FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002151050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138415
Sequences flagged as poor quality0
Sequence length75
%GC30

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33692.4339847559874292No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21151.5280135823429541No Hit
CTTATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA12800.9247552649640574Illumina PCR Primer Index 11 (96% over 28bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12360.8929668027309179No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10170.7347469566159737No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6630.4789943286493516No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4600.3323339233464581No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4460.32221941263591375No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3740.2702019289816855No Hit
ATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA3440.2485279774590904Illumina PCR Primer Index 11 (96% over 28bp)
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG2400.17339161218076074No Hit
TTATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA1910.13799082469385543RNA PCR Primer, Index 45 (96% over 30bp)
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTAT1670.12065166347577935No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1450.10475743235920962No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)