FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002151054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228409
Sequences flagged as poor quality0
Sequence length75
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA117935.1631065325797145Illumina PCR Primer Index 11 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA43741.9149858368102832RNA PCR Primer, Index 45 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA42261.8501897911203147Illumina PCR Primer Index 11 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA17180.752159503347066Illumina PCR Primer Index 11 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA14570.6378908011505676Illumina PCR Primer Index 11 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14440.632199256596719No Hit
CTTATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACA8460.37038820711968445Illumina PCR Primer Index 11 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8210.3594429291315141No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8000.350248895621451No Hit
CTTATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGA6810.2981493723977601Illumina PCR Primer Index 11 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAC5770.2526170159669715Illumina PCR Primer Index 11 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAA4420.19351251483085166Illumina PCR Primer Index 11 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAACA4030.17643788116930595Illumina PCR Primer Index 11 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAG3930.1720597699740378Illumina PCR Primer Index 11 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAGA3810.16680603653971604Illumina PCR Primer Index 11 (96% over 28bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3580.15673638079059932No Hit
ATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAACAA3280.1436020472047949Illumina PCR Primer Index 11 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACA2970.13002990249946367RNA PCR Primer, Index 45 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACATTCCTTCAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA2960.12959209137993688RNA PCR Primer, Index 45 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAG2740.11996024675034697Illumina PCR Primer Index 11 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAC2720.11908462451129334RNA PCR Primer, Index 45 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAC2710.11864681339176651Illumina PCR Primer Index 11 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAGA2670.11689556891365928RNA PCR Primer, Index 45 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAGAA2630.11514432443555202Illumina PCR Primer Index 11 (96% over 28bp)
GTATCAACGCAGAGTACATGGGAAGCACTGGTATCAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATT2370.10376123532785486No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)