACGCACCATGGAAGTCT4110.1376105320605754No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC4050.1356016191837787Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC4020.13459716274538033Clontech Universal Primer Mix Long (96% over 26bp)
AGCCTGTACCGAATCCGCAGCAGGTCTCCAAGATTAAGAGTCTCTAGTCGATAGAACAATGTAGGTAAGGGAAGT3690.12354814192299837No Hit
AGCCACCACAGTCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATT3650.12220886667180056No Hit
GTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAAACCGTGCCAGTT3430.11484285279021259No Hit
TACCTACATTGTTCTATCGACTAGAGACTCTTAATCTTGGAGACCTGCTGCGGATTCGGTACAGGCTATTGAGAA3240.10848129534702296No Hit
CTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTGTTCTATCGACT3200.10714202009582513No Hit
CTCCAGAGCCGTCCACCCAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTAT3110.10412865078063006No Hit
CTTGTGCGCCGTCCCTCGAAAGGGAAGCCGGTTAATATTCCGGCACCTGGATTTGGATTCTCCACGGCAACGTAA3090.10345901315503116No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)