FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002151036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences292092
Sequences flagged as poor quality0
Sequence length75
%GC25

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81052.7748106760883555No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA47671.6320200484778769RNA PCR Primer, Index 37 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45771.5669720499020858No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37111.2704901195513743No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20970.7179244895443901No Hit
TTATACACATCTCCGAGCCCACGAGACCGGAGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA16320.5587280719773222RNA PCR Primer, Index 28 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCGGAGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA13500.46218314777535846RNA PCR Primer, Index 37 (96% over 28bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7560.25882256275420074No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT5630.19274749051668652No Hit
TATACACATCTCCGAGCCCACGAGACCGGAGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA5500.18829683798255342RNA PCR Primer, Index 37 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT5170.17699902770360024No Hit
ACACATCTCCGAGCCCACGAGACCGGAGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA5000.17117894362050312RNA PCR Primer, Index 37 (96% over 28bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4530.15508812292017582No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4460.1526916177094888Clontech SMART CDS Primer II A (100% over 21bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4420.15132218616052476No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTT3830.1311230708133054No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3810.13043835503882337No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3800.13009599715158238No Hit
GCGTTGATACCACTGCTTGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3790.12975363926434136No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTTTTTTTTTTTTTTTTTTTTTTTTT3720.12735713405365431No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGA3580.12256412363228024RNA PCR Primer, Index 37 (96% over 28bp)
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3400.11640168166194213No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTTTTTTTTTTTTTTTTTTTTTTTTTT3230.11058159757884503No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)