FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002151032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424168
Sequences flagged as poor quality0
Sequence length75
%GC31

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94362.224590256690745No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58661.3829426076460272No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38360.9043586503460892No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA33620.7926104750947738RNA PCR Primer, Index 37 (96% over 28bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23840.5620414552724392No Hit
TTATACACATCTCCGAGCCCACGAGACCGGAGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA10080.23764168914203807RNA PCR Primer, Index 28 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCGGAGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA9890.23316233190622584RNA PCR Primer, Index 37 (96% over 28bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9780.23056901982233452No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6920.16314290564116105No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT5360.12636502517870277No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTG5330.12565775824673242No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5220.12306444616284114No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT5150.12141415665491032No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4780.11269119782727599No Hit
GTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGTCTGCACCCCAGCGCTGAAAGG4710.11104090831934516No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4280.10090341562777012No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)