FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002151048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171301
Sequences flagged as poor quality0
Sequence length75
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49092.865715903584918No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29811.7402116741875413No Hit
CTTATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA22691.3245690334557298Illumina PCR Primer Index 11 (96% over 28bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18721.092813235182515No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12250.7151154984500966No Hit
ATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA7060.4121400342087904Illumina PCR Primer Index 11 (96% over 28bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6050.3531794910712722No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5280.30822937402583755No Hit
TTATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA4860.28371112836469137RNA PCR Primer, Index 45 (96% over 30bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4690.27378707654946555No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4000.2335071015347254No Hit
ACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA2760.16111990005896054Illumina PCR Primer Index 11 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT2290.1336828156286303No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT2270.13251528012095667No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2050.11967238953654677No Hit
TATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA2040.11908862178270996Illumina PCR Primer Index 11 (96% over 28bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1910.1114996409828314No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1760.10274312467527918No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)